Search results for "ARX"

Valorisation of Dairy Wastes Through Kefir Grain Production

2019

The main aim of this work was to investigate on kefir grains (KGs) increase using different dairy by-products. To this purpose, whey and deproteinized whey from cow, goat and sheep milk were compared to the pasteurized milk of the corresponding animal species. Each substrate was inoculated with 3% (w/v) of KGs cultivated in ultra-high temperature cow milk and evalu- ated for pH decrease, total titratable acidity increase and development of lactic acid bacteria (LAB) and yeasts released in the matrices after 24 h incubation at three different temperatures (20, 25 and 30 °C). The genotypic characterization of the dominating microbial populations resulted in the identification of two LAB belon…

Use of Kluyveromyces marxianus to Increase Free Monoterpenes and Aliphatic Esters in White Wines

2021

An increasing interest in novel wine productions is focused on non-Saccharomyces yeasts due to their potential in improving sensory profiles. Although Kluyveromyces marxianus has been originally isolated from grapes and its enzymatic activities are used in oenology, rarely it has been used as co-starter. The K. marxianus Km L2009 strain has been characterized here and selected as a co-starter both at laboratory- and winery-scale fermentation. The Km L2009 strain showed growth of up to 40 (mg/L) of sulfites and 6% (v/v) of ethanol. Gas chromatographic analysis demonstrates that wines produced by mixed fermentation contain remarkably higher quantities of free monoterpenes and aliphatic esters…

Optimization of Synthetic Media Composition for Kluyveromyces marxianus Fed-Batch Cultivation

2021

The Kluyveromyces marxianus yeast recently has gained considerable attention due to its applicability in high-value-added product manufacturing. In order to intensify the biosynthesis rate of a target product, reaching high biomass concentrations in the reaction medium is mandatory. Fed-batch processes are an attractive and efficient way how to achieve high cell densities. However, depending on the physiology of the particular microbial strain, an optimal media composition should be used to avoid by-product synthesis and, subsequently, a decrease in overall process effi-ciency. Thus, the aim of the present study was to optimise the synthetic growth medium and feeding solution compositions (…

Experimental trade-offs between different strategies for multihop communications evaluated over real deployments of wireless sensor network for envir…

2018

Although much work has been done since wireless sensor networks appeared, there is not a great deal of information available on real deployments that incorporate basic features associated with these networks, in particular multihop routing and long lifetimes features. In this article, an environmental monitoring application (Internet of Things oriented) is described, where temperature and relative humidity samples are taken by each mote at a rate of 2 samples/min and sent to a sink using multihop routing. Our goal is to analyse the different strategies to gather the information from the different motes in this context. The trade-offs between ‘sending always’ and ‘buffering locally’ approac…

Underwater Wireless Communications for Cooperative Robotics with UWSim-NET

2019

The increasing number of autonomous underwater vehicles (AUVs) cooperating in underwater operations has motivated the use of wireless communications. Their modeling can minimize the impact of their limited performance in real-time robotic interventions. However, robotic frameworks hardly ever consider the communications, and network simulators are not suitable for HIL experiments. In this work, the UWSim-NET is presented, an open source tool to simulate the impact of communications in underwater robotics. It gathers the benefits of NS3 in modeling communication networks with those of the underwater robot simulator (UWSim) and the robot operating system (ROS) in modeling robotic systems. Thi…

Isolation, identification and oenological characterization of non-Saccharomyces yeasts in a Mediterranean island

2016

UNLABELLED We isolated, identified and characterized yeast strains from grapes, and their fermented musts, sampled in the small island of Linosa, where there are no wineries and therefore the possibility of territory contamination by industrial strains is minimal. By traditional culture-dependent methods, we isolated 3805 colonies, distinguished by molecular methods in 17 different species. Five hundred and forty-four isolates were analysed for the main oenological characteristics such as fermentative vigour with and without sulphites, sugar consumption and production of alcohol, volatile acidity, hydrogen sulphide, glycerol and β-glucosidase. This analysis identified Kluyveromyces marxianu…

Probiotic yeast Kluyveromyces marxianus CIDCA 8154 shows anti-inflammatory and anti-oxidative stress properties in in vivo models.

2015

Inflammatory bowel diseases (IBDs) are complex affections with increasing incidence worldwide. Multiple factors are involved in the development and maintenance of the symptoms including enhanced oxidative stress in intestinal mucosa. The conventional therapeutic approaches for IBDs are based on the use anti-inflammatory drugs with important collateral effects and partial efficacy. In the present work we tested the anti-inflammatory capacity of Kluyveromyces marxianus CIDCA 8154 in different models. In vitro, we showed that the pretreatment of epithelial cells with the yeast reduce the levels of intracellular reactive oxygen species. Furthermore, in a murine model of trinitro benzene sulfon…

Synchronized Activity in The Main and Accessory Olfactory Bulbs and Vomeronasal Amygdala Elicited by Chemical Signals in Freely Behaving Mice

2017

AbstractChemosensory processing in mammals involves the olfactory and vomeronasal systems, but how the activity of both circuits is integrated is unknown. In our study, we recorded the electrophysiological activity in the olfactory bulbs and the vomeronasal amygdala in freely behaving mice exploring a battery of neutral and conspecific stimuli. The exploration of stimuli, including a neutral stimulus, induced synchronic activity in the olfactory bulbs characterized by a dominant theta rhythmicity, with specific theta-gamma coupling, distinguishing between vomeronasal and olfactory structures. The correlated activation of the bulbs suggests a coupling between the stimuli internalization in t…

Economic Socialization: Childhood, Adolescence, and Early Adulthood

2017

Constitución y verdad. La controversia entre Rafael de Vélez y Joaquín Lorenzo Villanueva a propósito de la <em>Apología del Trono</em>

2017

El trabajo aborda el lugar del discurso antiliberal reaccionario en los primeros años de la Revolución en España. Se centra para ello en la controversia que mantuvieron el diputado liberal Joaquín Lorenzo Villanueva y el publicista y fraile capuchino Rafael de Vélez entre 1820 y 1825. Las fuentes utilizadas remiten directamente a los escritos de ambos polemistas tomando como punto de partida la «Apología del Trono» del Padre Vélez. Metodológicamente, la perspectiva de análisis adoptada parte de la consideración teórica de que la crítica al liberalismo, en sus diversas manifestaciones, fue parte inherente al proceso revolucionario y no un elemento marginal del mismo. El examen confrontado de…