Search results for "572"

Tandem repeats lead to sequence assembly errors and impose multi-level challenges for genome and protein databases

2019

AbstractThe widespread occurrence of repetitive stretches of DNA in genomes of organisms across the tree of life imposes fundamental challenges for sequencing, genome assembly, and automated annotation of genes and proteins. This multi-level problem can lead to errors in genome and protein databases that are often not recognized or acknowledged. As a consequence, end users working with sequences with repetitive regions are faced with ‘ready-to-use’ deposited data whose trustworthiness is difficult to determine, let alone to quantify. Here, we provide a review of the problems associated with tandem repeat sequences that originate from different stages during the sequencing-assembly-annotatio…

Exploring organ-specific features of fibrogenesis using murine precision-cut tissue slices

2019

Fibrosis is the hallmark of pathologic tissue remodelling in most chronic diseases. Despite advances in our understanding of the mechanisms of fibrosis, it remains uncured. Fibrogenic processes share conserved core cellular and molecular pathways across organs. In this study, we aimed to elucidate shared and organ-specific features of fibrosis using murine precision-cut tissue slices (PCTS) prepared from small intestine, liver and kidneys. PCTS displayed substantial differences in their baseline gene expression profiles: 70% of the extracellular matrix (ECM)-related genes were differentially expressed across the organs. Culture for 48 h induced significant changes in ECM regulation and trig…

Desafíos del sindicalismo italiano ante el trabajo sindical con trabajadores ?contingentes? en Amazon

2021

Rompiendo el silencio. Infancias amenazadas

2018

Signal transduction pathways of membrane expression of proteinase 3 (PR‐3) in human endothelial cells

1997

At present, the exact mechanism of the pathogenic effect of anti-PR-3 antibodies remains unknown. Interaction of anti-neutrophil cytoplasmic antibodies (ANCAs) with human umbilical vein endothelial cells (HUVECs) may play a key role. Recently we were able to show that ANCAs recognize their target antigen, PR-3, translocated into the membrane of HUVECs. The objective of this study was to investigate regulation, i.e. signal transduction pathways, of PR-3 expression in endothelial cells. HUVECs were isolated according to the method of Jaffe et al. and cultured under standard conditions. A cyto-enzyme-linked immunosorbent assay (ELISA) with unfixed cells was performed. Membrane-expressed PR-3 w…

Projekteilla parempaa laatua yliopistoihin?

2005

Markkinatalousvetoisen kulttuurin soveltaminen yliopistoihin ei ole ongelmatonta. Erilaiset laadun kohottamisen nimissä tehtävät kehittämishankkeet ilmentävät markkinataloustumista keskusjohtoisina ja nopeatempoisina. Onnistuessaan kehittämishanke luo perustan aidoille uudistuksille.

Panegírico del IV duque de Gandía, San Francisco de Borja

Oracion panegirica de San Pio V / que en su fiesta, transferida al dia veinte y dos de Mayo del presente año 1747 segundo de Pascua de Pentecostès,…

1747

Caplletres ornades, fris, vinyeta Sign.: *3, A-D4, E3 Notes a peu de pàg. Reclams

Dertonen Beatificationis, & Canonizationis san. mem. Pij Papae V

Vniuersis eiusdem Ordinis Religiosos Patribus, Fratribus, ac Sororibus Salutem, piumque iubilum in B. Pii Iubilaeo

Sign.: A2 Caplletra historiada