Search results for "70"

showing 10 items of 9933 documents

Little parallelism in genomic signatures of local adaptation in two sympatric, cryptic sister species.

2020

Species living in sympatry and sharing a similar niche often express parallel phenotypes as a response to similar selection pressures. The degree of parallelism within underlying genomic levels is often unexplored, but can give insight into the mechanisms of natural selection and adaptation. Here, we use multi-dimensional genomic associations to assess the basis of local and climate adaptation in two sympatric, cryptic Crematogaster levior ant species along a climate gradient. Additionally, we investigate the genomic basis of chemical communication in both species. Communication in insects is mainly mediated by cuticular hydrocarbons (CHCs), which also protect against water loss and, hence,…

0106 biological sciences0301 basic medicineSympatryClimateNicheGenome InsectAdaptation BiologicalBiology010603 evolutionary biology01 natural sciences03 medical and health sciencesddc:570AnimalsEcology Evolution Behavior and SystematicsLocal adaptationMutualism (biology)Phenotypic plasticityNatural selectionAntsBiological EvolutionHydrocarbonsAnimal CommunicationSympatry030104 developmental biologyEvolutionary biologySympatric speciationParallel evolutionJournal of evolutionary biologyREFERENCES
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Thioredoxin (Trxo1) interacts with proliferating cell nuclear antigen (PCNA) and its overexpression affects the growth of tobacco cell culture.

2017

Thioredoxins (Trxs), key components of cellular redox regulation, act by controlling the redox status of many target proteins, and have been shown to play an essential role in cell survival and growth. The presence of a Trx system in the nucleus has received little attention in plants, and the nuclear targets of plant Trxs have not been conclusively identified. Thus, very little is known about the function of Trxs in this cellular compartment. Previously, we studied the intracellular localization of PsTrxo1 and confirmed its presence in mitochondria and, interestingly, in the nucleus under standard growth conditions. In investigating the nuclear function of PsTrxo1 we identified proliferati…

0106 biological sciences0301 basic medicineTFs transcription factorsOverexpressionBiologíaBiFC bimolecular fluorescence complementationClinical BiochemistryCell Culture TechniquesTobacco BY-2 cells01 natural sciencesBiochemistryTBY-2 tobacco bright yellow-2DTT 14-dithiothreitolBimolecular fluorescence complementationThioredoxinsGene Expression Regulation PlantTrx thioredoxinlcsh:QH301-705.5GFP green fluorescent proteinlcsh:R5-920biologyProliferating cell nuclear antigen (PCNA)Cell cycleGlutathione3. Good healthCell biologyMitochondriaNTR NADPH thioredoxin reductaseProtein TransportDEM diethyl maleateRT-qPCR Reverse transcription quantitative polymerase chain reactionThioredoxinlcsh:Medicine (General)Oxidation-ReductionAMS 4-acetamido-4-maleimidylstilbene-22-disulfonic acidResearch PaperPCNA proliferating cell nuclear antigenOex overexpressingCell cycleNucleusThioredoxin o103 medical and health sciencesROS reactive oxygen speciesDownregulation and upregulationProliferating Cell Nuclear AntigenTobaccoDAPI 46-diamidine-2-phenylindolmCBM monochlorobimaneCellular compartmentCell NucleusCell growthOrganic ChemistryBotánicaPeasMolecular biologyYFP yellow fluorescent proteinProliferating cell nuclear antigenTBS Tris-buffered salineOD optical density030104 developmental biologylcsh:Biology (General)Cell cultureRNA reactive nitrogen speciesbiology.proteinPrx peroxiredoxinBSA bovine serum albumin010606 plant biology & botanyRedox biology
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Marine Cryptophytes Are Great Sources of EPA and DHA

2017

Microalgae have the ability to synthetize many compounds, some of which have been recognized as a source of functional ingredients for nutraceuticals with positive health effects. One well-known example is the long-chain polyunsaturated fatty acids (PUFAs), which are essential for human nutrition. Eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA) are the two most important long-chain omega-3 (-3) PUFAs involved in human physiology, and both industries are almost exclusively based on microalgae. In addition, algae produce phytosterols that reduce serum cholesterol. Here we determined the growth rates, biomass yields, PUFA and sterol content, and daily gain of eight strains of marine…

