Search results for "ADULT"

showing 10 items of 17453 documents

Reducing diagnostic turnaround times of exome sequencing for families requiring timely diagnoses

2017

IF 2.137; International audience; BACKGROUND AND OBJECTIVE:Whole-exome sequencing (WES) has now entered medical practice with powerful applications in the diagnosis of rare Mendelian disorders. Although the usefulness and cost-effectiveness of WES have been widely demonstrated, it is essential to reduce the diagnostic turnaround time to make WES a first-line procedure. Since 2011, the automation of laboratory procedures and advances in sequencing chemistry have made it possible to carry out diagnostic whole genome sequencing from the blood sample to molecular diagnosis of suspected genetic disorders within 50 h. Taking advantage of these advances, the main objective of the study was to impr…

0301 basic medicineAdultMaleExome sequencingmedicine.medical_specialtyTime FactorsAdolescentGenetic counselingBioinformaticsTurnaround timeSensitivity and SpecificityUndiagnosed genetic conditions03 medical and health sciencesGeneticsmedicineHumansExomeGenetic TestingMedical diagnosisIntensive care medicineChildExomeGenetics (clinical)Exome sequencingGenetic testingWhole genome sequencing[SDV.GEN]Life Sciences [q-bio]/Geneticsmedicine.diagnostic_testbusiness.industryInfant NewbornInfantGeneral MedicineSequence Analysis DNADiagnostic turnaround time3. Good healthClinical trial030104 developmental biologyEarly DiagnosisChild PreschoolFemalebusiness[ SDV.GEN ] Life Sciences [q-bio]/Genetics
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No long-term effect of a 2-days intervention on how to prepare homemade food, on toddlers’ skepticism for new food and intake of fruits and vegetable…

2017

Objective Optimal nutrition from early age reduces the risk of developing non-communicable diseases later in life. The aim of this study was to examine the long-term effect on toddlers’ fruit and vegetable intake and sweet beverages, and skepticism for new food, of a 2-days’ intervention on how to prepare homemade food for toddlers. Results The effect of the cooking intervention was evaluated by a randomized, controlled trial where 110 parents of 4–6 months old infants were included. Child diet and food skepticism were measured at 6, 15 and 24 months of age. There were no differences between the control and intervention group in the consumption of fruits and vegetables and intake of water o…

0301 basic medicineAdultMaleFood skepticismDietary Sugarsmedia_common.quotation_subjectlcsh:MedicineIntervention groupGeneral Biochemistry Genetics and Molecular Biologylaw.inventionBeverages03 medical and health sciencesEatingFood PreferencesRandomized controlled triallawFood intakeIntervention (counseling)Environmental healthVegetablesMedicineHumansTerm effectFood scienceCookinglcsh:Science (General)Trial registrationlcsh:QH301-705.5Skepticismmedia_commonToddlers030109 nutrition & dieteticsbusiness.industrylcsh:Rdigestive oral and skin physiologyInfantGeneral MedicineDietResearch Notelcsh:Biology (General)Optimal nutritionFruits and vegetablesFruitFemaleInfant Foodbusinesslcsh:Q1-390Cooking courseBMC Research Notes
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In vivo muscle morphology comparison in post-stroke survivors using ultrasonography and diffusion tensor imaging.

2019

AbstractSkeletal muscle architecture significantly influences the performance capacity of a muscle. A DTI-based method has been recently considered as a new reference standard to validate measurement of muscle structure in vivo. This study sought to quantify muscle architecture parameters such as fascicle length (FL), pennation angle (PA) and muscle thickness (tm) in post-stroke patients using diffusion tensor imaging (DTI) and to quantitatively compare the differences with 2D ultrasonography (US) and DTI. Muscle fascicles were reconstructed to examine the anatomy of the medial gastrocnemius, posterior soleus and tibialis anterior in seven stroke survivors using US- and DTI-based techniques…

