Search results for "ACID"
showing 10 items of 13107 documents
Activation of astroglial phospholipase D activity by phorbol ester involves ARF and Rho proteins.
2000
Primary cultures of rat cortical astrocytes express phospholipase D (PLD) isoforms 1 and 2 as determined by RT-PCR and Western blot. Basal PLD activity was strongly (10-fold) increased by 4beta-phorbol-12beta,13alpha-dibutyrate (PDB) (EC(50): 56 nM), an effect which was inhibited by Ro 31-8220 (0.1-1 microM), an inhibitor of protein kinase C (PKC), and by brefeldin A (10-100 microg/ml), an inhibitor of ADP-ribosylating factor (ARF) activation. Pretreatment of the cultures with Clostridium difficile toxin B-10463 (0.1-1 ng/ml), which inactivates small G proteins of the Rho family, led to a breakdown of the astroglial cytoskeleton; concomitantly, PLD activation by PDB was reduced by up to 50%…
Metabotropic glutamate receptors activate phospholipase D in astrocytes through a protein kinase C-dependent and Rho-independent pathway.
2003
Metabotropic glutamate receptors (mGluRs) are G protein-coupled receptors that mediate phospholipase D (PLD) activation in brain, but the mechanism underlying this response remains unclear. Here we used primary cultures of astrocytes as a cell model to explore the mechanism that links mGluRs to PLD. Glutamate activated both phospholipase C (PLC) and PLD with equal potency and this effect was mimicked by L-cysteinesulfinic acid, a putative neurotransmitter previously shown to activate mGluRs coupled to PLD, but not PLC, in adult brain. PLD activation by glutamate was dependent on Ca(2+) mobilization and fully blocked by both protein kinase C (PKC) inhibitors and PKC down-regulation, suggesti…
Inhibition of Rho modulates cytokine-induced prostaglandin E2 formation in renal mesangial cells.
2003
Stimulation of rat mesangial cells for 24 h with interleukin-1β(IL-1β) plus forskolin (Fk) leads to a marked increase in prostaglandin E 2 (PGE 2 ) synthesis. This effect is further enhanced by the small G-protein Rho inhibitor toxin A. A similar increase in PGE 2 formation is obtained with Y27632, a Rho-dependent kinase inhibitor, and with lovastatin, a hydroxymethylglutaryl-coenzyme A inhibitor which depletes cells from geranylgeranyl moieties and thus blocks Rho activation. In parallel to the increased PGE 2 synthesis, a potentiation of IL-1β-induced secretory group IIA phospholipases A 2 (sPLA 2 -IIA) protein expression also occurs by Rho inhibition. However, only toxin A triggers an in…
Trattamento infiltrativo ecoguidato d'anca versus infiltrazioni associate a terapia riabilitativa: studio randomizzato controllato
2013
La coxartrosi è una malattia, caratterizzata da deterioramento della cartilagine e delle strutture articolari, che si manifesta clinicamente con dolore ingravescente e conseguente riduzione della funzionalità. Presso l'U.O.C. di Riabilitazione dell'A.O.U.P. Paolo Giaccone, tra febbraio 2008 e gennaio 2013 sono stati reclutati 224 soggetti sottoposti ad un ciclo infiltrativo che prevedeva 3 somministrazioni intrarticolari di acido ialuronico, eseguite sotto guida ecografica, a distanza di 45 giorni l'una dall'altra; i pazienti sono stati suddivisi, in modo random, in 2 gruppi (gruppo A e gruppo B). Il gruppo A è stato sottoposto solamente a trattamento infiltrativo; al gruppo B è stato assoc…
The Effect of Mechanocatalytic Pretreatment on the Structure and Depolymerization of Willow
2020
In this study, the effect of a mechanocatalytic pretreatment on the structure of willow and sugar release from pretreated willow was explored. In the mechanocatalytic approach, the pretreatment consists of solvent-free impregnation with sulfuric acid and a mechanical treatment with ball milling. Willow sawdust and pretreated samples were analyzed with field emission scanning electron microscope and X-ray diffraction. The products in the sugar solution were determined as the total reducing sugars with the 3,5-dinitrosalicylic acid method and monosaccharides with capillary electrophoresis. According to the results, milling increased the sugar production, depending on the sulfuric acid load. T…
Efecto del juego vs. el role-playing en la implicación de los participantes del Programa saludiversex
2021
El programa de educación afectivo sexual SALUDIVERSEX para personas con diversidad funcional intelectual (DFI) sienta sus bases sobre la importancia de la implicación activa de los participantes en las sesiones dirigidas a favorecer el aprendizaje de conocimientos, habilidades y actitudes saludables. Por ello, en las sesiones dedicadas a transmitir conocimientos sobre sexo/sexualidad, higiene sexual, prácticas sexuales y sexo seguro, así como, en las que se trabajan actitudes hacia la diversidad sexual y las relaciones de pareja se prioriza más el juego. Por otra parte, en las sesiones dedicadas a trabajar habilidades interpersonales se emplea fundamentalmente el role playing. El objetivo d…
NMR-based quantification of rosmarinic and carnosic acids, GC-MS profile and bioactivity relevant to neurodegenerative disorders of Rosmarinus offici…
2013
Abstract A comparative study of phytochemicals content and biological properties of eight Rosmarinus officinalis (rosemary) populations (RO1–RO8) collected in different areas of Tunisia was carried out. Two of the main rosemary constituents, rosmarinic and carnosic acids, were quantified by an NMR technique. Carnosic acid content was higher than that of rosmarinic acid. The non-polar constituents were examined by GC and GC–MS. Total phenols and flavonoids content were also determined in order to discuss the possible correlation between these phytochemicals and bioactivity. Antioxidant activity was investigated through different in vitro assays. Sample RO3 from a sub-humid area showed the hi…
Development of high-performance thin layer chromatography method for identification of phenolic compounds and quantification of rosmarinic acid conte…
2020
ABSTRACT Aim: Representatives of Nepetoideae Burnett subfamily are promising species of the Lamiaceae Martinov family because of accumulating such valuable groups of secondary metabolites as terpenoids and polyphenols. A high-performance thin layer chromatography (HPTLC) fingerprint method for the qualitative determination of phenolic compounds and for the quantification of rosmarinic acid (RA) content in methanol extracts of five species of this subfamily was developed for the first time. Materials and Methods: Dried aerial parts of Dracocephalum moldavica L., Ocimum americanum L., Lophanthus anisatus (Nutt.) Benth., Monarda fistulosa L., and Satureja hortensis L. collected in flowering pe…
SMART NANOSPONGE-BASED SYSTEMS FOR ADVANCED APPLICATIONS
2023
Ruolo del rumore ambientale in sistemi complessi di natura biologica
2010
La Fisica dei Sistemi Complessi ha recentemente assunto un ruolo sempre più importante nella descrizione dei sistemi biologici a causa delle interazioni, sia deterministiche sia rumorose o “randomiche”, di tali sistemi con l’ambiente. La presenza del rumore diviene particolarmente rilevante nella trattazione dei sistemi biologici in ambito medico. In questo lavoro vengono presentati due diversi sistemi: i) un modello di dinamica di popolazioni che descrive lo sviluppo delle cellule tumorali responsabili della Leucemia Mieloide Cronica (CML); ii) un modello stocastico che riproduce la crescita di batteri in alimenti di origine animale. Nel primo sistema viene utilizzato un approccio Monte Ca…