Search results for "2-3"
showing 10 items of 119 documents
Replication of subgenomic hepatitis C virus RNAs in a hepatoma cell line.
1999
An estimated 170 million persons worldwide are infected with hepatitis C virus (HCV), a major cause of chronic liver disease. Despite increasing knowledge of genome structure and individual viral proteins, studies on virus replication and pathogenesis have been hampered by the lack of reliable and efficient cell culture systems. A full-length consensus genome was cloned from viral RNA isolated from an infected human liver and used to construct subgenomic selectable replicons. Upon transfection into a human hepatoma cell line, these RNAs were found to replicate to high levels, permitting metabolic radiolabeling of viral RNA and proteins. This work defines the structure of HCV replicons funct…
"Table 8" of "Measurements of e+ e- ---> K+ K- eta, K+ K- pi0 and K0(s) K+- pi-+ cross- sections using initial state radiation events"
2008
Isoscalar (I=0) component of the cross section for E+ E- --> K2*(1430) K with statistical errors only.
A short screener is valid for assessing mediterranean diet adherence among older spanish men and women
2011
6 páginas.
Stop codon insertion restores the particle formation ability of hepatitis B virus core-hantavirus nucleocapsid protein fusions.
2003
In recent years, epitopes of various origin have been inserted into the core protein of hepatitis B virus (HBc), allowing the formation of chimeric HBc particles. Although the C-terminus of a C-terminally truncated HBc (HBcΔ) tolerates the insertion of extended foreign sequences, the insertion capacity is still a limiting factor for the construction of multivalent vaccines. Previously, we described a new system to generate HBcΔ mosaic particles based on a read-through mechanism in an <i>Escherichia coli</i> suppressor strain [J Gen Virol 1997;78:2049–2053]. Those mosaic particles allowed the insertion of a 114-amino acid (aa)-long segment of a Puumala hantavirus (PUUV) nucleocap…
Characterization of cell lines carrying self-replicating hepatitis C virus RNAs.
2001
ABSTRACT Subgenomic selectable RNAs of the hepatitis C virus (HCV) have recently been shown to self-replicate to high levels in the human hepatoma cell line Huh-7 (V. Lohmann, F. Körner, J. O. Koch, U. Herian, L. Theilmann, and R. Bartenschlager, Science 285:110–113, 1999). Taking advantage of this cell culture system that allows analyses of the interplay between HCV replication and the host cell, in this study we characterized two replicon-harboring cell lines that have been cultivated for more than 1 year. During this time, we observed no signs of cytopathogenicity such as reduction of growth rates or ultrastructural changes. High levels of HCV RNAs were preserved in cells passaged under…
Sequences in the 5′ Nontranslated Region of Hepatitis C Virus Required for RNA Replication
2001
ABSTRACT Sequences in the 5′ and 3′ termini of plus-strand RNA viruses harbor cis -acting elements important for efficient translation and replication. In case of the hepatitis C virus (HCV), a plus-strand RNA virus of the family Flaviviridae , a 341-nucleotide-long nontranslated region (NTR) is located at the 5′ end of the genome. This sequence contains an internal ribosome entry site (IRES) that is located downstream of an about 40-nucleotide-long sequence of unknown function. By using our recently developed HCV replicon system, we mapped and characterized the sequences in the 5′ NTR required for RNA replication. We show that deletions introduced into the 5′ terminal 40 nucleotides abolis…
Interferon-γ inhibits replication of subgenomic and genomic hepatitis C virus RNAs
2002
Persistent infection with hepatitis C virus (HCV) is a major cause of chronic hepatitis, liver cirrhosis, and hepatocellular carcinoma. All treatments known so far rely on the antiviral activity of interferon alfa (IFN-alpha) that is given alone or in combination with ribavirin. Unfortunately, only a fraction of the patients clear the virus during therapy and for those who do not respond there is currently no alternative treatment. Selectable subgenomic HCV RNAs (replicons) have been recently used to investigate the effect of IFN-alpha on HCV replication. However, it has not yet been analyzed whether other cytokines also play a role in the innate immune response against HCV. Here we show th…
Spectral analysis of the low-mass X-ray pulsar 4U 1822-371: Reflection component in a high-inclination system
2021
Context. The X-ray source 4U 1822-371 is an eclipsing low-mass X-ray binary and X-ray pulsar, hosting a NS that shows periodic pulsations in the X-ray band with a period of 0.59 s. The inclination angle of the system is so high (80–85°) that in principle, it should be hard to observe both the direct thermal emission of the central object and the reflection component of the spectrum because they are hidden by the outer edge of the accretion disc. Despite the number of studies carried out on this source, many aspects such as the geometry of the system, its luminosity, and its spectral features are still debated. Aims. Assuming that the source accretes at the Eddington limit, the analysis perf…
Detailed study of the X-ray and optical/UV orbital ephemeris of X1822-371
2011
Recent studies of the optical/UV and X-ray ephemerides of X1822-371 have found some discrepancies in the value of the orbital period derivative. Because of the importance of this value in constraining the system evolution, we comprehensively analyse all the available optical/UV/X eclipse times of this source to investigate the origin of these discrepancies. We collected all previously published X-ray eclipse times from 1977 to 2008, to which we added the eclipse time observed by Suzaku in 2006. This point is very important to cover the time gap between the last RXTE eclipse time (taken in 2003) and the most recent Chandra eclipse time (taken in 2008). Similarly we collected the optical/UV e…
Persistent and Transient Replication of Full-Length Hepatitis C Virus Genomes in Cell Culture
2002
ABSTRACT The recently developed subgenomic hepatitis C virus (HCV) replicons were limited by the fact that the sequence encoding the structural proteins was missing. Therefore, important information about a possible influence of these proteins on replication and pathogenesis and about the mechanism of virus formation could not be obtained. Taking advantage of three cell culture-adaptive mutations that enhance RNA replication synergistically, we generated selectable full-length HCV genomes that amplify to high levels in the human hepatoma cell line Huh-7 and can be stably propagated for more than 6 months. The structural proteins are efficiently expressed, with the viral glycoproteins E1 and…