Search results for "322"

showing 10 items of 170 documents

UV-induced cross-linking of proteins to plasmid pBR322 containing 8-azidoadenine 2′-deoxyribonucleotides

1988

Abstract An efficient method of cross-linking DNA to protein is described. The method is based on the incorporation of photoactive 8-azidoadenine 2′-deoxyribonucleotides into DNA. We have found that 8-N 3 dATP is a substrate for E. coli DNA polymerase I and that 8-N 3 dATP can be incorporated into plasmid pBR322 by nick-translation. Subsequently we were able to cross-link a set of different proteins to 8-azido-2′-deoxyadenosine-containing pBR322 by UV irradiation (366 nm). No DNA-protein photocross-linking was observed under the same conditions when the non-photoactive plasmid pBR322 was used.

Ultraviolet RaysDNA polymeraseDNA polymerase IIUltraviolet irradiationBiophysicsAzidoadeninePlasmid pBR322BiochemistryHistonesDeoxyadenine NucleotidesPlasmidStructural BiologyEscherichia coliGeneticsNick translationMolecular BiologyPlasmid preparationDNA clampNick-translationbiologyDNA-protein cross-linkCell BiologyDNA Polymerase IPBR322Cross-Linking ReagentsBiochemistrybiology.proteinDNA polymerase IPlasmidsFEBS Letters
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Det er jo bare et spill"? : en studie om dataspill og selvopplevelse

2012

Masteroppgave i samfunnskommunikasjon- Universitetet i Agder, 2012

VDP::Social science: 200::Library and information science: 320::Information politics: 322
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E-deltakelse, sosiale medier og politikk : partienes tilstedeværelse i sosiale medier

2009

Masteroppgave i informasjonssystemer- Universitetet i Agder 2009 På grunn av sviktende oppslutning om partier og valg er e-deltakelse er et svært aktuelt tema. E-deltakelsesprosjekter har ofte problemer med få deltakere, derfor ønsker man å undersøke hvordan sosiale medier kan brukes for e-deltakelse. Valgkamp på internett har vært et tema siden stortingsvalget i 2001. I 2008 hadde presidentkandidat Barack Obama stor suksess med å bruke sosiale medier i den amerikanske presidentvalgkampen. Av den grunn fokuseres det på deltakelsesformen ”valgkamp”. Studiens problemstilling er ”Hva er partienes strategi for, og erfaringer med, sosiale medier i innledningen til stortingsvalgkampen 2009, og hv…

VDP::Social science: 200::Library and information science: 320::Information politics: 322IS501
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Visuell retorikk hos ideelle hjelpeorganisasjoner på internet

2012

Masteroppgave i samfunnskommunikasjon- Universitetet i Agder 2012

VDP::Social science: 200::Library and information science: 320::Information politics: 322KOM 500
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Porsgrunn kommune "likes" Facebook : en studie om bruk av sosiale medier i kommunal sektor

2012

Masteroppgave i samfunnskommunikasjon- Universitetet i Agder 2012 Facebook has until recently been used by private individuals rather than governmental organizations. Figures from Difi (Agency for Public Management and eGovernment) says that Facebook has 2,5 millions Norwegian members per 01.2012. This study will explore what are the factors that come into play, when Porsgrunn municipality adopt Facebook to improve inhabitants' dialogue, their cooperation and democratic development. Current research shows that the participation of inhabitants, is very important for the municipality if they want to succeed in their use of Facebook. Even more importantly is the municipality´s will to listen, …

VDP::Social science: 200::Library and information science: 320::Information politics: 322KOM 500ComputerApplications_GENERAL
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Social media as public sphere : an exploratory study of online political communication in Norway

2013

Doctoral dissertation, Faculty of Economics and Social Sciences, University of Agder, Kristiansand 2013 This thesis presents an exploratory study of the use of social media in the Norwegian public sector, and contributes to the understanding of how social media can be applied to improve deliberation. The study is situated within the eParticipation research field. The motivation for the study is the reported challenges facing democracy, in the form of lower voter turnout, decline in political party membership, and a general lessening of interest for public issues and deliberation. Deliberation is seen as essential for democracy, and promoting deliberation could well lead to an increase in vo…

VDP::Social science: 200::Library and information science: 320::Information politics: 322VDP::Social science: 200::Political science and organizational theory: 240VDP::Social science: 200::Library and information science: 320::Information and communication systems: 321
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Louis Le Roy traducteur et commentateur des Politiques ou comment tirer Aristote de « l’ombre scolastique »

2017

International audience; No abtract

[SHS.LITT] Humanities and Social Sciences/Literature[SHS.LITT]Humanities and Social Sciences/Literature[ SHS.LITT ] Humanities and Social Sciences/LiteratureHistoriographie -- EuropeAristote (0384-0322 av. J.-C.)
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Contro l’uso della nullita ` parziale in chiave di conformazione del contratto

2020

.Il saggio analizza la tendenza giurisprudenziale a servirsi della nullita ` parziale ex art. 1419 c.c. per realizzare interventi sul contratto animati dall’obiettivo di ridefinirne il contenuto in funzione di riequilibrio dei relativi termini. Il caso piu ` eclatante registrato di recente e ` rappresentato dalla sentenza a Sezioni unite del 2018 in materia di contratti di assicurazione della responsabilita ` civile con copertura claims made, nella quale si e ` assistito all’abbandono del grimaldello della meritevolezza degli interessi di cui all’art. 1322 c.c. a vantaggio di un uso altamente censurabile della categoria della causa del contratto che sfocia in una declaratoria di nullita ` p…

partial nullitySettore IUS/01 - Diritto Privatoclaims made coverage"meritevolezza" of the interestart. 1322 Italian Civil Codecivil liability insurance contracts
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Contratos de permuta financiera ante el impuesto sobre el valor añadido

2021

El estudio de la tributación en el impuesto sobre el valor añadido de los contratos de permuta financiera, exige el previo conocimiento de estos derivados financieros. Para ello acudimos a la jurisprudencia del Tribunal de Justicia de la Unión europea y a las nociones de la Contabilidad internacional

swaps 322 353:CIENCIAS JURÍDICAS [UNESCO]permutas financieras.UNESCO::CIENCIAS JURÍDICAS2070-8157 22082 Revista Boliviana de Derecho 565487 2021 31 7730061 Contratos de permuta financiera ante el impuesto sobre el valor añadido Miguel Canutoexige el previo conocimiento de estos derivados financieros. Para ello acudimos a la jurisprudencia del Tribunal de Justicia de la Unión europea y a las nociones de la Contabilidad internacional IVAVATEnrique de El estudio de la tributación en el impuesto sobre el valor añadido de los contratos de permuta financiera
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Joan B. Llinares, una reflexión filosófica a través de la literatura

2020

una reflexión filosófica a través de la literatura PerisUNESCO::CIENCIAS DE LAS ARTES Y LAS LETRAS1575-2259 2322 Pasajes: Revista de pensamiento contemporáneo 570906 2020 61 7829573 Joan B. LlinaresManuel 147 152:CIENCIAS DE LAS ARTES Y LAS LETRAS [UNESCO]Revista de pensamiento contemporáneo 570906 2020 61 7829573 Joan B. Llinares [1575-2259 2322 Pasajes]
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