Search results for "45"

Polychlorinated biphenyls, classified as either phenobarbital- or 3-methylcholanthrene-type inducers of cytochrome P-450, are both hepatic tumor prom…

1986

Abstract The cytochrome P -450 isozymes, cytochrome P -450 MC 1 and MC 2 , purified from rats treated with 3-methylcholanthrene (MC), were found by immunohistochemical staining to be strongly induced in the livers of rats treated with 3,3′, 4,4′-tetrachlorobiphenyl (TCBP), while the cytochrome P -450 isozymes, PB 1 and PB 2 , purified from the livers of rats treated with phenobarbital (PB), were shown to be induced in the livers of rats treated with 2,2′, 4,4′, 5,5′-hexachlorobiphenyl (HCBP). The latter compound also strongly induced NADPH-cytochrome P -450-reductase. Following induction, all 5 enzymes were located preferentially in the centrilobular and midzonal region of the liver acinus.…

FOXP2 gene and language impairment in schizophrenia: association and epigenetic studies

2010

Abstract Background Schizophrenia is considered a language related human specific disease. Previous studies have reported evidence of positive selection for schizophrenia-associated genes specific to the human lineage. FOXP2 shows two important features as a convincing candidate gene for schizophrenia vulnerability: FOXP2 is the first gene related to a language disorder, and it has been subject to positive selection in the human lineage. Methods Twenty-seven SNPs of FOXP2 were genotyped in a cohort of 293 patients with schizophrenia and 340 controls. We analyzed in particular the association with the poverty of speech and the intensity of auditory hallucinations. Potential expansion of thre…

Energy availability during training camp is associated with signs of overreaching and changes in performance in young female cross-country skiers

2021

Abstract Study aim: The aim of this study was to evaluate if young female skiers meet their energy and macronutrient requirements, and how energy availability (EA) and macronutrient intake affects their performance during an intensive training camp. Material and methods: 19 female cross-country skiers (age 16.7 ± 0.7) filled in 48-hour food and training logs during a 5-day training camp. Fasting concentrations of hemoglobin, leptin, triiodothyronine (T3), insulin, insulin-like growth factor 1 (IGF-1), and glucose were measured before (PRE) and after (POST) the camp. Blood lactate (LA), heart rate (HR) and rating of perceived exertion (RPE) from a submaximal treadmill running test, jump heig…

Human Embryonic Stem Cell Derived Hepatocyte-Like Cells as a Tool for In Vitro Hazard Assessment of Chemical Carcinogenicity

2011

Hepatocyte-like cells derived from the differentiation of human embryonic stem cells (hES-Hep) have potential to provide a human relevant in vitro test system in which to evaluate the carcinogenic hazard of chemicals. In this study, we have investigated this potential using a panel of 15 chemicals classified as noncarcinogens, genotoxic carcinogens, and nongenotoxic carcinogens and measured whole-genome transcriptome responses with gene expression microarrays. We applied an ANOVA model that identified 592 genes highly discriminative for the panel of chemicals. Supervised classification with these genes achieved a cross-validation accuracy of > 95%. Moreover, the expression of the response g…

Re-expression of C/EBP alpha induces CYP2B6, CYP2C9 and CYP2D6 genes in HepG2 cells.

1998

Cytochrome P450 (CYP) activity is very low or even absent in human hepatomas, a phenomenon that is accompanied by low levels of some liver transcription factors, notably C/EBP alpha. To investigate a possible link between this transcription factor and hepatic CYP expression, we have stably transfected HepG2 cells with a C/EBP alpha vector containing a Zn-inducible metallothionein promoter. Expression of functional C/EBP alpha up to liver levels concomitantly increased the mRNAs of several members of the CYP2 family (2B6, 2C9 and 2D6), suggesting that this transcription factor may play a relevant role in controlling the hepatic expression of CYP enzymes.

Toxicity of waste gasification bottom ash leachate

2011

Abstract Toxicity of waste gasification bottom ash leachate from landfill lysimeters (112 m3) was studied over three years. The leachate of grate incineration bottom ash from a parallel setup was used as reference material. Three aquatic organisms (bioluminescent bacteria, green algae and water flea) were used to study acute toxicity. In addition, an ethoxyresorufin-O-deethylase (EROD) assay was performed with mouse hepatoma cells to indicate the presence of organic contaminants. Concentrations of 14 elements and 15 PAH compounds were determined to characterise leachate. Gasification ash leachate had a high pH (9.2–12.4) and assays with and without pH adjustment to neutral were used. Gasifi…

Can Hepatoma Cell Lines be Re-differentiated to be Used in Drug Metabolism Studies?

2013

Knowledge of metabolism, enzymes so far involved, and potential enzyme-inhibiting or enzyme-inducing properties of new compounds is a key issue in drug development. Primary cultured hepatocytes, cytochrome P450 (CYP)-engineered cells and hepatoma cell lines are currently being used for this purpose, but only primary cultures can produce a metabolic profile of a drug similar to that found in vivo and can respond to inducers. Because of their limited accessibility, alternatives to replace human hepatocytes are currently being explored, including the immortalisation of hepatocytes by using different strategies (i.e. SV40 T-large antigen, conditionally immortalised hepatocytes, transfection wi…

Exercise intervention protocol in children and young adults with cerebral palsy : the effects of strength, flexibility and gait training on physical …

2021

Abstract Background Individuals with cerebral palsy (CP) have problems in everyday tasks such as walking and climbing stairs due to a combination of neuromuscular impairments such as spasticity, muscle weakness, reduced joint flexibility and poor coordination. Development of evidence-based interventions are in pivotal role in the development of better targeted rehabilitation of CP, and thus in maintaining their motor function and wellbeing. Our aim is to investigate the efficacy of an individually tailored, multifaceted exercise intervention (EXECP) in children and young adults with CP. EXECP is composed of strength, flexibility and gait training. Furthermore, this study aims to verify the …

Una opinión sobre la muerte de don Carlos de Austria, príncipe de Asturias, primogénito del rey Felipe II : causas que la motivaron y retrato de algu…

Teresa y Luis, Luis y Teresa. Dos santos en tiempos recios

2016

Resumen: El presente articulo analiza la relacion entre dos grandes santos espanoles de la Contrarreforma Catolica, Teresa de Jesus y el dominico fray Luis Bertran Palabras clave: Teresa de Jesus, Luis Bertran, Santos, Contrarreforma, Carmelitas Descalzos, Dominicos, Siglo XVI Abstract: The present article analyses the relation between two big Spanish saints of the Catholic Counter-reformation, Teresa de Jesus and the Dominican monk Luis Bertran Keywords : Teresa de Jesus, Luis Bertran, Saints, Counter-reformation, Discalced Carmelite, Dominicans, 16th century