Search results for "81"
showing 10 items of 2468 documents
Nivolumab Enhances In Vitro Effector Functions of PD-1+ T-Lymphocytes and Leishmania-Infected Human Myeloid Cells in a Host Cell-Dependent Manner
2017
Functional impairment of T-cells and a concomitant augmented expression of programmed death-1 (PD-1) have been observed in visceral leishmaniasis patients, as well as in experimental models for visceral and cutaneous leishmaniasis. The PD-1/PD-1-ligand (PD-1/PD-L) interaction negatively regulates T-cell effector functions, which are required for parasite control during leishmaniasis. The aim of this study was to elucidate the impact of the PD-1/PD-L axis in a human primary in vitro infection model of Leishmania major (Lm). Blocking the PD-1/PD-L interaction with nivolumab increased T-cell proliferation and release of the proinflammatory cytokines TNFα and IFNγ during the cocultivation of Lm…
SIRM-SIAAIC consensus, an Italian document on management of patients at risk of hypersensitivity reactions to contrast media
2020
Abstract Hypersensitivity reactions (HRs) to contrast media (CM) can be distinguished in immune-mediated (including allergic reactions) and non-immune-mediated reactions, even if clinical manifestations could be similar. Such manifestations range from mild skin eruptions to severe anaphylaxis, making it important for radiologists to know how to identify and manage them. A panel of experts from the Società Italiana di Radiologia Medica e Interventistica (SIRM) and the Società Italiana di Allergologia, Asma e Immunologia Clinica (SIAAIC) provided a consensus document on the management of patients who must undergo radiological investigations with CM. Consensus topics included: the risk stratif…
Meeting the Challenges of High-Dimensional Single-Cell Data Analysis in Immunology
2019
Recent advances in cytometry have radically altered the fate of single-cell proteomics by allowing a more accurate understanding of complex biological systems. Mass cytometry (CyTOF) provides simultaneous single-cell measurements that are crucial to understand cellular heterogeneity and identify novel cellular subsets. High-dimensional CyTOF data were traditionally analyzed by gating on bivariate dot plots, which are not only laborious given the quadratic increase of complexity with dimension but are also biased through manual gating. This review aims to discuss the impact of new analysis techniques for in-depths insights into the dynamics of immune regulation obtained from static snapshot …
Macrophage: SHIP of Immunity
2014
Immunology. Why does it exist? Two words. Cure disease. People get diseases. “Test tubes” do not. People fund immunologists for solutions to their health problems. But, immunologists often study leukocytes in test tubes – the laboratory – away from diseases. Why? Because much can be learned from analyzing cellular biochemistry and behaviors in vitro that cannot be ascertained when leukocytes are in animals. At the same time, isolated leukocyte reactions often do not reflect how the immune system operates as a unit. So, it is critical to verify in vitro observations in vivo. Among leukocytes, macrophages are the central initiating and directing element in immune systems, and serve this role …
Bacterial and phytoplankton responses to nutrient amendments in a boreal lake differ according to season and to taxonomic resolution
2011
Nutrient limitation and resource competition in bacterial and phytoplankton communities may appear different when considering different levels of taxonomic resolution. Nutrient amendment experiments conducted in a boreal lake on three occasions during one open water season revealed complex responses in overall bacterioplankton and phytoplankton abundance and biovolume. In general, bacteria were dominant in spring, while phytoplankton was clearly the predominant group in autumn. Seasonal differences in the community composition of bacteria and phytoplankton were mainly related to changes in observed taxa, while the differences across nutrient treatments within an experiment were due to chang…
All-Possible-Couplings Approach to Measuring Probabilistic Context.
2013
From behavioral sciences to biology to quantum mechanics, one encounters situations where (i) a system outputs several random variables in response to several inputs, (ii) for each of these responses only some of the inputs may "directly" influence them, but (iii) other inputs provide a "context" for this response by influencing its probabilistic relations to other responses. These contextual influences are very different, say, in classical kinetic theory and in the entanglement paradigm of quantum mechanics, which are traditionally interpreted as representing different forms of physical determinism. One can mathematically construct systems with other types of contextuality, whether or not …
Retention forestry and biodiversity conservation: a parallel with agroforestry
2013
In forested landscapes two general management systems – retention forestry and agroforestry – have been proposed as potentially efficient components of landscape approaches to ease the conflict between biodiversity objectives and human needs. In two recent reviews, Gustafsson et al. (2012) and Lindenmayer et al. (2012) provide a global overview of current knowledge about the practice and ecological roles of retention forestry. A few years ago, Bhagwat et al. (2008) produced a similar review addressing the role of agroforestry in biodiversity conservation. Here we draw a parallel between research on the ecological effects of retention forestry and agroforestry. We argue that conservation sci…
Electrocardiographic Imaging Using a Spatio-Temporal Basis of Body Surface Potentials—Application to Atrial Ectopic Activity
2018
Electrocardiographic imaging (ECGI) strongly relies on a priori assumptions and additional information to overcome ill-posedness. The major challenge of obtaining good reconstructions consists in finding ways to add information that effectively restricts the solution space without violating properties of the sought solution. In this work, we attempt to address this problem by constructing a spatio-temporal basis of body surface potentials (BSP) from simulations of many focal excitations. Measured BSPs are projected onto this basis and reconstructions are expressed as linear combinations of corresponding transmembrane voltage (TMV) basis vectors. The novel method was applied to simulations o…
Relationship Between the Critical Power Test and a 20-min Functional Threshold Power Test in Cycling.
2021
To investigate the agreement between critical power (CP) and functional threshold power (FTP), 17 trained cyclists and triathletes (mean ± SD: age 31 ± 9 years, body mass 80 ± 10 kg, maximal aerobic power 350 ± 56 W, peak oxygen consumption 51 ± 10 mL⋅min–1⋅kg–1) performed a maximal incremental ramp test, a single-visit CP test and a 20-min time trial (TT) test in randomized order on three different days. CP was determined using a time-trial (TT) protocol of three durations (12, 7, and 3 min) interspersed by 30 min passive rest. FTP was calculated as 95% of 20-min mean power achieved during the TT. Differences between means were examined using magnitude-based inferences and a paired-samples…
External Load Variables Affect Recovery Markers up to 72 h After Semiprofessional Football Matches.
2019
Background: Player tracking devices are commonly used to monitor external load from training and matches in team sports. Yet, how the derived external load variables relate to fatigue and recovery post-training or post-match is scarcely researched. The objective was, therefore, to investigate how external load variables affect recovery markers up to 72 h post-match. Methods: Semiprofessional players from six teams wore tracking devices during three experimental football matches. External load variables including individual playing duration, total distance, PlayerLoad™, high-intensity running, and high-intensity events were derived from the tracking devices, and blood samples and performance…