Search results for "38"

showing 10 items of 1730 documents

Cadmium effects on p38/MAPK isoforms in MDA-MB231 breast cancer cells

2009

Emerging evidence seems to indicate that the heavy metal cadmium (Cd) is able to regulate gene expression, drastically affecting the pattern of transcriptional activity in normal and pathological eukaryotic cells, also affecting intracellular signalization events. Human p38 is a family of mitogen-activated protein kinases consisting of four isoforms (alpha, beta, gamma and delta) which mediate signal transduction cascades controlling several aspects of cell physiology. In this study we examined whether exposure of MDA-MB231 tumor cells from the human breast to Cd may exert some effect on p38 isoform expression and accumulation, as well as on p38 activation. Employing a combination of prolif…

Transcriptional ActivationGene isoformCadmium SB203580 p38 isoforms p38 activation Gene expressionCell SurvivalPyridinesp38 mitogen-activated protein kinasesBreast NeoplasmsBiologyp38 Mitogen-Activated Protein KinasesGene Expression Regulation EnzymologicGeneral Biochemistry Genetics and Molecular BiologyBiomaterialsStructure-Activity RelationshipGene expressionTumor Cells CulturedHumansSettore BIO/06 - Anatomia Comparata E CitologiaCell ProliferationRegulation of gene expressionDose-Response Relationship DrugKinaseImidazolesMetals and AlloysMolecular biologyCell biologyIsoenzymesCell cultureDrug Screening Assays AntitumorSignal transductionGeneral Agricultural and Biological SciencesIntracellularCadmiumBioMetals
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The Streptococcal Exotoxin Streptolysin O Activates Mast Cells To Produce Tumor Necrosis Factor Alpha by p38 Mitogen-Activated Protein Kinase- and Pr…

2003

ABSTRACTStreptolysin O (SLO), a major virulence factor of pyogenic streptococci, binds to cholesterol in the membranes of eukaryotic cells and oligomerizes to form large transmembrane pores. While high toxin doses are rapidly cytocidal, low doses are tolerated because a limited number of lesions can be resealed. Here, we report that at sublethal doses, SLO activates primary murine bone marrow-derived mast cells to degranulate and to rapidly induce or enhance the production of several cytokine mRNAs, including tumor necrosis factor alpha (TNF-α). Mast cell-derived TNF-α plays an important protective role in murine models of acute inflammation, and the production of this cytokine was analyzed…

Transcriptional ActivationImmunologyBiologyp38 Mitogen-Activated Protein KinasesMicrobiologyMiceBacterial ProteinsmedicineAnimalsASK1Mast CellsRNA MessengerProtein kinase AProtein Kinase CProtein kinase CMice Inbred BALB CDose-Response Relationship DrugTumor Necrosis Factor-alphaMast cellMolecular PathogenesisProtein kinase RMolecular biologyInterleukin 33Infectious Diseasesmedicine.anatomical_structureStreptolysinsParasitologyTumor necrosis factor alphaStreptolysinMitogen-Activated Protein KinasesInfection and Immunity
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Development of S/MAR minicircles for enhanced and persistent transgene expression in the mouse liver.

2010

We have previously described the development of a scaffold/matrix attachment region (S/MAR) episomal vector system for in vivo application and demonstrated its utility to sustain transgene expression in the mouse liver for at least 6 months following a single administration. Subsequently, we observed that transgene expression is sustained for the lifetime of the animal. The level of expression, however, does drop appreciably over time. We hypothesised that by eliminating the bacterial components in our vectors, we could improve their performance since bacterial sequences have been shown to be responsible for the immunotoxicity of the vector and the silencing of its expression when applied i…

TransgeneGenetic VectorsEnzyme-Linked Immunosorbent AssayBiologyMinicircleMolecular biologyPolymerase Chain ReactionScaffold/matrix attachment region (S/MAR) – Minicircle – Plasmid – Non-viral – Gene therapy – Liver – Hydrodynamic deliveryBlotting SouthernMicePlasmidSettore MED/38 - Pediatria Generale E SpecialisticaLiverIn vivoCell Line TumorDrug DiscoveryGene expressionMolecular MedicineGene silencingAnimalsHumansExpression cassetteTransgenesScaffold/matrix attachment regionGenetics (clinical)Journal of molecular medicine (Berlin, Germany)
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MTHFR C677T homozygous as risk factor for complications after OLT for cryptogenic cirrhosis

2006

TransplantationPediatricsmedicine.medical_specialtySettore MED/38 - Pediatria Generale E Specialisticabusiness.industryMTHFR C677T OLT Cryptogenic cirrhosis.Cryptogenic cirrhosisMthfr c677tMedicineRisk factorbusinessClinical Transplantation
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Olivier Messiaen: el músico rítmico

2014

Trinidad 241 252UNESCO::CIENCIAS DE LAS ARTES Y LAS LETRAS:CIENCIAS DE LAS ARTES Y LAS LETRAS [UNESCO]0211-5808 9678 Archivo de arte valenciano 385455 2014 95 4940220 Olivier Messiaen: el músico rítmico Lull Nayael músico rítmico Lull Naya [0211-5808 9678 Archivo de arte valenciano 385455 2014 95 4940220 Olivier Messiaen]
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TROMBOSI VENOSA PROFONDA DA ESTROPROGESTINICI IN TETRASOMIA X

2009

Trombosi venosa profonda Tetrasomia X EstroprogestiniciSettore MED/38 - Pediatria Generale E Specialistica
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MMP-10/stromelysin-2 promotes invasion of head and neck cancer.

