Search results for "43"

showing 10 items of 1438 documents

Role of calcineurin in Ca2+-induced release of catecholamines and neuropeptides

1998

Neurotransmission requires rapid docking, fusion, and recycling of neurotransmitter vesicles. Several of the proteins involved in this complex Ca2+-regulated mechanism have been identified as substrates for protein kinases and phosphatases, e.g., the synapsins, synaptotagmin, rabphilin3A, synaptobrevin, munc18, MARCKS, dynamin I, and B-50/GAP-43. So far most attention has focused on the role of kinases in the release processes, but recent evidence indicates that phosphatases may be as important. Therefore, we investigated the role of the Ca2+/calmodulin-dependent protein phosphatase calcineurin in exocytosis and subsequent vesicle recycling. Calcineurin-neutralizing antibodies, which blocke…

MaleSynaptobrevinCYCLOSPORINE-APhosphataseCalcineurin InhibitorsB-50 GAP-43Biologydynamin IBiochemistryBRAIN NERVE-TERMINALSExocytosisSynaptotagmin 1SincalidephosphataseGeneeskundeCellular and Molecular NeuroscienceNorepinephrineBacterial ProteinsPERMEATED SYNAPTOSOMESAnimalsratNEUROTRANSMITTER RELEASEMARCKSEnzyme InhibitorsRats WistarPROTEIN-KINASE-CDynaminCalcineurinTRANSMITTER RELEASEDYNAMIN-ISynapsinPhosphoric Monoester HydrolasesRatsINDUCED NORADRENALINE RELEASECalcineurinBiochemistryImmunoglobulin GStreptolysinsCalciumexocytosisCALMODULIN-BINDINGSynaptosomes
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Immunoproteasome LMP2 60HH Variant Alters MBP Epitope Generation and Reduces the Risk to Develop Multiple Sclerosis in Italian Female Population

2010

BackgroundAlbeit several studies pointed out the pivotal role that CD4+T cells have in Multiple Sclerosis, the CD8+ T cells involvement in the pathology is still in its early phases of investigation. Proteasome degradation is the key step in the production of MHC class I-restricted epitopes and therefore its activity could be an important element in the activation and regulation of autoreactive CD8+ T cells in Multiple Sclerosis.Methodology/principal findingsImmunoproteasomes and PA28-alphabeta regulator are present in MS affected brain area and accumulated in plaques. They are expressed in cell types supposed to be involved in MS development such as neurons, endothelial cells, oligodendroc…

MaleT cells proteasomes multiple sclerosis parietal lobeMuscle ProteinsImmunoproteasomeEpitopeEpitopesGene FrequencyRisk FactorsCytotoxic T cellFunding: This work was financed in part by the grant Giovani Ricercatori 2007 from Italian Ministry of Health to MM DG and FMB by a grant from the European Commission Integrated Project PROTEOMAGE (FP6) to CF by the finalized projects of Fondazione Italiana Sclerosi Multipla (FISM) cod. 2003/R26 and BioPharmaNet to CF and 2002/R/40 and 2005/R/10 2008/R/11 (Genoa) to SD'A by the University of Bologna (FRO) to MPF by the Regione Piemonte (Ricerca Sanitaria Finalizzata Project and Ricerca Sanitaria Applicata-CIPE Project) to SD'A by Associazione Amici del Centro Dino Ferrari and IRCCS Ospedale Maggiore Policlinico Milano to DG and by the grants Sonderforschungsbereich (SFB-507 SFB-421) to PMK and US the grants TR43 and Neurocure to PMK. MM benefited from the A.V. Humboldt PostDoc fellowship. The funders had no role in study design data collection and analysis decision to publish or preparation of the manuscript.MultidisciplinaryMicrogliaQRBrainMiddle AgedImmunohistochemistryCysteine EndopeptidasesOligodendrogliamedicine.anatomical_structureItalyImmunoproteasome; multiple sclerosis; italian populationmultiple sclerosiImmunology/Antigen Processing and RecognitionMedicineFemaleMicrogliaNeuroscience/Neurobiology of Disease and RegenerationResearch ArticleProtein BindingAdultProteasome Endopeptidase ComplexMultiple SclerosisGenotypeScienceMolecular Sequence DataImmunology/AutoimmunityBiologySex FactorsMHC class IHLA-A2 AntigenmedicineHumansAmino Acid SequenceAlleleHLA-A AntigensMultiple sclerosisMacrophagesMyelin Basic Proteinmedicine.diseaseMyelin basic proteinImmunologybiology.proteinitalian populationCD8PLoS ONE
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A novel LC–MS/MS analytical method for detection of articaine and mepivacaine in blood and its application to a preliminary pharmacokinetic study

