Search results for "A2"
showing 10 items of 1101 documents
Evaluation of the MOD16A2 evapotranspiration product in an agricultural area of Argentina, the Pampas region
2021
The Pampas Region is a big plain of approximately 520,000 km2 in Argentina. It is essential to estimate evapotranspiration (ET) in this region since the primary productivity is directly linked to water availability. Information provided by satellite missions allows monitoring the spatial and temporal variability of ET. In the current study, we evaluated the version 006 of MOD16A2 product (MOD16A2.006) of Potential Evapotranspiration (ETp) and Actual Evapotranspiration (ETa) in Argentinian Pampas Region (APR). MOD16A2.006 product was compared with Crop Evapotranspiration (ETc), calculated with local measurements from the Oficina de Riesgo Agropecuario (ORA), and Crop Coefficient (Kc) data (f…
Identification of HLA-A*0201-Restricted T Cell Epitopes Derived from the Novel Overexpressed Tumor Antigen Calcium-Activated Chloride Channel 2
2002
Abstract Vaccination against tumor Ags may become a promising treatment modality especially in cancer types where other therapeutic approaches fail. However, diversity of tumors requires that a multitude of Ags become available. Differential expression in normal vs cancerous tissues, both at the mRNA and the protein level, may identify Ag candidates. We have previously compared transcripts from squamous cell lung cancer and normal lung tissue using differential display analysis, and found a transcript that was overexpressed in malignant cells and was identical with the calcium-activated chloride channel 2 (CLCA2) gene. We have now selected HLA-A2-restricted peptides from CLCA2, and have gen…
Quantitation of antigen-reactive T cells in peripheral blood by IFNgamma-ELISPOT assay and chromium-release assay: a four-centre comparative trial
2000
The ELISPOT assay is increasingly being used for the monitoring of the induction of antigen-reactive T cells in cancer vaccination trials. In order to evaluate the reliability of T cell frequency analysis with the ELISPOT assay, a comparative study was performed in four European laboratories. Six samples from healthy subjects were analyzed for the frequency of influenza-reactive CD8+ T cells in peripheral blood mononuclear cells (PBMC) by IFNgamma-ELISPOT assay. In addition, one laboratory determined cytotoxic T cell precursor (CTL) frequencies in these samples by limiting dilution chromium-release assay (LDA), and three laboratories performed a variant of the LDA, the multiple microculture…
Definition of the HLA-A2 restricted peptides recognized by human CD8+ effector T cells by flow-assisted sorting of the CD8+ CD45RA+ CD28– T cell subp…
2003
SUMMARY In response to antigenic stimulation, naive MHC-class I restricted and antigen-specific CD8+ CD45RA+ CD28+ T cells undergo clonal expansion, differentiate into CD8+ CD45RO+ memory T cells and convert to CD8+ CD45RA+ CD28− T cells displaying potent immune effector functions upon re-encounter with the nominal antigen. We show that the effector CD8+ CD45RA+ CD28– T cell subset is expanded in peripheral blood lymphocytes (PBL) from patients with human papilloma virus (HPV)+ cervical lesions as well as in PBL from patients with pulmonary tuberculosis. Flow-cytometric cell sorted CD8+ CD45RA+ CD28– and CD8+ CD45RA+ CD28– T cells were tested for recognition of HLA-A2 restricted peptides de…
Tumor associated antigens in human renal cell carcinoma: MHC restricted recognition by cytotoxic T lymphocytes.
1996
Based on previous studies in human melanoma leading to the molecular cloning of genes encoding peptide antigens recognized by MHC-restricted cytotoxic T lymphocytes (CTL) we extended our efforts to renal cancer systems established in tissue culture. In two patients we obtained stable CD8+ CTL clones with high cytolytic activity for the corresponding autologous tumor cell line in vitro. Neither autologous EBV-transformed B lymphocytes or PHA-activated PBL nor natural killer targets K562 were lysed by these CTL clones. MZ1257-RCC CTL5-30 lysed autologous tumor cells as well as normal kidney cell cultures in an HLA-A2 restricted fashion. Further specificity analysis showed cross reactivity wit…
Women in technology-oriented fields
2015
This study focuses on investigating the main elements that have an effect on women’s decisions to enter a career in technology-oriented fields, and more specifically, to discover whether studying crafts, especially technical work, during basic education affects their decisions in this context. The study was carried out using a semi-structured questionnaire, and the data consist of the responses from 12 female technology education teachers and 12 female engineering students. A qualitative theory oriented content analysis was carried out through identifying, coding, analysing and reporting the patterns within the data. The findings revealed that the most influential career anchor identified b…
Vacancy Defects in Ga2O3: First-Principles Calculations of Electronic Structure
2021
This research was funded by the Science Committee of the Ministry of Education and Science of the Republic of Kazakhstan (Grant No. AP08856540) as well as by the Latvian research council via the Latvian National Research Program under the topic ?High-Energy Physics and Accelerator Technologies?, Agreement No: VPP-IZM-CERN-2020/1-0002 for A.I. Popov. In addition, J. Purans is grateful to the ERAF project 1.1.1.1/20/A/057 while A. Platonenko was supported by Latvian Research Council No. LZP-2018/1-0214. The authors thank A. Lushchik and M. Lushchik for many useful discussions. The research was (partly) performed in the Institute of Solid State Physics, University of Latvia ISSP UL. ISSP UL as…
Plasmonic nanostructures through DNA-assisted lithography
2018
DALI combines DNA origami with conventional top-down fabrication for creating designer high-resolution plasmonic nanostructures.
Role of three isoforms of phospholipase A2in capacitative calcium influx in human T-cells
2002
The present study was conducted on human Jurkat T-cell lines in order to elucidate the role of phospholipase A2 in capacitative calcium entry. We have employed thapsigargin (TG) that induces increases in [Ca2+]i by emptying the calcium pool of endoplasmic reticulum, followed by capacitative calcium entry. We designed a Ca2+ free/Ca2+ reintroduction (CFCR) protocol for the experiments, conducted in Ca2+-free medium. By employing CFCR protocol, we observed that addition of exogenous arachidonic acid (AA) stimulated TG-induced capacitative calcium influx. The liberation of endogenous AA and its autocrine action seems to be implicated during TG-induced capacitative calcium influx: TG potentiate…
Optimal Resource Discovery Paths of Gnutella2
2008
This paper shows that the performance of peer-to-peer resource discovery algorithms is upper bounded by a k-Steiner minimum tree and proposes an algorithm locating near-optimal query paths for the peer-to-peer resource discovery problem. Global knowledge of the topology and the resources from the peer-to-peer network are required as an input to the algorithm. The algorithm provides an objective measure for defining how good local search algorithms are. The performance is evaluated in simulated peer-to-peer scenarios and in the measured Gnutella2 P2P network topology with four local search algorithms: breadth-first search, self-avoiding random walker, highest degree search and Dynamic Query …