0301 basic medicineAdultMaleFysiologiPhysiologylcsh:MedicineSkeletal musclelihaksettuki- ja liikuntaelimetArticle03 medical and health sciencesMuscle morphology0302 clinical medicinemorfologiaIn vivomedicineHumansin vivo -menetelmädiffuusiotensorikuvausSurvivorsskeletal musclelcsh:ScienceMuscle SkeletalAgedUltrasonographyMultidisciplinarybusiness.industrylcsh:RSkeletal muscleMiddle AgedIndividual leveldiffusion tensor imagingStroke030104 developmental biologymedicine.anatomical_structureDiffusion Tensor ImagingPost strokelcsh:QFemaleUltrasonographyMuscle architecturebusinessBiomedical engineering030217 neurology & neurosurgeryBiomedical engineeringDiffusion MRIScientific reports
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Dynamics of Torque Teno virus plasma DNAemia in allogeneic stem cell transplant recipients

2017

Abstract Background Torque Teno virus (TTV) plasma DNA load directly correlate with the level of immunosuppresion in different clinical settings. It is uncertain whether this may be the case in allogeneic hematopoietic stem cell transplant recipients (allo-HSCT). Objectives We characterized the dynamics of TTV DNAemia in patients undergoing T-cell replete allo-SCT. Study design Retrospective single-center observational study including 72 allo-HSCT patients. Plasma TTV DNA loads were quantified before initiating the conditioning regimen and at different time-points after transplant by real-time PCR. White blood cells (WBC) and absolute lymphocyte counts (ALC) were measured by flow cytometry.…

0301 basic medicineAdultMaleHomologousTorque teno virusAdolescentLymphocyteAllogeneic hematopoietic stem cell transplantation (Allo-HSCT); Immune reconstitution; Torque Teno virus (TTV); TTV DNAemia; Adolescent; Adult; Aged; DNA Virus Infections; DNA Viral; Female; Humans; Immunocompromised Host; Leukocyte Count; Male; Middle Aged; Retrospective Studies; Torque teno virus; Transplantation Homologous; Viral Load; Viremia; Young Adult; Hematopoietic Stem Cell Transplantation; Virology; Infectious Diseases030106 microbiologyTTV DNAemiaFlow cytometry03 medical and health sciencesImmunocompromised HostLeukocyte CountYoung AdultVirologyMedicineTransplantation HomologousHumansIn patientViralViremiaProspective cohort studyAgedRetrospective StudiesTorque teno virusTransplantationmedicine.diagnostic_testbusiness.industryHematopoietic Stem Cell TransplantationDNAImmune reconstitutionMiddle AgedViral LoadDNA Virus Infectionssurgical procedures operative030104 developmental biologymedicine.anatomical_structureInfectious DiseasesAllogeneic hematopoietic stem cell transplantation (Allo-HSCT); Immune reconstitution; Torque Teno virus (TTV); TTV DNAemia; Adolescent; Adult; Aged; DNA Virus Infections; DNA; Viral; Female; Humans; Immunocompromised Host; Leukocyte Count; Male; Middle Aged; Retrospective Studies; Torque teno virus; Transplantation; Homologous; Viral Load; Viremia; Young Adult; Hematopoietic Stem Cell Transplantation; Virology; Infectious DiseasesImmunologyDNA ViralAllogeneic hematopoietic stem cell transplantation (Allo-HSCT)FemaleAllogeneic hematopoietic stem cell transplantStem cellImmunocompetencebusinessTorque Teno virus (TTV)
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Thermosensory mapping of skin wetness sensitivity across the body of young males and females at rest and following maximal incremental running

2019

Key points: Humans lack skin receptors for wetness (i.e. hygroreceptors), yet we present a remarkable wetness sensitivity. Afferent inputs from skin cold-sensitive thermoreceptors are key for sensing wetness; yet, it is unknown whether males and females differ in their wetness sensitivity across their body and whether high intensity exercise modulates this sensitivity. We mapped sensitivity to cold, neutral and warm wetness across five body regions and show that females are more sensitive to skin wetness than males, and that this difference is greater for cold than warm wetness sensitivity. We also show that a single bout of maximal exercise reduced the sensitivity to skin wetness (i.e. hyg…