2020

Local anaesthetics (LAs) are commonly used in surgery, especially in dentistry. They cause a transitory inhibition of nerve signal due to the blockade of the voltage-gated sodium channels. LAs are administrated alone or with vasoconstriction agents, such as adrenaline. Toxicity of LAs is associated to neurological and cardiovascular alterations. Tachycardia, arrhythmia, tremors, tonic-clonic seizure and respiratory depression (at high doses) are the main symptoms of intoxication by LAs. Lidocaine, articaine and mepivacaine are among the most used anaesthetics. This study aimed to fully validated a new method for the simultaneous detection of articaine and mepivacaine in whole blood. Sample …

MaleTachycardiaLidocaineLiquid-Liquid ExtractionClinical BiochemistryMepivacainePharmaceutical ScienceCarticaineArticaine01 natural sciencesAnalytical ChemistryPharmacokineticsSettore MED/43 - Medicina LegaleLC–MS/MSTandem Mass SpectrometryArticaine Mepivacaine Local anaesthetics LC–MS/MS BloodbloodDrug DiscoverymedicineHumansAnesthetics LocalSpectroscopyarticaine; blood; LC–MS/MS; local anaesthetics; mepivacaineWhole blood010405 organic chemistryChemistry010401 analytical chemistrySelected reaction monitoringReproducibility of Results0104 chemical sciencesarticaineAnesthesiaToxicityFemalelocal anaestheticsmedicine.symptomChromatography Liquidmedicine.drugmepivacaine
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Targeted profiling of circulating and hepatic bile acids in human, mouse, and rat using a UPLC-MRM-MS-validated method

2012

Bile acids (BAs) are a group of chemically related steroids recognized as regulatory molecules whose profiles can change in different physio-pathological situations. We have developed a sensitive, fast, and reproducible ultraperformance liquid chromatography/multiple reaction monitoring/mass spectrometry method to determine the tissue and sera BA profiles in different species (human, rat, and mouse) by quantifying 31 major and minor BA species in a single 21-min run. The method has been validated according to FDA guidelines, and it generally provides good results in terms of intra- and interday precision (less than 8.6% and 16.0%, respectively), accuracy (relative error measurement between …

MaleTaurocholic AcidQD415-436BiologyMass spectrometryBiochemistryHigh-performance liquid chromatographyMass SpectrometryBile Acids and SaltsMicechemistry.chemical_compoundEndocrinologyMetabolomicsSpecies Specificitytargeted analysisLipidomicsMethodsAnimalsHumansChromatographySelected reaction monitoringCell BiologyMetabolismTaurocholic acidmetabolomicsHepatic bileRatsLiverBiochemistrychemistrylipidomicsChromatography LiquidJournal of Lipid Research
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The role of combined techniques of scintigraphy and SPECT/CT in the diagnosis of primary hyperparathyroidism

2019

Abstract Rationale: Primary hyperparathyroidism, usually as a result of a hyperfunctioning parathyroid gland, represents more than 90% cases of patients evaluated for hypercalcemia. Combined techniques of preoperative scintigraphy and SPECT/CT serve as a successful minimally-invasive parathyroidectomy. This recent imaging method provides four-dimensional functional images with advanced contrast resolution which greatly facilitates preoperative localization of parathyroid adenomas. Patient concerns: We presented the case of a male Caucasian patient, aged 67 years, who was investigated for hypercalcemia. Increased levels of parathormone, cervical ultrasonography without pathological changes, …

MaleTechnetium Tc 99m Sestamibi4300Single Photon Emission Computed Tomography Computed Tomographyprimary hyperparatyroidismSPECT/CTHyperparathyroidism PrimaryMultimodal ImagingParathyroid NeoplasmsscintigraphyHumansClinical Case ReportRadiopharmaceuticalsRadionuclide ImagingResearch ArticleAgedMedicine
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Efficacy of a blended low-intensity internet-delivered psychological programme in patients with multimorbidity in primary care: study protocol for a …

2019

BackgroundThe World Health Organization (WHO) has included comorbidity between depression and a chronic disease among the 10 leading global health priorities. Although there is a high prevalence of multimorbidity, health care systems are mainly designed for the management of individual diseases. Given the difficulty in delivering face-to-face psychological treatments, alternative models of treatment delivery have been proposed, emphasizing the role of technologies such as the Internet. The aim of this study is to assess the efficacy in Primary Care (PC) of a blended low-intensity psychological intervention applied using information and communication technologies (ICTs) for the treatment of …