0106 biological sciences0301 basic medicineTROPICAL AUSTRALIAN MICROALGAELINOLENIC ACIDrasvahapotPharmaceutical Sciencesterols01 natural scienceschemistry.chemical_compoundFunctional FoodDrug DiscoveryFood scienceBiomasslcsh:QH301-705.5Pharmacology Toxicology and Pharmaceutics (miscellaneous)functional foodsPOLYUNSATURATED FATTY-ACIDSchemistry.chemical_classificationnutraceuticalsFRESH-WATERPRODUCTIVITYbiologymicroalgae; polyunsaturated fatty acids; omega-3; omega-6; sterols; functional foods; nutraceuticalsCHOLESTEROLmicroalgaeNANNOCHLOROPSISPhytosterolsfood and beveragesEicosapentaenoic acidEicosapentaenoic AcidDocosahexaenoic acidFatty Acids Unsaturatedlipids (amino acids peptides and proteins)omega-3CryptophytaPolyunsaturated fatty acidpolyunsaturated fatty acidsDocosahexaenoic Acidsomega-6CHEMICAL-COMPOSITIONterveysvaikutteiset elintarvikkeetfatty acidsGas Chromatography-Mass SpectrometryArticleMARICULTURE03 medical and health sciencesNutraceuticalAlgaeFatty Acids Omega-6Fatty Acids Omega-31172 Environmental sciencessterolit010604 marine biology & hydrobiologyomega fatty acidsta1183ta1182GROWTH-RATEmikrolevätbiology.organism_classificationSterolomegarasvahapot030104 developmental biologyHuman nutrition416 Food Sciencelcsh:Biology (General)chemistry13. Climate actionDietary SupplementsStearidonic acidMarine Drugs; Volume 16; Issue 1; Pages: 3
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Variable crab camouflage patterns defeat search image formation.

2021

Understanding what maintains the broad spectrum of variation in animal phenotypes and how this influences survival is a key question in biology. Frequency dependent selection – where predators temporarily focus on one morph at the expense of others by forming a “search image” – can help explain this phenomenon. However, past work has never tested real prey colour patterns, and rarely considered the role of different types of camouflage. Using a novel citizen science computer experiment that presented crab “prey” to humans against natural backgrounds in specific sequences, we were able to test a range of key hypotheses concerning the interactions between predator learning, camouflage and mor…

0106 biological sciences0301 basic medicineTime FactorsComputer scienceQH301-705.5BrachyuraBehavioural ecologyFrequency-dependent selectionMedicine (miscellaneous)ColorVariation (game tree)010603 evolutionary biology01 natural sciencesGeneral Biochemistry Genetics and Molecular BiologyArticlePredationtaskuravut03 medical and health sciencesDisruptive colorationCitizen scienceAnimalsHumansexperimental evolutionBiology (General)muuntelu (biologia)PredatorEcosystemsuojaväriMechanism (biology)Pigmentationbehavioural ecologyAdaptation Physiological030104 developmental biologyPhenotypeVideo GamesExperimental evolutionEvolutionary biologyCamouflagePattern Recognition PhysiologicalPredatory BehaviorfenotyyppiGeneral Agricultural and Biological SciencesColor PerceptionCommunications biology
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Technical Note on the quality of DNA sequencing for the molecular characterisation of genetically modified plants

2018

Abstract As part of the risk assessment (RA) requirements for genetically modified (GM) plants, according to Regulation (EU) No 503/2013 and the EFSA guidance on the RA of food and feed from GM plants (EFSA GMO Panel, 2011), applicants need to perform a molecular characterisation of the DNA sequences inserted in the GM plant genome. The European Commission has mandated EFSA to develop a technical note to the applicants on, and checking of, the quality of the methodology, analysis and reporting covering complete sequencing of the insert and flanking regions, insertion site analysis of the GM event, and generational stability and integrity. This Technical Note puts together requirements and r…

