Search results for "3T"

showing 10 items of 171 documents

Recombinant mussel protein Pvfp-5β: A potential tissue bioadhesive

2019

During their lifecycle, many marine organisms rely on natural adhesives to attach to wet surfaces for movement and self-defence in aqueous tidal environments. Adhesive proteins from mussels are biocompatible and elicit only minimal immune responses in humans. Therefore these proteins have received increased attention for their potential applications in medicine, biomaterials and biotechnology. The Asian green mussel Perna viridis secretes several byssal plaque proteins, molecules that help anchor the mussel to surfaces. Among these proteins, protein-5β (Pvfp-5β) initiates interactions with the substrate, displacing interfacial water molecules before binding to the surface. Here, we establis…

0301 basic medicinemedicine.disease_causeBiochemistryepidermal growth factor (EGF)law.inventionMiceCell Movementlawbiophysicsstructural biologyrecombinantCells CulturedbiologyChemistryMarine proteinsAdhesionRecombinant ProteinsadhesionProtein Structure and FoldingRecombinant DNAadhesion proteinsbiomaterialsPernaCell SurvivalSurface PropertiesBioadhesivemussel03 medical and health sciencesmedicineAnimalsHumansMolecular BiologyEscherichia coliCell ProliferationTissue Engineering030102 biochemistry & molecular biologyProteinsCell BiologyMusselbiology.organism_classificationEGF-like motifs; Marine proteins; adhesion; adhesion proteins; biomaterials; biophysics; epidermal growth factor (EGF); structural biologyEGF-like motifs030104 developmental biologyStructural biologyCell cultureNIH 3T3 CellsBiophysicsTissue AdhesivesHeLa CellsPerna viridisJournal of Biological Chemistry
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"Table 4" of "Linear and non-linear flow modes in Pb-Pb collisions at $\sqrt{s_{\rm NN}} =$ 2.76 TeV"

2019

Study of relationship between linear and non-linear modes in higher order anisotropic flow in Pb–Pb collisions at 2.76 TeV.

2760.0V5V2V3v22overV5V2V3Tv22GapPb Pb --> CHARGED X
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Genomic organization and promoter characterization of the gene encoding a putative endoplasmic reticulum chaperone, ERp29

2002

Abstract ERp29 is a soluble protein localized in the endoplasmic reticulum (ER) of eukaryotic cells, which is conserved in all mammalian species. The N-terminal domain of ERp29 displays sequence and structural similarity to the protein disulfide isomerase despite the lack of the characteristic double cysteine motif. Although the exact function of ERp29 is not yet known, it was hypothesized that it may facilitate folding and/or export of secretory proteins in/from the ER. ERp29 is induced by ER stress, i.e. accumulation of unfolded proteins in the ER. To gain an insight into the mechanisms regulating ERp29 expression we have cloned and characterized the rat ERp29 gene and studied in details …

5' Flanking RegionRecombinant Fusion ProteinsMolecular Sequence DataCHO CellsBiologyCell LineMiceCricetinaeSequence Homology Nucleic AcidGene expressionTumor Cells CulturedGeneticsAnimalsHumansRNA MessengerLuciferasesPromoter Regions GeneticProtein disulfide-isomeraseGeneHeat-Shock ProteinsPhylogenyBase SequenceGene Expression ProfilingEndoplasmic reticulumPromoter3T3 CellsDNAExonsSequence Analysis DNAGeneral MedicineMolecular biologyIntronsRatsHousekeeping geneSecretory proteinGenesUnfolded protein responseFemaleTranscription Initiation SiteSequence AlignmentHeLa CellsGene
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Hypertrophy and ER Stress Induced by Palmitate Are Counteracted by Mango Peel and Seed Extracts in 3T3-L1 Adipocytes

2023

A diet rich in saturated fatty acids (FAs) has been correlated with metabolic dysfunction and ROS increase in the adipose tissue of obese subjects. Thus, reducing hypertrophy and oxidative stress in adipose tissue can represent a strategy to counteract obesity and obesity-related diseases. In this context, the present study showed how the peel and seed extracts of mango (Mangifera indica L.) reduced lipotoxicity induced by high doses of sodium palmitate (PA) in differentiated 3T3-L1 adipocytes. Mango peel (MPE) and mango seed (MSE) extracts significantly lowered PA-induced fat accumulation by reducing lipid droplet (LDs) and triacylglycerol (TAGs) content in adipocytes. We showed that MPE a…

AMPKsaturated fatty acidOrganic ChemistryGeneral MedicineNrf2CatalysisComputer Science ApplicationsInorganic Chemistrymango seed extractSettore BIO/10 - Biochimicamango peel extracts; mango seed extracts; saturated fatty acids; 3T3-L1 adipocytes; ER stress; AMPK; Nrf2ER strePhysical and Theoretical Chemistrymango peel extract3T3-L1 adipocyteMolecular BiologySpectroscopyInternational Journal of Molecular Sciences; Volume 24; Issue 6; Pages: 5419
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Novel mutations of the MET proto-oncogene in papillary renal carcinomas.