MaleTecnología de la informaciónhumanosPsychological interventiondepresiónEnsayo clínico controlado aleatoriolaw.invention:Organisms::Eukaryota::Animals::Chordata::Vertebrates::Mammals::Primates::Haplorhini::Catarrhini::Hominidae::Humans [Medical Subject Headings]dolor lumbarStudy Protocol0302 clinical medicineRandomized controlled triallawlcsh:PsychiatryDepresiónHealth careGlobal healthMedicine030212 general & internal medicineRandomized Controlled Trials as Topic:Analytical Diagnostic and Therapeutic Techniques and Equipment::Investigative Techniques::Epidemiologic Methods::Epidemiologic Study Characteristics as Topic::Clinical Trials as Topic::Controlled Clinical Trials as Topic::Randomized Controlled Trials as Topic [Medical Subject Headings]:Analytical Diagnostic and Therapeutic Techniques and Equipment::Investigative Techniques::Evaluation Studies as Topic::Clinical Trials as Topic::Controlled Clinical Trials as Topic::Randomized Controlled Trials as Topic [Medical Subject Headings]:Diseases::Pathological Conditions Signs and Symptoms::Pathologic Processes::Disease Attributes::Chronic Disease [Medical Subject Headings]Depressionensayos clínicos controlados aleatorizados como asuntoMultimorbilidadadultoPrimary careLow back pain:Health Care::Health Services Administration::Patient Care Management::Comprehensive Health Care::Primary Health Care [Medical Subject Headings]Psychiatry and Mental healthdiabetes mellitus:Information Science::Information Science::Computing Methodologies::Computer Systems::Computer Communication Networks::Internet [Medical Subject Headings]:Diseases::Pathological Conditions Signs and Symptoms::Signs and Symptoms::Pain::Back Pain::Low Back Pain [Medical Subject Headings]Femalemedicine.symptomAdultmedicine.medical_specialtyenfermedad crónicalcsh:RC435-571:Check Tags::Male [Medical Subject Headings]03 medical and health sciencesInformation and communication technologies (ICTs)Atención primaria de saludIntervention (counseling)Humans:Persons::Persons::Age Groups::Adult [Medical Subject Headings]:Geographical Locations::Geographic Locations::Europe::Spain [Medical Subject Headings]InternetPrimary Health Carebusiness.industry:Diseases::Endocrine System Diseases::Diabetes Mellitus::Diabetes Mellitus Type 2 [Medical Subject Headings]Multimorbidity:Health Care::Health Care Facilities Manpower and Services::Health Personnel::Physicians::Physicians Primary Care [Medical Subject Headings]medicine.diseaseComorbidity030227 psychiatryClinical trial:Check Tags::Female [Medical Subject Headings]Diabetes Mellitus Type 2SpainChronic DiseasePhysical therapyRandomized controlled trial (RCT)business:Psychiatry and Psychology::Behavior and Behavior Mechanisms::Behavior::Behavioral Symptoms::Depression [Medical Subject Headings]Low Back PainBMC psychiatry
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Fatty liver in familial hypobetalipoproteinemia: triglyceride assembly into VLDL particles is affected by the extent of hepatic steatosis

2003

Familial hypobetalipoproteinemia (FHBL) subjects may develop fatty liver. Liver fat was assessed in 21 FHBL with six different apolipoprotein B (apoB) truncations (apoB-4 to apoB-89) and 14 controls by magnetic resonance spectroscopy (MRS). Liver fat percentages were 16.7 +/- 11.5 and 3.3 +/- 2.9 (mean +/- SD) (P = 0.001). Liver fat percentage was positively correlated with body mass index, waist circumference, and areas under the insulin curves of 2 h glucose tolerance tests, suggesting that obesity may affect the severity of liver fat accumulation in both groups. Despite 5-fold differences in liver fat percentage, mean values for obesity and insulin indexes were similar. Thus, for similar…

MaleVery low-density lipoproteinSettore MED/09 - Medicina InternaApolipoprotein Bmedicine.medical_treatmentPalmitic AcidLipoproteins VLDLSeverity of Illness IndexTriglyceridevery low density lipoprotein assemblyBiochemistryBody Mass IndexMagnetic resonence spectroscopy; Nonalcoholic fatty liver; Nonesterified fatty acids; Very low density lipoprotein assembly; Adult; Body Mass Index; Dietary Fats; Fatty Liver; Female; Glucose Tolerance Test; Humans; Hypobetalipoproteinemias; Insulin Resistance; Lipoproteins VLDL; Liver Diseases; Male; Middle Aged; Obesity; Palmitic Acids; Severity of Illness Index; Triglycerides; EndocrinologyHypobetalipoproteinemiaschemistry.chemical_compoundEndocrinologyDietary FatGlucose tolerance testmedicine.diagnostic_testbiologyChemistryLiver DiseasesLiver DiseaseFatty liverMiddle AgedFemalemagnetic resonence spectroscopyHumanAdultmedicine.medical_specialtyPalmitic Acidsnonesterified fatty acidsQD415-436Internal medicinemedicinenonalcoholic fatty liverHumansObesityTriglyceridesTriglycerideInsulinNonesterified fatty acidCell BiologyGlucose Tolerance Testmedicine.diseaseDietary FatsFatty LiverEndocrinologybiology.proteinHypobetalipoproteinemiaInsulin ResistanceSteatosisHypobetalipoproteinemiaJournal of Lipid Research
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Moors and Saracens in Europe: estimating the medieval North African male legacy in southern Europe.

