Search results for "56"
showing 10 items of 1818 documents
18F-Radiolabeling, Preliminary Evaluation of Folate-pHPMA Conjugates via PET
2014
The synthesis of a 10.5 kDa and a 52.5 kDa polymer, based on pHPMA functionalized with tyramine for (18) F-labeling and a folate derivative as targeting moiety, is reported. FCS studies are conducted using Oregon Green-labeled conjugates. No aggregation is observed for the 10.5 kDa conjugate, but strong aggregation for the 52.5 kDa conjugate. In vivo studies are conducted using Walker-256 mammary carcinoma model to determine body distribution as function of size and especially targeting unit. These in vivo studies show a higher short time (2 h) accumulation for both conjugates in the tumor than for untargeted pHPMA, confirmed by blockade studies. The 10.5 kDa polymer accumulates with 0.46% …
in vitro biological evaluation of folate-functionalized block copolymer micelles for selective anti-cancer drug delivery.
2008
The main objective of this study was to evaluate the ability of folic acid-functionalized diblock copolymer micelles to improve the delivery and uptake of two poorly water-soluble anti-tumor drugs, tamoxifen and paclitaxel, to cancer cells through folate receptor targeting. The diblock copolymer used in this study comprised a hydrophilic poly[2-(methacryloyloxy)ethyl phosphorylcholine] (MPC) block, carrying at the chain end the folate targeting moiety, and a pH-sensitive hydrophobic poly[2-(diisopropylamino)ethyl methacrylate] (DPA) block (FA-MPC-DPA). The drug-loading capacities of tamoxifen- and paclitaxel-loaded micelles were determined by high performance liquid chromatography and the m…
Cytotoxic effects of polyphenols from waste-water of extra virgin olive oil on human HL60 and K562 cell lines.
2009
The behavior of solutions of a parametric weighted (p, q)-laplacian equation
2021
<abstract><p>We study the behavior of solutions for the parametric equation</p> <p><disp-formula> <label/> <tex-math id="FE1"> \begin{document}$ -\Delta_{p}^{a_1} u(z)-\Delta_{q}^{a_2} u(z) = \lambda |u(z)|^{q-2} u(z)+f(z,u(z)) \quad \mbox{in } \Omega,\, \lambda &gt;0, $\end{document} </tex-math></disp-formula></p> <p>under Dirichlet condition, where $ \Omega \subseteq \mathbb{R}^N $ is a bounded domain with a $ C^2 $-boundary $ \partial \Omega $, $ a_1, a_2 \in L^\infty(\Omega) $ with $ a_1(z), a_2(z) &gt; 0 $ for a.a. $ z \in \Omega $, $ p, q \in (1, \infty) $ and $ \Delta_{p}^{a_1}, \Delta_{q}^{a_2} $ are weighted …
Diet matters, particularly in pregnancy – Results from MoBa studies of maternal diet and pregnancy outcomes
2014
Published version of an article in the journal: Norsk Epidemiologi. Also available from the publisher at: http://www.ntnu.no/ojs/index.php/norepid/article/view/1805/1802 Open Access Awareness that maternal diet may influence the outcome of pregnancy as well as the long-term health of mother and child has increased in recent years. A new food frequency questionnaire (FFQ) was developed and validated specifically for the Norwegian Mother and Child Cohort Study (MoBa). The MoBa FFQ is a semi-quantitative tool which covers the average intake of food, beverages and dietary supplements during the first 4 to 5 months of pregnancy. It includes questions about intakes of 255 foods and dishes and was…
A new approach to predict the fish fillet shelf-life in presence of natural preservative agents
2017
Three data sets concerning the behaviour of spoilage flora of fillets treated with natural preservative substances (NPS) were used to construct a new kind of mathematical predictive model. This model, unlike other ones, allows expressing the antibacterial effect of the NPS separately from the prediction of the growth rate. This approach, based on the introduction of a parameter into the predictive primary model, produced a good fitting of observed data and allowed characterising quantitatively the increase of shelf-life of fillets.
Cell cooperation in coelomocyte cytotoxic activity of Paracentrotus lividus coelomocytes
2007
The coelomic fluid from the sea urchin Paracentrotus lividus contains several coelomocyte types including amoebocytes and uncoloured spherulocytes involved in immune defences. In the present paper, we show a Ca(2+)-dependent cytotoxic activity for the unfractionated coelomocytes assayed in vitro, with rabbit erythrocytes and the K562 tumour cell line. In a plaque-forming assay, whole coelomocyte preparations as well as density gradient separated coelomocyte populations revealed that cell populations enriched in uncoloured spherulocytes, exerted high cytotoxic activity by releasing lysins in the presence of amoebocytes. This cooperative effect could be dependent on soluble factors released b…
Programmed cell death (PCD) associated with the stilbene motif of arotinoids: discovery of novel apoptosis inducer agents possessing activity on mult…
2000
Considering that the stereochemistry of the C9-C10 alkenyl portion of natural 9-cis-RA, as the one of the olefinic moiety of the previously described isoxazole retinoid 4, seems of particular importance for their apoptotic activity, we prepared a novel class of TTNPB analogues bearing both the cis or trans configuration of the alkenyl portion. The compounds were evaluated in vitro for their cytotoxic and apoptotic activities. We discovered that the cis-TTNPB 9c possesses apoptotic activity comparable with that of the retinoid 4. Moreover, the amino arotinoid 16c showed potent apoptotic activity in HL60 promyelocytic leukemia cells. Interestingly, 16c proved to be a particularly potent apopt…
Comparative Proteome Profiling and Functional Analysis of Chronic Myelogenous Leukemia Cell Lines
2007
The aim of the present study was the molecular profiling of different Ph+ chronic myelogenous leukemia (CML) cell lines (LAMA84, K562, and KCL22) by a proteomic approach. By employing two-dimensional gel electrophoresis combined with mass spectrometry analysis, we have identified 191 protein spots corresponding to 142 different proteins. Among these, 63% were cancer-related proteins and 74% were described for the first time in leukemia cells. Multivariate analysis highlighted significant differences in the global proteomic profile of the three CML cell lines. In particular, the detailed analysis of 35 differentially expressed proteins revealed that LAMA84 cells preferentially expressed prot…
Criterios relevantes e innovadores del Consejo de Transparencia de la Comunidad Valenciana
2019
espanolLa Comunidad Valenciana ha destacado en los ultimos anos en materia de transparencia. Sin embargo, su Consejo de Transparencia por diversos motivos ha quedado bastante oculto en su intensa labor. El estudio, analiza y expone las resoluciones y criterios mas innovadores y relevantes de este organo en sus cientos de resoluciones habidas desde inicios de 2016. Se considera que muchos de tales criterios deben ser cuanto menos conocidos y, en su caso, incorporarse o, cuanto menos, incitar al debate por especialistas y otros Consejos y autoridades de transparencia estatal o autonomicas. EnglishThe Valencian Community has highlighted in the last years in transparency. However, the intense w…