Search results for "AFLP"

Assessment of genetic variation in SicilianHelichrysum(Asteraceae) and implication to germplasm conservation

2008

Some taxa of Sicilian Helichrysum related to sect. Stoeachadina have an uncertain systematic position; moreover, they are rare and endangered. In order to preserve these taxa in a genebank, a strategy to keep their genetic variation as high as possible is required. The high informative content of AFLP markers was used to assess genetic variations within and between populations and their relationships. The results show that all populations are genetically similar, even though there are two distinct groups: the first is the population referred to H. pendulum, and the second is represented by other populations belonging to several different specific and intraspecific taxa. The H. pendulum popu…

Further investigations on populations of the deep-water blue and red shrimp Aristeus antennatus (Risso, 1816) (Decapoda, Dendrobranchiata), as inferr…

2012

The aim of this study was to integrate existing mitochondrial DNA data relating to the deep-sea blue and red shrimp Aristeus antennatus (Risso, 1816) with data obtained by Amplified Fragment Length Polymorphism (AFLP). A total of 145 AFLP polymorphic loci were scored in 236 specimens collected from one Atlantic and seven Mediterranean sample sites. AMOVA results revealed that the overall genetic variation among-populations was lower (11.81%) than within-populations (88.19%). The genetic variation between the Atlantic and Mediterranean samples was found to be not significant ( Φ CT = − 0 . 007 ; N.S.), indicating that the transition area between the Atlantic Ocean and the Mediterranean Sea d…

A polyphasic approach to the identification of ochratoxin A-producing black Aspergillus isolates from vineyards in Sicily.

2008

Aspergillus strains belonging to section Nigri isolated during a two year survey in eight Sicilian vineyards located on the slopes of Mount Etna (Sicily, Italy) were analysed analyzed in order to characterize species responsible for ochratoxin A (OTA) contamination of grapes. The polyphasic approach permitted analysis of biodiversity of Aspergillus isolates in relation to their morphology, ochratoxigenicity and genetic variability. We assessed OTA production by A. carbonarius, A. niger, A. tubingensis and A. japonicus using an enzyme-linked immunosorbent assay. A. carbonarius isolates were the strongest OTA producers. A subset of 66 representative strains was selected for further DNA-based …

Molecular diagnostic tools for the detection and characterization of Phoma tracheiphila

2011

In the recent years PCR-based techniques for the identification and detection of Phoma tracheiphila, the causal agent of citrus mal secco disease, have been evaluated aiming to provide tools for biological and epidemiological studies. A wide collection of P. tracheiphila strains was used to evaluate and validate diagnostic protocols and a fAFLP method for fungal characterization. Conventional and real-time PCR protocols were successfully tested for the specific identification of P. tracheiphila and its detection in planta. A further improvement of the real-time PCR protocol and the DNA extraction methods allowed the quantification of the fungus both from naturally infected and artificially …

Evaluation of a modified single-enzyme amplified fragment length polymorphism (SE-AFLP) technique for subtyping Salmonella enterica serotype Enteriti…

2006

Salmonella enterica subsp. enterica serotype Enteritidis is not readily subtyped beyond the level of phage type (PT). Pulsed field gel electrophoresis (PFGE) is generally acknowledged to be the most discriminating typing method for Salmonella, but only a restricted variety of PFGE types has been described for S. enterica serotype Enteritidis. In the present study, a modification of the SE-AFLP typing method was used to investigate both outbreak and apparently sporadic isolates of S. enterica serotype Enteritidis belonging to different PTs and/or PFGE types. The method proved to be as discriminatory as PFGE when combined with phage typing, and provided subtyping data consistent with epidemio…

Use of pulsed field gel electrophoresis (PFGE) and single-enzyme amplified fragment lenght polymorphism (SE-AFLP) to subtype isolates of Salmonella e…

2005

Population genetics on Ambrosia genus: past, present and future

2014

National audience; A new Task Force within the COST-SMARTER has recently been created. This group is composed of population genetics experts from five European countries in charge of focusing on “Genetics on Ambrosia” (Austria, France, Germany, Hungary, Sweden). Their mission was to review molecular methods used in published genetics studies on common ragweed and to discuss about future projects. Two different types of molecular markers are generally used in the literature to investigate the genetic structure within and between introduced and/or native populations of common ragweed: microsatellite markers and amplified fragment length polymorphism (AFLP). These methods permit the researcher…

Characterization of Alternaria alternata Isolates from Tangerine Hybrids Affected by Brown Spot in Italy

2011

Alternaria brown spot, caused by Alternaria alternata, is the most important disease of tangerines and their hybrids worldwide, including Italy. The disease represents, for some susceptible citrus cultivar, a limiting factor in profitable cultivation. The results of the characterization of A. alternata isolates obtained from lesions on leaves and fruits of Fortune, Nova and Winola hybrids and Valencia sweet orange, collected in two groves located in Calabria and Sicily, is reported. An endopolygalacturonase gene (endoPG) was sequenced from five representative strains and a phylogenetic tree based on endoPG sequence data of Italian isolates and those of citrus associated Alternaria species d…

Data from: Successive invasion-mediated interspecific hybridizations and population structure in the endangered cichlid Oreochromis mossambicus

2013

Hybridization between invasive and native species accounts among the major and pernicious threats to biodiversity. The Mozambique tilapia Oreochromis mossambicus, a widely used freshwater aquaculture species, is especially imperiled by this phenomenon since it is recognized by the IUCN as an endangered taxon due to genetic admixture with O. niloticus an invasive congeneric species. The Lower Limpopo and the intermittent Changane River (Mozambique) drain large wetlands of potentially great importance for conservation of O. mossambicus, but their populations have remained unstudied until today. Therefore we aimed (1) to estimate the autochthonous diversity and population structure among genet…