Search results for "590"
showing 10 items of 644 documents
Fitness Trade-Offs Determine the Role of the Molecular Chaperonin GroEL in Buffering Mutations
2015
Molecular chaperones fold many proteins and their mutated versions in a cell and can sometimes buffer the phenotypic effect of mutations that affect protein folding. Unanswered questions about this buffering include the nature of its mechanism, its influence on the genetic variation of a population, the fitness trade-offs constraining this mechanism, and its role in expediting evolution. Answering these questions is fundamental to understand the contribution of buffering to increase genetic variation and ecological diversification. Here, we performed experimental evolution, genome resequencing, and computational analyses to determine the trade-offs and evolutionary trajectories of Escherich…
Eggshell types and their evolutionary correlation with life-history strategies in squamates
2015
The eggshell is an important physiological structure for the embryo. It enables gas exchange, physical protection and is a calcium reserve. Most squamates (lizards, snakes, worm lizards) lay parchment-shelled eggs, whereas only some gekkotan species, a subgroup of lizards, have strongly calcified eggshells. In viviparous (live-bearing) squamates the eggshell is reduced or completely missing (hereafter “shell-less”). Recent studies showed that life-history strategies of gekkotan species differ between species with parchment- and rigid-shelled eggshells. Here we test if the three different eggshell types found in the squamates are also associated with different life-history strategies. We fir…
3D modul for syntetisk kalkulator
2006
Masteroppgave i informasjons- og kommunikasjonsteknologi 2006 - Høgskolen i Agder, Grimstad ActionScript er et språk som har blitt benyttet mer og mer de senere årene og er en del av verktøyet Flash. Dette er et design verktøy som har gode metoder for animasjoner i 2D og genererer små filer som gjør det svært egnet for internett. Denne rapporten ser på mulighetene for å generere, håndtere og visualisere tredimensjonale objekter i Flash ved bruk av ActionScript. Det blir diskutert roteringsmatriser opp mot Quaternion rotasjon og isometrisk projeksjon opp mot perspektivisk projeksjon i forhold til 3D objekter. Rapporten tar for seg ulike kurver og flater og hvilke begrensninger ActionScript h…
The ubiquitin-specific protease USP8 is critical for the development and homeostasis of T cells
2015
The modification of proteins by ubiquitin has a major role in cells of the immune system and is counteracted by various deubiquitinating enzymes (DUBs) with poorly defined functions. Here we identified the ubiquitin-specific protease USP8 as a regulatory component of the T cell antigen receptor (TCR) signalosome that interacted with the adaptor Gads and the regulatory molecule 14-3-3β. Caspase-dependent processing of USP8 occurred after stimulation of the TCR. T cell-specific deletion of USP8 in mice revealed that USP8 was essential for thymocyte maturation and upregulation of the gene encoding the cytokine receptor IL-7Rα mediated by the transcription factor Foxo1. Mice with T cell-specifi…