2011

BackgroundPeriostin, IFN-induced transmembrane protein 1 (IFITM1) and Wingless-type MMTV integration site family, member 5B (Wnt-5b) were previously identified as the invasion promoted genes of head and neck squamous cell carcinoma (HNSCC) by comparing the gene expression profiles between parent and a highly invasive clone. We have previously reported that Periostin and IFITM1 promoted the invasion of HNSCC cells. Here we demonstrated that Wnt-5b overexpression promoted the invasion of HNSCC cells. Moreover, stromelysin-2 (matrix metalloproteinase-10; MMP-10) was identified as a common up-regulated gene among Periostin, IFITM1 and Wnt-5b overexpressing HNSCC cells by using microarray data s…

Tumor PhysiologyClone (cell biology)p38 Mitogen-Activated Protein KinasesMetastasisMetastasisMolecular Cell BiologyBasic Cancer ResearchNeoplasm MetastasisRegulation of gene expressionGene knockdownMultidisciplinaryHead and Neck cancerQRTransfectionHead and Neck TumorsExtracellular MatrixUp-RegulationGene Expression Regulation NeoplasticOncologyHead and Neck NeoplasmsGene Knockdown TechniquesCarcinoma Squamous CellMedicineResearch ArticleScience490Oral MedicineBiologyPeriostinHead and Neck Squamous Cell CarcinomaMatrix Metalloproteinase 10stomatognathic systemSettore MED/28 - Malattie OdontostomatologicheCell Line TumormedicineCancer Detection and Diagnosisotorhinolaryngologic diseasesHumansNeoplasm Invasiveness490BiologyExtracellular Matrix AdhesionsProtein Kinase InhibitorsneoplasmsMicroarray analysis techniquesCancers and Neoplasmsmedicine.diseaseMolecular biologyHead and neck squamous-cell carcinomaAntigens DifferentiationWnt Proteinsstomatognathic diseasesCancer researchCell Adhesion MoleculesPLoS ONE
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Acidic Environment Leads to ROS-Induced MAPK Signaling in Cancer Cells

2011

Tumor micromilieu often shows pronounced acidosis forcing cells to adapt their phenotype towards enhanced tumorigenesis induced by altered cellular signalling and transcriptional regulation. In the presents study mechanisms and potential consequences of the crosstalk between extra- and intracellular pH (pH(e), pH(i)) and mitogen-activated-protein-kinases (ERK1/2, p38) was analyzed. Data were obtained mainly in AT1 R-3327 prostate carcinoma cells, but the principle importance was confirmed in 5 other cell types. Extracellular acidosis leads to a rapid and sustained decrease of pH(i) in parallel to p38 phosphorylation in all cell types and to ERK1/2 phosphorylation in 3 of 6 cell types. Furth…

Tumor PhysiologyIntracellular Spacelcsh:MedicineSignal transductionERK signaling cascadeMolecular cell biologyNeoplasmsBasic Cancer ResearchTumor MicroenvironmentSignaling in Cellular ProcessesPhosphorylationCyclic AMP Response Element-Binding ProteinCreb Signalinglcsh:ScienceCellular Stress ResponsesMultidisciplinaryKinaseMechanisms of Signal TransductionSignaling cascadesHydrogen-Ion ConcentrationProtein-Tyrosine KinasesCell biologyOncologyMedicinePhosphorylationMitogen-Activated Protein KinasesSodium-Potassium-Exchanging ATPaseIntracellularResearch ArticleCell SurvivalMAP Kinase Signaling Systemp38 mitogen-activated protein kinasesIntracellular pHBiologyCREBModels BiologicalCell GrowthDogsCell Line TumorAnimalsHumansProtein Kinase InhibitorsBiologyPI3K/AKT/mTOR pathwaylcsh:RRatsEnzyme ActivationCancer cellbiology.proteinlcsh:QExtracellular SpaceReactive Oxygen SpeciesAcidsPLoS ONE
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CCL3 and CCL4, the Major Chemokines Produced by CD38+ Chronic Lymphocytic Leukemia Cells, Facilitate Microenvironmental Interactions of Neoplastic Ce…

2008

Abstract CD38, a negative prognostic marker for patients with CLL, has been demonstrated to be a key molecule in the interactions occurring in the context of tumor microenvironment, mediating both survival and migratory signals for CLL cells. By taking advantage of gene expression profiling studies (GEP) comparing 11 CD38pos (CD38>30%) and 15 CD38neg (CD38<10%) CLLs, we identified as over-expressed in CD38pos CLL cells: i) genes for the two C-C chemokines CCL3 and CCL4 (median-log difference, MLD-CCL3= 3.5; MLD-CCL4=4.4); real-time quantitative PCR (RTQ-PCR) of selected cases confirmed GEP results; ii) the gene for CD49d (MLD=4.4); a high correlation between CD38 and CD49d pro…

Tumor microenvironmentChemokineChronic lymphocytic leukemiamedicine.medical_treatmentImmunologyContext (language use)Cell BiologyHematologyBiologyCD38medicine.diseaseBiochemistryBeta ChemokineGene expression profilingCytokineimmune system diseaseshemic and lymphatic diseasesImmunologymedicinebiology.proteinBlood
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TERATOMA IMMATURO EXTRACRANICO: Risultati dello Studio Cooperativo AIEOP "TCG-91".

1998

I Teratomi Immatuti sono tumori costituiti da tessuto non maligno ma con potenzialità evolutiva maligna. Gli Autori presentano i dati dello Studio Cooperativo "TCG-91".

Tumori Germinali Pediatrici Teratomi ImmaturiSettore MED/38 - Pediatria Generale E SpecialisticaSettore MED/20 - Chirurgia Pediatrica E Infantile
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