0301 basic medicineAdultMaleHot TemperaturePhysiologyRestSensationPhysiologySensory systemBiologyWetnessRunning03 medical and health sciences0302 clinical medicineCutaneous receptorPhysical StimulationSkin Physiological PhenomenaSensationHumansThermosensingSensitivity (control systems)Skin wetnessExerciseSkinBody surface areaSex CharacteristicsFootThermoreceptorsCold Temperature030104 developmental biologyTouch PerceptionTouchThermoreceptorBody regionFemaleSexSkin Temperature030217 neurology & neurosurgeryBody Temperature Regulation
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Secular trends in Dietary Inflammatory Index among adults in the United States, 1999-2014.

2018

Objective: The objective of this study was to evaluate secular trends in Dietary Inflammatory Index (DII) scores in the United States between 1999 and 2014. Methods: Data from adults over 19 years from the 1999 to 2014 National Health and Nutrition Examination Survey (N = 39,191) were used. DII scores, at each 2-year cycle, were evaluated from a 24-h recall, including 26 food parameters for DII calculation. Analyses were conducted in 2018. Results: For the entire sample, there was a quadratic trend (Ptrend < 0.001), with the DII scores peaking in 2003–2004, and then decreasing during the cycles from 2005 to 2014. Similar quadratic trends (Ptrend < 0.001) were observed by age, gender, …

0301 basic medicineAdultMaleIndex (economics)National Health and Nutrition Examination SurveyPopulationEthnic groupCANCER-RISKMedicine (miscellaneous)Black People030209 endocrinology & metabolismWhite People03 medical and health sciences0302 clinical medicineMexican AmericansEthnicityMedicineHumansSOCIAL DESIRABILITYeducationInflammationeducation.field_of_study030109 nutrition & dieteticsNutrition and Dieteticsbusiness.industryMiddle AgedNutrition SurveysQuadratic trendC-REACTIVE PROTEINeye diseasesUnited StatesSecular variationDietChronic diseaseYounger adultsHEALTHY EATING INDEXChronic DiseaseEducational StatusFemalesense organsbusinessDemographyEuropean journal of clinical nutrition
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M1 Macrophages Activate Notch Signalling in Epithelial Cells: Relevance in Crohn's Disease

2016

Background: The Notch signalling pathway plays an essential role in mucosal regeneration, which constitutes a key goal of Crohn's disease (CD) treatment. Macrophages coordinate tissue repair and several phenotypes have been reported which differ in the expression of surface proteins, cytokines and hypoxia-inducible factors (HIFs). We analysed the role of HIFs in the expression of Notch ligands in macrophages and the relevance of this pathway in mucosal regeneration. Methods: Human monocytes and U937-derived macrophages were polarized towards the M1 and M2 phenotypes and the expression levels of HIF-1α, HIF-2α, Jagged 1 (Jag1) and delta-like 4 (Dll4) were evaluated. The effects of macrophage…

0301 basic medicineAdultMaleJAG1FarmacologiaAdolescentEnterocyteColonNotch signaling pathwayBiologymucosal healing03 medical and health sciencesYoung AdultIntestinal mucosaCrohn DiseasemedicineMacrophageHumansHES1Intestinal MucosaRecte MalaltiesReceptors NotchMacrophagesGastroenterologyEpithelial CellsGeneral MedicineMiddle AgedHypoxia-Inducible Factor 1 alpha SubunitCoculture TechniquesCell biologyCrohn's disease030104 developmental biologymedicine.anatomical_structureAparell digestiu MalaltiesCase-Control StudiesImmunologyLeukocytes MononuclearCytokinesNotch signallingEnterocyte differentiationFemaleOriginal ArticleSignal transductionCaco-2 CellsHT29 CellsBiomarkersSignal Transduction
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Microbial Activation of Wooden Vats Used for Traditional Cheese Production and Evolution of Neoformed Biofilms.