2009

To investigate the male genetic legacy of the Arab rule in southern Europe during medieval times, we focused on specific Northwest African haplogroups and identified evolutionary close STR-defined haplotypes in Iberia, Sicily and the Italian peninsula. Our results point to a higher recent Northwest African contribution in Iberia and Sicily in agreement with historical data, southern Italian regions known to have experienced long-term Arab presence also show an enrichment of Northwest African types. The forensic and genomic implications of these findings are discussed.

MaleY chromosome north africa medieval legacyPopulationShort ReportNorth africaHaplogroupZoological sciencesEvolution MolecularMoorsAfrica NorthernPeninsulaSettore BIO/13 - Biologia ApplicataHumansgeneticseducationGenetics (clinical)education.field_of_studygeography.geographical_feature_categoryChromosomes Human YGeographyEvolution (zoology)social sciencesSettore MED/43 - MEDICINA LEGALEpopulationseye diseasesArabsEuropeGeographyGenetics PopulationHaplotypesAnthropologySaracenEthnologyNorth africangeographic locationsEuropean journal of human genetics : EJHG
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Farnesoid X receptor activation increases cholesteryl ester transfer protein expression in humans and transgenic mice

2013

International audience; Cholesteryl ester transfer protein (CETP) activity results in a proatherogenic lipoprotein profile. In cholestatic conditions, farnesoid X receptor (FXR) signaling by bile acids (BA) is activated and plasma HDL cholesterol (HDL-C) levels are low. This study tested the hypothesis that FXR-mediated induction of CETP contributes to this phenotype. Patients with cholestasis and high plasma BA had lower HDL-C levels and higher plasma CETP activity and mass compared with matched controls with low plasma BA (each P < 0.01). BA feeding in APOE3*Leiden transgenic mice expressing the human CETP transgene controlled by its endogenous promoter increased cholesterol within apoB-c…

Male[SDV]Life Sciences [q-bio]Receptors Cytoplasmic and Nuclear030204 cardiovascular system & hematologyInbred C57BLBiochemistryTransgenicchemistry.chemical_compoundMice0302 clinical medicineEndocrinologyHigh-density lipoproteinLifeReceptorsnuclear receptorResearch ArticlesCells Cultured0303 health sciencesCulturedbiologyMiddle AgedUp-RegulationCytoplasmic and Nuclear/agonistslipids (amino acids peptides and proteins)FemaleEELS - Earth Environmental and Life SciencesMHR - Metabolic Health ResearchHealthy Livingmedicine.medical_specialtyTransgeneCellsMice TransgenicQD415-436macrophageReceptors Cytoplasmic and Nuclear/agonists03 medical and health sciencesDownregulation and upregulationInternal medicineCholesterylester transfer proteinmedicinehepatocyteFood and NutritionAnimalsHumans[SDV.BBM]Life Sciences [q-bio]/Biochemistry Molecular Biology030304 developmental biologyNutritionbile acidsCholesterolGene Expression ProfilingCell BiologyCholesterol Ester Transfer Proteinscarbohydrates (lipids)Mice Inbred C57BLlipoproteinsEndocrinologyNuclear receptorchemistrybiology.proteinFarnesoid X receptor[SDV.AEN]Life Sciences [q-bio]/Food and NutritionLipoproteinCholesterol Ester Transfer Proteins/genetics
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The association among interpersonal problems, binge behaviors, and self-esteem, in the assessment of obese individuals

2011

Abstract Objectives Although disordered eating has been assumed to be associated with interpersonal problems, there is a lack of research regarding the relationship between interpersonal problems and obesity. This study explored associations among self-esteem, binge behaviors, and interpersonal problems in obese individuals, by contrasting obese persons with overweight persons, and to investigate whether body mass index (BMI), binge behaviors, and self-esteem predict interpersonal problems in obese individuals. Methods A group of nonobese overweight people (n = 65; BMI range, 25-29.9 kg/m 2 ) and a group of obese people (n = 78; BMI >35 kg/m 2 ) were selected from 224 people attending a men…

Malelcsh:RC435-571Interpersonal communicationOverweightBody Mass IndexInterpersonal relationshipSurveys and Questionnaireslcsh:PsychiatrySettore M-PSI/08 - Psicologia ClinicamedicineBody ImageHumansInterpersonal RelationsObesityDisordered eatingBulimiaobesity interpersonal problems self-esteem binge eating disordermedicine.diseaseObesitySelf ConceptPsychiatry and Mental healthClinical PsychologyEating disordersRegression AnalysisFemalemedicine.symptomBinge Eating ScalePsychologyBody mass indexClinical psychology
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