0106 biological sciences0301 basic medicineVeterinary (miscellaneous)[SDV]Life Sciences [q-bio]2405 Parasitologymolecularcharacterisationnext‐generation sequencingContext (language use)Plant ScienceComputational biologyGenetically modified cropsBiology01 natural sciencesMicrobiologyGenomeInsert (molecular biology)DNA sequencing03 medical and health sciencessymbols.namesake1110 Plant Sciencegenetically modified organismgenetic stabilityDNA sequencing1106 Food ScienceSanger sequencinggenetically modified organisms2404 Microbiologyrisk assessmentmolecular characterisation10079 Institute of Veterinary Pharmacology and ToxicologyGenetically modified organism3401 Veterinary (miscellaneous)Scientific Opinion030104 developmental biologyNGSsymbols570 Life sciences; biologyAnimal Science and ZoologyParasitologynext-generation sequencing1103 Animal Science and Zoology010606 plant biology & botanyFood ScienceVerification and validation
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Symptom severity, infection progression and plant responses in solanum plants caused by three pospiviroids vary with the inoculation procedure

2021

This article belongs to the Section Molecular Plant Sciences.

0106 biological sciences0301 basic medicineViroidvirusesPospiviroidaeCEVd01 natural sciencesRibosome18S ribosomal RNAAgro-infiltrationSolanum lycopersicumBiology (General)Spectroscopyfood and beveragesGeneral MedicinePSTVdTranscriptRibosomeViroidsComputer Science Applications02.- Poner fin al hambre conseguir la seguridad alimentaria y una mejor nutrición y promover la agricultura sostenibleChemistryRNA ViralPlasmidsQH301-705.5PospiviroidaeBiologyEggplantStressArticleCatalysisTomatoMicrobiologyInorganic Chemistry03 medical and health sciencesBIOQUIMICA Y BIOLOGIA MOLECULARPhysical and Theoretical ChemistryQD1-999Molecular BiologyPotato spindle tuber viroidPlant DiseasesInoculationOrganic ChemistryfungiRibosomal RNAbiology.organism_classification030104 developmental biologyTCDVdSolanumRibosomes010606 plant biology & botany
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Gene flow from an adaptively divergent source causes rescue through genetic and demographic factors in two wild populations of Trinidadian guppies

2016

Genetic rescue, an increase in population growth owing to the infusion of new alleles, can aid the persistence of small populations, but its use as a management tool is limited by a lack of empirical data geared towards predicting effects of gene flow on local adaptation and demography. Experimental translocations provide an ideal opportunity to monitor the demographic consequences of gene flow. In this study we take advantage of two experimental introductions of Trinidadian guppies to test the effects of gene flow on downstream native populations. We individually marked guppies from the native populations to monitor population dynamics for 3 months before and 26 months after gene flow. We …

0106 biological sciences0301 basic medicinecapture-mark-recaptureGenetic genealogyPoecilia reticulataPopulationdemographic rescueBiology010603 evolutionary biology01 natural sciencesGene flow03 medical and health sciencesgenetic rescueddc:570GeneticsAlleleeducationhybridizationEcology Evolution Behavior and SystematicsLocal adaptationGeneticseducation.field_of_studycapture‐mark‐recaptureEcotypePopulation sizeSmall population sizeOriginal Articlesfitness030104 developmental biologyväestönkasvuEvolutionary biologypopulation growthta1181Original ArticleGeneral Agricultural and Biological Sciencesgene flowEvolutionary Applications
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Unravelling the scientific debate on how to address wolf-dog hybridization in Europe

2019

Anthropogenic hybridization is widely perceived as a threat to the conservation of biodiversity. Nevertheless, to date, relevant policy and management interventions are unresolved and highly convoluted. While this is due to the inherent complexity of the issue, we hereby hypothesize that a lack of agreement concerning management goals and approaches, within the scientific community, may explain the lack of social awareness on this phenomenon, and the absence of effective pressure on decision-makers. By focusing on wolf x dog hybridization in Europe, we hereby (a) assess the state of the art of issues on wolf x dog hybridization within the scientific community, (b) assess the conceptual base…