2016

ABSTRACT Three Lactococcus lactis subsp. cremoris strains were used to develop ad hoc biofilms on the surfaces of virgin wooden vats used for cheese production. Two vats (TZ) were tested under controlled conditions (pilot plant), and two vats (TA) were tested under uncontrolled conditions (industrial plant). In each plant, one vat (TA1 and TZ1) was used for the control, traditional production of PDO Vastedda della Valle del Belìce (Vastedda) cheese, and one (TA2 and TZ2) was used for experimental production performed after lactococcal biofilm activation and the daily addition of a natural whey starter culture (NWSC). Microbiological and scanning electron microscopy analyses showed differenc…

0301 basic medicineAdultMaleLactococcus030106 microbiologyBiologyBacterial Physiological PhenomenaLactic acid bacteria; traditional cheeseApplied Microbiology and Biotechnology03 medical and health sciencesSettore AGR/17 - Zootecnica Generale E Miglioramento Genetico0404 agricultural biotechnologyStartertraditional cheeseCheeseLactic acid bacteriaFood microbiologyAnimalsHumansFood scienceEcologyBacteriaLactococcus lactisBiofilm04 agricultural and veterinary sciencesBiodiversityMiddle Agedbiology.organism_classification040401 food scienceWoodLactococcus lactisLactic acid bacteriumMilkBiofilmsTasteFood MicrobiologyFemaleBacteriaFood ScienceBiotechnologyMesophileSettore AGR/16 - Microbiologia AgrariaApplied and environmental microbiology
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Examining the "Veggie" personality: Results from a representative German sample.

2017

Abstract An increasing proportion of people choose to follow a vegetarian diet. To date, however, little is known about if and how individual differences in personality relate to following a vegetarian diet. In the two studies presented here, we aimed to (1) estimate the prevalence of self-defined vegetarians in two waves of a German representative sample (N = 4496 and 5125, respectively), (2) analyze the effect of socio-demographic variables on dietary behavior, and (3) examine individual differences between vegetarians and meat eaters in personality traits, political attitudes, and health-related variables. In Study 1, a strict definition of vegetarians was used, while in Study 2 the defi…

0301 basic medicineAdultMaleMeatmedia_common.quotation_subjectIndividualitySample (statistics)ConservatismGerman03 medical and health sciences0302 clinical medicineGermanyOpenness to experiencePersonalityHumans030212 general & internal medicineBig Five personality traitsGeneral Psychologymedia_common030109 nutrition & dieteticsNutrition and DieteticsDiet VegetarianPoliticsConscientiousnessFeeding BehaviorStepwise regressionMiddle Agedlanguage.human_languageDietAttitudelanguageFemalePsychologySocial psychologyDemographyPersonalityVegetariansAppetite
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Effect of laser treatment on postural control parameters in patients with chronic nonspecific low back pain: a randomized placebo-controlled trial

2019

The management of nonspecific lumbar pain (NSLP) using laser irradiation remains controversial. A systematic review of recently published studies indicates that the effects of laser therapy are commonly assessed using only imperfect methods in terms of measurement error. The main objective of this study was to assess static postural stability using an objective tool in patients with chronic NSLP after laser irradiation at different doses and wavelengths. In total, 68 patients were included in the laser sessions and were randomly assigned into four groups: high-intensity laser therapy at 1064 nm and 60 J/cm2 for 10 min (HILT), sham (HILT placebo), low-level laser therapy at 785 nm and 8 J/cm…

0301 basic medicineAdultMaleMedicine (General)QH301-705.5PhysiologyImmunologyBiophysicsPlacebo-controlled studyOcean EngineeringPlaceboBiochemistrylaw.invention03 medical and health sciencesR5-9200302 clinical medicineLumbarRandomized controlled trialLaser therapylawPostural BalanceMedicineHumansLow back painBiology (General)General Pharmacology Toxicology and PharmaceuticsLead (electronics)Pain Measurementbusiness.industryPhysical therapy modalitiesGeneral NeuroscienceCell BiologyGeneral MedicineMiddle AgedLow back pain030104 developmental biologyTreatment Outcome030220 oncology & carcinogenesisAnesthesiaPostural balanceChronic DiseaseFemaleAnalysis of variancemedicine.symptombusinessResearch ArticleFollow-Up StudiesBrazilian Journal of Medical and Biological Research
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