Search results for "ALPHA"
showing 10 items of 3228 documents
Hydroxylation of collagen type I: evidence that both lysyl and prolyl residues are overhydroxylated in osteogenesis imperfecta
1995
The composition of the collagens secreted into the media of fibroblast cultures of 39 patients with osteogenesis imperfecta (OI) was the same in controls and OI cultures. An abnormal migration pattern of collagens upon SDS-PAGE was evident in one third of the cultures investigated. Lysyl and prolyl hydroxylation of HPLC-purified alpha 1(I) chains was elevated in about 60% of cultures. The degree of hydroxylation was highest in the lethal forms. The extent of lysyl and prolyl hydroxylation showed a strong correlation (r = 0.74, P < 0.001). While high levels of hydroxylation are frequently observed in OI patients, a direct correlation between lysyl or prolyl hydroxylation and fracture rate or…
Construction and testing of the Moral Development Scale for Professionals (MDSP)
2010
Moral development among students is central for nursing education, because nursing is informed by moral ends, and it is essential that professional nurses have a well developed ability for moral behaviour, ethical reasoning and decision making. The aim of this study was to construct a new instrument for measuring moral development according to Kohlberg's theory of moral development among students and professionals, and initially test it for reliability and validity among students in professional education. Thirty-two items following the conventional and postconventional stages in the theory were developed. Different actions for reducing the items were implemented. The final version of the i…
Validation of the Dutch Eating Behavior Questionnaire (DEBQ) in a sample of Spanish women
2014
Contains fulltext : 124145.pdf (Publisher’s version ) (Closed access) The Dutch Eating Behavior Questionnaire (DEBQ) was developed to measure eating styles that may contribute to or attenuate the development of overweight. It comprises three scales that measure emotional, external and restrained eating. The main goal of this study is to evaluate the internal structure of the Spanish version of the DEBQ using updated psychometric techniques in a sample of women. A sample of 647 Spanish females answered the questionnaire. Both exploratory structural equation modeling and confirmatory factor analysis were used to evaluate the factor structure of the DEBQ. Reliabilities were estimated with Cron…
A brief form of the Perceived Social Support Questionnaire (F-SozU) was developed, validated, and standardized
2015
Abstract Objectives Development of a brief instrument (F-SozU K-6) for the measurement of perceived social support in epidemiologic contexts by shortening a well-established German questionnaire (F-SozU K-14). Study Design and Setting The development of the F-SozU K-6 consisted of two phases; phase 1: the F-SozU K-14 was presented to a general population sample representative for the Federal Republic of Germany (N = 2,007; age: 14–92 years). Six items for the short form were selected based on the maximization of coefficient alpha. Phase 2: the new short form (F-SozU K-6) was evaluated and standardized in an independent second population survey (N = 2,508, age: 14–92 years). Results The F-So…
Spanish Adaptation and Validation of the Short Internalized Homonegativity Scale (SIHS)
2016
Internalized homophobia has been related to mental health problems and sexual risk behaviors among nonheterosexual people. This article validates the Spanish adaptation of the Short Internalized Homonegativity Scale (SIHS). For this purpose, 347 men and 183 women completed the instrument. Exploratory factorial analysis showed three factors: public identification as homosexual (PIH), sexual comfort with homosexual people (SEXC), and social comfort with homosexual people (SOCC). These factors explained 57.96% of total variance. In addition, confirmatory factorial analysis supported this structure and internal consistency (Cronbach's alpha) was.80 for the full scale. The three subscales ranged…
Development and validation of the Barriers to Physical Activity and Sport Questionnaire for lesbian, gay, bisexual, transgender and queer/questioning…
2020
Abstract Objective The objective of the study was to develop the Barriers to Physical Activity and Sport Questionnaire for lesbian, gay, bisexual, transgender and queer/questioning (BPASQ-LGBTQ+ [encompassing all spectrums of sexuality and gender]) persons (BPASQ-LGBT), which measures barriers using a socio-ecological model, and to validate it through exploratory and confirmatory factor analysis. Study design Validation study. Methods First, content validity was achieved by (a) developing a bank of items, (b) discussing the adequacy of the items in a committee of experts and classifying the selected ones under three socio-ecological levels (intrapersonal, interpersonal, environmental) and (…
An assessment of prime‐boost vaccination schedules with AS03A‐adjuvanted prepandemic H5N1 vaccines: a randomized study in European adults
2012
Please cite this paper as: Gillard et al. (2012) An assessment of prime‐boost vaccination schedules with AS03A‐adjuvanted prepandemic H5N1 vaccines: a randomized study in European adults. Influenza and Other Respiratory Viruses DOI: 10.1111/j.1750‐2659.2012.00349.x. Background Long‐term persistence of immune response and safety of an H5N1 prepandemic influenza vaccine adjuvanted with AS03 (an α‐tocopherol oil‐in‐water emulsion‐based adjuvant system) was evaluated using various prime‐boost schedules that mimicked potential pandemic scenarios (NCT00430521). Methods Five hundred and twelve healthy adults aged 18–60 years received primary vaccination with one or two doses (0, 21 days schedule…
A genetic basis for IFN-gamma production and T-bet expression in humans.
2005
Abstract Th1 and Th2 cytokines secreted by polarized effector T cells play a pivotal role in the development of autoimmune and allergic diseases. However, the genetic basis of cytokine production by T lymphocytes in humans is poorly understood. In this study, we investigated the genetic contribution to cytokine production and regulation of T cell-specific transcription factors in a prospective twin study. We found a substantial genetic contribution to the production of Th1 cytokines such as IFN-γ and TNF-α with heritabilities of 0.85 (95% confidence intervals, 0.74–0.95) and 0.72 (0.50–0.93), respectively, whereas no genetic influence on production of the Th2 signature cytokine IL-4 was obs…
β2-adrenoreceptors control human skin microvascular reactivity.
2021
Topical α1- and α2-adrenoreceptor (ADRA1 and 2) agonists are effective in alleviating permanent vasodilation and facial erythema associated with rosacea by inducing skin vasoconstriction. Although β-adrenoreceptor (ADRB) antagonists are used off-label for rosacea, pharmacological and pharmacodynamic data pertaining to these receptors in skin micro-vessels are lacking. Objectives: To analyse the expression of different adrenergic receptors and their contribution to vasoreactivity in skin micro-vessels. Small arteries (500-800 μm) and arterioles (<200 μm) were studied in human foreskin tissue. Specifically, ADR-A1, -A2, -B1 and -B2 expression was assayed by immunofluorescence, polymerase chai…
Analysis of Candidate Genes in Celiac Disease: A Tool to Identify Life-Threatening Associated Genes?
2006
The authors have recently reported that celiac patients show a proinflammatory cytokine genetic profile characterized by the contemporaneous presence of both the tumour necrosis factor-alpha-308A and the interferon-gamma +874T allele-positive genotypes. The same alleles are considered risk factors for aging associated disease, whereas an anti-inflammatory cytokine genotype profile might be associated with an extended life expectancy. This paper reports data on the 1249-1250InsACAA/Non-Ins transforming growth factor (TGF)-beta2, a multifunctional anti-inflammatory cytokine, polymorphism distribution in 88 celiac disease (CD) patients, 99 age- and sex-matched controls, and 2895-year-old healt…