0106 biological sciences0301 basic medicineconservation; delphi technique; genetic admixture; introgression; lethal removal; management; ethics; values in sciencelethal removallcsh:EvolutionPsychological interventionDelphi methodConservation; Delphi technique; Ethics; Genetic admixture; Introgression; Lethal removal; Management; Values in science;01 natural sciencesHYBRIDSATLANTIC SALMONlcsh:QH359-425ComputingMilieux_MISCELLANEOUSEcologyINTROGRESSIONconservationPublic relationsViewpointsConservation; Delphi technique; Ethics; Genetic admixture; Introgression; Lethal removal; Management; Values in scienceADMIXTUREDOMESTIC DOGSIdentification (biology)C180 EcologyDisciplinemanagementDETECTING HYBRIDIZATIONCONSERVATIONintrogressionC170 Population Biology010603 evolutionary biology03 medical and health sciencesWOLVESlcsh:QH540-549.5Political scienceValues in scienceMANAGEMENTSocial consciousnessEcology Evolution Behavior and SystematicsEthicsC300 Zoologybusiness.industryInterpretation (philosophy)C182 Evolutionconservation ; delphi technique ; genetic admixture ; introgression ; lethal removal ; management ; ethics ; values in science15. Life on landethics030104 developmental biologyConceptual frameworklcsh:Ecologydelphi techniquevalues in science[SDE.BE]Environmental Sciences/Biodiversity and EcologyCONSENSUSbusinessgenetic admixture
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The In Vitro Interaction of 12-Oxophytodienoic Acid and Related Conjugated Carbonyl Compounds with Thiol Antioxidants

2021

α,β-unsaturated carbonyls interfere with numerous plant physiological processes. One mechanism of action is their reactivity toward thiols of metabolites like cysteine and glutathione (GSH). This work aimed at better understanding these interactions. Both 12-oxophytodienoic acid (12-OPDA) and abscisic acid (ABA) conjugated with cysteine. It was found that the reactivity of α,β-unsaturated carbonyls with GSH followed the sequence trans-2-hexenal &lt

0106 biological sciences0301 basic medicinecysteine covalent modification570Isomerase activityArabidopsis thalianaArabidopsislcsh:QR1-50201 natural sciencesBiochemistryArticleAntioxidantslcsh:Microbiology03 medical and health scienceschemistry.chemical_compoundThioredoxinsPlant Growth RegulatorsmedicineCysteineSulfhydryl CompoundsMolecular BiologyCyclophilinchemistry.chemical_classificationChemistry<i>Arabidopsis thaliana</i>peroxiredoxinGlutathionethioredoxinphytohormones030104 developmental biologyMechanism of actionBiochemistryprotein–ligand interactioncyclophilinThiolFatty Acids Unsaturatedmedicine.symptomThioredoxinPeroxiredoxinthiol antioxidants010606 plant biology & botanyCysteineBiomolecules
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Sperm Quality Assessment in Honey Bee Drones

2020

The quality of honey bee drone semen is relevant in different contexts, ranging from colony productivity to pathology, toxicology and biodiversity preservation. Despite its importance, considerably less knowledge is available on this subject for the honey bee when compared to other domestic animal species. A proper assessment of sperm quality requires a multiple testing approach which discriminates between the different aspects of sperm integrity and functionality. Most studies on drone semen quality have only assessed a few parameters, such as sperm volume, sperm concentration and/or sperm plasma membrane integrity. Although more recent studies have focused on a broader variety of aspects …

0106 biological sciences0301 basic medicineendocrine systemsperm qualitymedia_common.quotation_subject<i>Apis mellifera</i>SemenReviewBiology01 natural sciencesGeneral Biochemistry Genetics and Molecular Biologyreproduction03 medical and health sciencesSemen qualitymaleQuality (business)Sperm qualitylcsh:QH301-705.5reproductive and urinary physiologymedia_commonSperm plasma membraneGeneral Immunology and Microbiologybusiness.industryurogenital systemfungisemenHoney beeSpermDroneBiotechnology010602 entomology030104 developmental biologylcsh:Biology (General)Apis melliferaGeneral Agricultural and Biological SciencesbusinessBiology
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