1999

Hereditary papillary renal carcinoma (HPRC) is characterized by multiple, bilateral papillary renal carcinomas. Previously, we demonstrated missense mutations in the tyrosine kinase domain of the MET proto-oncogene in HPRC and a subset of sporadic papillary renal carcinomas. In this study, we screened a large panel of sporadic papillary renal carcinomas and various solid tumors for mutations in the MET proto-oncogene. Summarizing these and previous results, mutations of the MET proto-oncogene were detected in 17/129 sporadic papillary renal carcinomas but not in other solid tumors. We detected five novel missense mutations; three of five mutations were located in the ATP-binding region of t…

AdenomaModels MolecularCancer ResearchProtein ConformationDNA Mutational AnalysisMolecular Sequence DataHereditary Papillary Renal Cell CarcinomaBiologymedicine.disease_causeTransfectionProto-Oncogene MasReceptor tyrosine kinaseMiceAdenosine TriphosphateNeoplastic Syndromes HereditaryProto-OncogenesGeneticsCarcinomamedicineMissense mutationAnimalsHumansPoint MutationAmino Acid SequencePhosphorylationCodonMolecular BiologyKidneyMutationBinding SitesSequence Homology Amino AcidPoint mutation3T3 CellsDNA NeoplasmProto-Oncogene Proteins c-metmedicine.diseaseCarcinoma PapillaryKidney NeoplasmsNeoplasm Proteinsmedicine.anatomical_structureCell Transformation NeoplasticCancer researchbiology.proteinMutagenesis Site-DirectedTyrosine kinaseProtein Processing Post-TranslationalSequence AlignmentOncogene
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Lymphoma cell apoptosis in the liver induced by distant murine cytomegalovirus infection.

2006

ABSTRACTCytomegalovirus (CMV) poses a threat to the therapy of hematopoietic malignancies by hematopoietic stem cell transplantation, but efficient reconstitution of antiviral immunity prevents CMV organ disease. Tumor relapse originating from a minimal residual leukemia poses another threat. Although a combination of risk factors was supposed to enhance the incidence and severity of transplantation-associated disease, a murine model of a liver-adapted B-cell lymphoma has previously shown a survival benefit and tumor growth inhibition by nonlethal subcutaneous infection with murine CMV. Here we have investigated the underlying antitumoral mechanism. Virus replication proved to be required, …

Adoptive cell transferProgrammed cell deathMuromegalovirusLymphoma B-CellCD30Lymphomamedicine.medical_treatmentImmunologyApoptosisHematopoietic stem cell transplantationBiologyCD8-Positive T-Lymphocytesmedicine.disease_causeLymphoma T-CellMicrobiologyVirusHerpesviridaeMiceVirologyCell Line TumormedicineAnimalsPoint MutationBone Marrow TransplantationMice Inbred BALB CHerpesviridae Infectionsmedicine.diseaseVirologyAdoptive TransferLymphomaLeukemiaLiverMice Inbred DBAInsect ScienceNIH 3T3 CellsPathogenesis and ImmunityFemaleJournal of virology
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Inhibition of skin inflammation by baicalin ultradeformable vesicles.

2016

The topical efficacy of baicalin, a natural flavonoid isolated from Scutellaria baicalensis Georgi, which has several beneficial properties, such as antioxidative, antiviral, anti-inflammatory and antiproliferative, is hindered by its poor aqueous solubility and low skin permeability. Therefore, its incorporation into appropriate phospholipid vesicles could be a useful tool to improve its local activity. To this purpose, baicalin at increasing concentrations up to saturation, was incorporated in ultradeformable vesicles, which were small in size (∼67nm), monodispersed (PI<0.19) and biocompatible, regardless of the concentration of baicalin, as confirmed by in vitro studies using fibroblasts…

AdultCell SurvivalSkin AbsorptionPharmaceutical ScienceDermatitis02 engineering and technologyPharmacologyAdministration Cutaneous03 medical and health scienceschemistry.chemical_compoundMice0302 clinical medicineIn vivomedicineAnimalsHumansDexamethasoneTransdermalFlavonoidsDrug CarriersbiologyEpidermis (botany)Vesicle3T3 CellsMiddle Aged021001 nanoscience & nanotechnologybiology.organism_classificationIn vitrochemistry030220 oncology & carcinogenesisScutellaria baicalensisFemale0210 nano-technologyBaicalinmedicine.drugInternational journal of pharmaceutics
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Analysis of the gastrin-releasing peptide receptor gene in Italian patients with autism spectrum disorders

