Search results for "2A"
showing 10 items of 282 documents
Self-adjuvanting C18 lipid vinil sulfone-PP2A vaccine: study of the induced immunomodulation against
2017
Despite the importance of the adjuvant in the immunization process, very few adjuvants merge with the antigens in vaccines. A synthetic self-adjuvant oleic-vinyl sulfone (OVS) linked to the catalytic region of recombinant serine/threonine phosphatase 2A from the nematode Angiostrongylus costaricensis (rPP2A) was used for intranasal immunization in mice previously infected with Trichuris muris. The animal intranasal immunization with rPP2A-OVS showed a reduction of 99.01% in the number of the nematode eggs and 97.90% in adult. The immunohistochemical analysis of the intestinal sections showed that in immunized animals with lipopeptide the mucus was significantly higher than in the other expe…
Down-regulation of early sea urchin histone H2A gene relies on cis regulative sequences located in the 5' and 3' regions and including the enhancer b…
2004
The tandem repeated sea urchin alpha-histone genes are developmentally regulated by gene-specific promoter elements. Coordinate transcription of the five genes begins after meiotic maturation of the oocyte, continues through cleavage, and reaches its maximum at morula stage, after which these genes are shut off and maintained in a silenced state for the life cycle of the animal. Although cis regulative sequences affecting the timing and the level of expression of these genes have been characterized, much less is known about the mechanism of their repression. Here we report the results of a functional analysis that allowed the identification of the sequence elements needed for the silencing …
Determination of major human cytochrome P450s activities in 96-well plates using liquid chromatography tandem mass spectrometry.
2007
At the early stage of drug discovery, thousands of new chemical entities (NCEs) may be screened before a single candidate can be identified for development. Evaluation of the effect of NCEs on human CYP450 enzyme activities is a key issue in pharmaceutical development as it may explain inter-subject variability, drug-drug interactions, non-linear pharmacokinetics and toxic effects. A liquid chromatography tandem mass spectrometry (HPLC-MS/MS) method has been developed for the fast and routine analysis of major human CYP450s enzyme activities (CYP1A2, CYP2A6, CYP2B6, CYP2C9, CYP2C19, CYP2D6, CYP2E1 and CYP3A4) in primary hepatocyte cell cultures. The high sensitivity and selectivity of mass …
Multiplicative loops of 2-dimensional topological quasifields
2015
We determine the algebraic structure of the multiplicative loops for locally compact $2$-dimensional topological connected quasifields. In particular, our attention turns to multiplicative loops which have either a normal subloop of positive dimension or which contain a $1$-dimensional compact subgroup. In the last section we determine explicitly the quasifields which coordinatize locally compact translation planes of dimension $4$ admitting an at least $7$-dimensional Lie group as collineation group.
A Function Algebra Providing New Mergelyan Type Theorems in Several Complex Variables
2019
For compact sets $K\subset \mathbb C^{d}$, we introduce a subalgebra $A_{D}(K)$ of $A(K)$, which allows us to obtain Mergelyan type theorems for products of planar compact sets as well as for graphs of functions.
Influences of histone deacetylase inhibitors and resveratrol on DNA repair and chromatin compaction
2013
Accessibility of DNA is a prerequisite for both DNA damage and repair. Therefore, the chromatin structure is expected to have major impact on both processes, with opposite consequences for the stability of the genome. To analyse the influence of chromatin compaction on the generation and repair of various types of DNA modifications, we modulated the global chromatin structure of AS52 Chinese hamster ovary cells and HeLa cells by treatment with either histone deacetylase inhibitors or resveratrol and measured the repair kinetics of (i) pyrimidine dimers induced by ultraviolet B, (ii) oxidised purines generated by photosensitisation and (iii) single-strand breaks induced by H2O2, using an alk…
Histone carbonylation occurs in proliferating cells
2012
12 páginas, 10 figuras (que no es encuentran en este documento, se pueden ver en: http://www.sciencedirect.com/science/article/pii/S0891584912000664)
In Vitro Assessment of the Genotoxic Hazard of Novel Hydroxamic Acid- and Benzamide-Type Histone Deacetylase Inhibitors (HDACi)
2020
Histone deacetylase inhibitors (HDACi) are already approved for the therapy of leukemias. Since they are also emerging candidate compounds for the treatment of non-malignant diseases, HDACi with a wide therapeutic window and low hazard potential are desirable. Here, we investigated a panel of 12 novel hydroxamic acid- and benzamide-type HDACi employing non-malignant V79 hamster cells as toxicology guideline-conform in vitro model. HDACi causing a &ge
(177)Lu-labelled macrocyclic bisphosphonates for targeting bone metastasis in cancer treatment.
2015
Background Metastatic bone lesion is a common syndrome of many cancer diseases in an advanced state. The major symptom is severe pain, spinal cord compression, and pathological fracture, associated with an obvious morbidity. Common treatments including systemic application of bisphosphonate drugs aim on pain reduction and on improving the quality of life of the patient. Particularly, patients with multiple metastatic lesions benefit from bone-targeting therapeutic radiopharmaceuticals. Agents utilizing beta-emitting radionuclides in routine clinical praxis are, for example, [89Sr]SrCl2 and [153Sm]Sm-EDTMP. No-carrier-added (n.c.a.) 177Lu is remarkably suitable for an application in this sco…
PSD2 direktīvas ieviešana bankās
2020
2019. gada 14.septembrī Eiropas Savienībā stājās spēkā otrā Maksājumu pakalpojumu direktīva, pazīstama kā PSD2 (Payment Service Directive 2). Latvijā tās nosacījumi iestrādāti Maksājumu pakalpojumu un elektroniskās naudas likumā. Darba mērķis ir aprakstīt PSD2 pakalpojumu ieviešanu bankās, tas ir, dot aprakstu bankām par prasībām un dažādām komponentēm, kas ir jāimplementē, lai ieviestu PSD2 direktīvu, piemēram, kā, PSD2 lietotne, atļauju apstrāde, SSL/TLS sertifikātu apstrāde un trešās puses pakalpojumu sniedzēja servisi. Darbā tiek analizētas specifikācijas, kas apraksta PSD2 lietotnes interfeisu, noskaidrojot obligātās prasības un būtiskākās atšķirības. Darbā tiek piedāvāts risinājuma ap…