Search results for "ALPHA-S"
showing 10 items of 45 documents
A novel bio-orthogonal cross-linker for improved protein/protein interaction analysis
2015
International audience; The variety of protein cross-linkers developed in recent years illustrates the current requirement for efficient reagents optimized for mass spectrometry (MS) analysis. To date, the most widely used strategy relies on commercial cross-linkers that bear an isotopically labeled tag and N-hydroxysuccinimid-ester (NHS-ester) moieties. Moreover, an enrichment step using liquid chromatography is usually performed after enzymatic digestion of the cross-linked proteins. Unfortunately, this approach suffers from several limitations. First, it requires large amounts of proteins. Second, NHS-ester cross-linkers are poorly efficient because of their fast hydrolysis in water. Fin…
Energy-energy correlations in hadronic final states from Z0 decays
1990
We have studied the energy-energy angular correlations in hadronic final states from Z0 decay using the DELPHI detector at LEP. From a comparison with Monte Carlo calculations based on the exact second order QCD matrix element and string fragmentation we find that Λ(5)/MS = 104-20 +25 (stat.)-20 +25(syst.)-00 +30(theor.) MeV, which corresponds to αs(91 GeV) = 0.106± 0.003 (stat.)±0.003(syst.)-0.000 +0.003(theor.). The theoretical error stems from different choices for the renormalization scale of αs. In the Monte Carlo simulation the scale of αs as well as the fragmentation parameters have been optimized to described reasonably well all aspects of multihadron production.
Effects of mutations of wild-type alpha-synuclein on its structure and dimerization and consequences for the Parkinson's disease : answers from coars…
2022
Alpha-synuclein is a protein of 140 amino acids, intrinsically disordered and found abundantly in the brain and whose toxic aggregation there is the hallmark of the Parkinson's disease.The aim of the thesis is to understand the effects of certain mutations on the dimerization of alpha-synuclein and to contribute to understand its involvement in Parkinson's disease by using coarse-grained molecular dynamics. Coarse-grained molecular dynamics trajectories were computed using the UNited-RESidue (UNRES) program and provided unprecedented sampling (over an effective time of the order of milliseconds) of the alpha-synuclein structures in monomeric and dimeric forms. These data were generated both…
Influence of ADAM10 on prion protein processing and scrapie infectiosity in vivo.
2009
Abstract Both the cellular prion protein (PrPc) and the amyloid precursor protein (APP) are physiologically subjected to complex proteolytic processing events. While for APP the proteinases involved – alpha-, beta- and gamma-secretase – have been identified in vitro and in vivo, the cleavage of PrPc by now has been linked only to the shedding activity of the metalloproteinase ADAM10 and/or ADAM17 in cell culture. Here we show that neuronal overexpression of the alpha-secretase ADAM10 in mice reduces all PrPc species detected in the brain instead of leading to enhanced amounts of specific cleavage products of PrPc. Additionally, the incubation time of mice after scrapie infection is signific…
Aggregation processes of intrinsically disordered proteins: influence of the environment
Energy dependence of the differences between the quark and gluon jet fragmentation
1996
Three jet events arising from decays of the Z beson, collected by the DELPHI detector, were used to measure differences in quark and gluon fragmentation. Gluon jets were anti-tagged by identifying b quark jets. Unbiased quark jets came from events with two jets plus one photon. Quark and gluon jet properties in different energy ranges were compared for the first time within the same detector. Quark and gluon jets of nearly the same energy in symmetric three jet event topologies were also compared. Using three independent methods, the average value of the ratio of the mean charged multiplicities of gluon and quark jets is [ r ] = 1.241 +/- 0.015 (stat.) +/- 0.025 (syst.). Gluon jets are broa…
Vulnerability of peripheral catecholaminergic neurons to MPTP is not regulated by alpha-synuclein.
2010
Although generally considered a prototypical movement disorder, Parkinson's disease is commonly associated with a broad-spectrum of non-motor symptoms, including autonomic dysfunctions caused by significant alterations in catecholaminergic neurons of the peripheral sympathetic nervous system. Here we present evidence that alpha-synuclein is highly expressed by sympathetic ganglion neurons throughout embryonic and postnatal life and that it is found in tyrosine hydroxylase-positive sympathetic fibers innervating the heart of adult mice. However, mice deficient in alpha-synuclein do not exhibit any apparent alterations in sympathetic development. Sympathetic neurons isolated from mouse embryo…
α-Synuclein expression levels do not significantly affect proteasome function and expression in mice and stably transfected PC12 cell lines
2004
α-Synuclein (α-syn) is a small protein of unknown function that is found aggregated in Lewy bodies, the histopathological hallmark of sporadic Parkinson disease and other synucleinopathies. Mutations in the α-syn gene and a triplication of its gene locus have been identified in early onset familial Parkinson disease. α-Syn turnover can be mediated by the proteasome pathway. A survey of published data may lead to the suggestion that overexpression of α-syn wild type, and/or their variants (A53T and A30P), may produce a decrease in proteasome activity and function, contributing to α-syn aggregation. To investigate the relationship between synuclein expression and proteasome function we have s…
Analyse et Estimations Spectrales des Processus alpha-Stables non-Stationnaires
2006
In this work a new spectral representation of a symmetric alpha-stable processes is introduced. It is based on a covariation pseudo-additivity and Morse-Transue's integral with respect to a bimesure built by using pseudo-additivity property. This representation, specific to S$\alpha$S processes, is analogous to the covariance of second order processes. On the other hand, it generalizes the representation established for stochastic integrals with respect to symmetric alpha-stable process of independent increments. We provide a classification of non-stationary harmonizable processes; this classification is based on the bimesure structure. In particular, we defined and investigated periodicall…