Search results for "40"
showing 10 items of 17345 documents
Fig virus-free production and survival rate improvement using meristem tip culture techinique associated with the encapsulation technology
2018
Three Mediterranean F. carica genotypes, i.e. cultivars Palazzo, Bifera nera and Catalanisca, initially infected by Fig leaf mottle-associated virus 1 (FLMaV-1), Fig leaf mottle-associated virus 2 (FLMaV-2), Fig mild mottling-associated virus (FMMaV), Fig mosaic virus (FMV), Fig latent virus 1 (FLV-1), Fig Badnavirus 1 (FBV-1) and Fig fleck-associated virus (FFkaV), were subjected to the sanitation technique via Meristem Tip (0.3-0.5 mm in size) Culture (MTC), also associated with the encapsulation technique (MTC-SS), in order to produce virus-free plant material. Encapsulation was tested to overcome the very low survival and regeneration rates, due to the small propagule size. Encouraging …
Seasonal Variations in Biochemical Composition of the ClamDosinia corrugatein Relation to the Reproductive Cycle and Environmental Conditions
2016
ABSTRACT Seasonal variations in biochemical composition of the clam Dosinia corrugate were investigated from August 2013 until July 2014 in Zhuanghe Bay in relation to environmental conditions and reproductive cycle. Separate biochemical analyses of the mantle, adductor muscle, foot, and gonad-visceral mass in each sex were undertaken. Spawning took place once a year from July to August and the massive spawning occurred in August with the highest water temperature and chlorophyll a levels. Utilization of glycogen took place during the spawning period, whereas protein was biosynthesized as the mature gametes formed. The glycogen value increased during the resting stage (autumn—winter). The r…
Plant-Based Protein Hydrolysate Improves Salinity Tolerance in Hemp: Agronomical and Physiological Aspects
2021
Hemp (Cannabis sativa L.) is a multipurpose plant attracting increasing interest as a source for the production of natural fibers, paper, bio-building material and food. In this research we studied the agronomical performance of Cannabis sativa cv. Eletta Campana irrigated with saline water. Under those conditions, we tested the effect of protein hydrolysate (PH) biostimulant application in overcoming and/or balancing deleterious salinity effects. The results of the diverse treatments were also investigated at the physiological level, focusing on photosynthesis by means of a chlorophyll a fluorescence technique, which give an insight into the plant primary photochemical reactions. Four sali…
Optical characteristics of greenhouse plastic films affect yield and some quality traits of spinach (Spinacia oleracea L.) subjected to different nit…
2021
Light and nitrogen strongly affect the growth, yield, and quality of food crops, with greater importance in green leafy vegetables for their tendency to accumulate nitrate in leaves. The purpose of this research was to explore the effect of two greenhouse films (Film A and B) on yield, and quality of spinach grown under different nitrogen regimes (not fertilized—N0%
Improved Extraction Efficiency of Antioxidant Bioactive Compounds from Tetraselmis chuii and Phaedoactylum tricornutum Using Pulsed Electric Fields
2020
Pulsed electric fields (PEF) is a promising technology that allows the selective extraction of high-added value compounds by electroporation. Thus, PEF provides numerous opportunities for the energy efficient isolation of valuable microalgal bioactive substances (i.e., pigments and polyphenols). The efficiency of PEF-assisted extraction combined with aqueous or dimethyl sulfoxide (DMSO) solvents in recovering pigments and polyphenols from microalgae Tetraselmis chuii (T. chuii) and Phaeodactylum tricornutum (P. tricornutum) was evaluated. Two PEF treatments were applied: (1 kV/cm/400 pulses, 3 kV/cm/45 pulses), with a specific energy input of 100 kJ/kg. The total antioxidant capacity (TAC) …
Stirring-assisted dead-end ultrafiltration for protein and polyphenol recovery from purple sweet potato juices: Filtration behavior investigation and…
2016
The potential of stirring assisted dead-end ultrafiltration (UF) to recover polyphenols from purple sweet potato (PSP) juices was evaluated. For this purpose, the effects of rotation speed (200–600 rpm), transmembrane pressure (TMP) (0.2–0.4 MPa), and membrane molecular weight cut-off (30–100 kDa) were investigated with a lab-scale filtration module. The results showed that the highest protein removal (86%) and polyphenol selectivity (12.2) were both obtained under higher rotation speed (600 rpm). Moreover, filtration flux was more important at 600 rpm due to the anti-fouling effect of shear rate generated by the rotation. The results also revealed that under TMP of 0.4 MPa, the cake resist…
Development of an UHPLC-Q-Orbitrap HRMS method for simultaneous determination of mycotoxins and isoflavones in soy-based burgers
2019
Abstract Soy-based burgers, an option of meatless hamburger-style, are basically made with soy protein, wholegrains and veggies. However, some ingredients, such as cereals and soybean, are particularly susceptible to fungal contamination. Therefore, a fast and simple procedure for the simultaneous determination of isoflavones (n=12) and mycotoxins (n=21) in soy-based burgers was developed throughout an acetonitrile-based extraction and UHPLC-Q-Exactive Orbitrap HRMS determination for the first time. The procedure was properly validated in terms of linearity, accuracy (recovery range from 78 to 108%) and precision (RSD
Chromatographic separation and partial identification of glycosidically bound volatile components of fruit
1990
Abstract Synthetic monoterpenic and aromatic β- d -glucosides and β- d -rutinosides were separated by Fractogel TSK HW-40 S chromatography according to their molecular size and interactions occurring between the aglycone moiety and the gel matrix. Under these conditions terpenyl rutinosides were eluted before the homologous glucoside derivatives. In the two classes, monoglucosides and rutinosides, aromatic glycosidically bound components had lower retention times than the corresponding terpenyl components. The use of over-pressure layer chromatography (OPLC) allowed the separation of glucoside and rutinoside derivatives, and in these two classes aromatic and several monoterpene compounds we…
Identification of Casein Phosphopeptides in β-casein and Commercial Hydrolysed Casein by Mass Spectrometry
2006
Casein phosphopeptides (CPPs) in commercial hydrolysed casein (CE90CPP) and in β-CN (β-CN) after simulated gastrointestinal digestion (gastric stage pepsin, pH =2, 37°C 2h) and intestinal stage (pancreatic-bile extract, pH =5.2, 37°C 2h) were sequenced by on-line reversed-phase high performance liquid chromatography coupled to electrospray ionisation tandem mass spectrometry (RP-HPLC-ESIMS/MS). In β-CN digest five peptides that contained four to five phosphate groups and the cluster sequence SpSpSpEE (residues 17-21) were identified. All CPPs with one exception β-CN(1-24)4P, had the protein fragment β-CN(1-25)4P, which is one of the main CPPs produced in vivo digestion of casein and the re…
Multiple paths to cold tolerance: the role of environmental cues, morphological traits and the circadian clock gene vrille
2021
AbstractBackgroundTracing the association between insect cold tolerance and latitudinally and locally varying environmental conditions, as well as key morphological traits and molecular mechanisms, is essential for understanding the processes involved in adaptation. We explored these issues in two closely-related species, Drosophila montana and Drosophila flavomontana, originating from diverse climatic locations across several latitudes on the coastal and mountainous regions of North America. We also investigated the association between sequence variation in one of the key circadian clock genes, vrille, and cold tolerance in both species. Finally, we studied the impact of vrille on fly cold…