2008

The gastrin-releasing peptide receptor (GRPR) was implicated for the first time in the pathogenesis of Autism spectrum disorders (ASD) by Ishikawa-Brush et al. [Ishikawa-Brush et al. (1997): Hum Mol Genet 6: 1241-1250]. Since this original observation, only one association study [Marui et al. (2004): Brain Dev 26: 5-7] has further investigated, though unsuccessfully, the involvement of the GRPR gene in ASD. With the aim of contributing further information to this topic we have sequenced the entire coding region and the intron/exon junctions of the GRPR gene in 149 Italian autistic patients. The results of this study led to the identification of four novel point mutations, two of which, that…

AdultMalemedicine.medical_specialtyBALB 3T3 CellsAdolescentDNA Mutational AnalysisPopulationRett syndromeBiologyMiceCellular and Molecular NeuroscienceExonSettore BIO/13 - Biologia ApplicataInternal medicineGastrin-releasing peptideChlorocebus aethiopsmedicineGastrin-releasing peptide receptorAnimalsHumansPoint MutationAutistic DisorderChildautism gastrin-releasing peptide receptor signal transductionG-protein-coupled receptor association studyeducationGeneGenetics (clinical)AgedGeneticseducation.field_of_studyPoint mutationMiddle Agedmedicine.diseasePedigreeReceptors BombesinDevelopmental disorderPsychiatry and Mental healthEndocrinologyItalyCase-Control StudiesCOS CellsFemaleAmerican Journal of Medical Genetics Part B: Neuropsychiatric Genetics
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A new PCSK9 gene promoter variant affects gene expression and causes autosomal dominant hypercholesterolemia.

2008

Autosomal dominant hypercholesterolemia (ADH) is a genetic disorder characterized by increased low-density lipoprotein (LDL)-cholesterol levels, leading to high risk of premature cardiovascular disease. More than 900 mutations in LDL receptor, six in APOB and 10 in PCSK9 have been identified as a cause of the disease in different populations. All known mutations in PCSK9 causing hypercholesterolemia produce an increase in the enzymatic activity of this protease. Up to now, there are data about the implication of PCSK9 in ADH in a low number of populations, not including a Spanish population.The objective of the study was to study the prevalence of PCSK9 mutations in ADH Spanish population.W…

Adultmedicine.medical_specialtyApolipoprotein BEndocrinology Diabetes and MetabolismClinical BiochemistryGene ExpressionTransfectionBiochemistryPolymorphism Single NucleotideHyperlipoproteinemia Type IIPCSK9 GeneMiceEndocrinologyGene FrequencyInternal medicinemedicineAnimalsHumansPromoter Regions GeneticAllele frequencyGeneCells CulturedGeneticsbiologyBase SequencePCSK9Biochemistry (medical)Serine EndopeptidasesGenetic disorderHyperlipoproteinemia Type IIaMiddle Agedmedicine.diseaseEndocrinologySpainCase-Control StudiesLDL receptorbiology.proteinNIH 3T3 Cellslipids (amino acids peptides and proteins)Mutant ProteinsProprotein ConvertasesProprotein Convertase 9The Journal of clinical endocrinology and metabolism
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Fonti, suggestioni e intersezioni classiche, tardoantiche e medievali nell’«Alda» di Guglielmo di Blois

2019

In questo saggio vengono studiate le fonti classiche, tardoantiche e medievali dell’«Alda», commedia elegiaca latina di Guglielmo di Blois (scritta verso il 1169-1170). In particolare, dopo una presentazione generale dell’opera e una rassegna critica degli studi e delle edizioni, ci si sofferma sulle suggestioni attinte a Terenzio, Virgilio, Orazio, Ovidio, Stazio, all’«Aegritudo Perdicae», Massimiano, Boezio, Ildeberto di Lavardin, ad alcune commedie elegiache del sec. XII (il «Geta» e l’«Aulularia» di Vitale di Blois, il «Pamphilus», la «Lidia» di Arnolfo d’Orléans) e alla tradizione novellistica “orientale” («Mischle Sendebar»). This essay is founded on the classical, late and medieval s…

AldaVirgiltradizioni classiche tardoantiche e medievali.StatiuStazioSettore L-FIL-LET/08 - Letteratura Latina Medievale E UmanisticaHoraceGuglielmo di BloiWilliam of BloiOvidioAegritudo PerdicaeMassimianonovellistica medievaleTerenzioclassical late antique and medieval traditions.TerenceOraziocommedia elegiaca latina del XII e XIII secoloVirgilioIldeberto of Lavardinmedieval storieIldeberto di LavardinOvidLatin elegiac comedy of the 12th and 13th centurieMaximian
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