Search results for "ASTER"

showing 10 items of 2223 documents

NOseq: amplicon sequencing evaluation method for RNA m6A sites after chemical deamination

2020

Abstract Methods for the detection of m6A by RNA-Seq technologies are increasingly sought after. We here present NOseq, a method to detect m6A residues in defined amplicons by virtue of their resistance to chemical deamination, effected by nitrous acid. Partial deamination in NOseq affects all exocyclic amino groups present in nucleobases and thus also changes sequence information. The method uses a mapping algorithm specifically adapted to the sequence degeneration caused by deamination events. Thus, m6A sites with partial modification levels of ∼50% were detected in defined amplicons, and this threshold can be lowered to ∼10% by combination with m6A immunoprecipitation. NOseq faithfully d…

AdenosineSequence analysisAcademicSubjects/SCI00010Bisulfite sequencingDeaminationAdenosine/analogs & derivatives; Adenosine/analysis; Algorithms; Animals; Chromatography Liquid; Deamination; Drosophila melanogaster/genetics; HEK293 Cells; HeLa Cells; High-Throughput Nucleotide Sequencing/methods; Humans; RNA/chemistry; RNA Long Noncoding/chemistry; RNA Messenger/chemistry; RNA Ribosomal 18S/chemistry; Sequence Alignment; Sequence Analysis RNA/methods; Tandem Mass SpectrometrySequence alignmentComputational biologyBiology010402 general chemistry[SDV.BBM.BM] Life Sciences [q-bio]/Biochemistry Molecular Biology/Molecular biology01 natural sciencesTranscriptome03 medical and health sciencesNarese/13Tandem Mass Spectrometry[SDV.BBM.GTP]Life Sciences [q-bio]/Biochemistry Molecular Biology/Genomics [q-bio.GN]GeneticsRNA Ribosomal 18SAnimalsHumansRNA MessengerComputingMilieux_MISCELLANEOUS030304 developmental biology0303 health sciencesSequence Analysis RNARNAHigh-Throughput Nucleotide Sequencing[SDV.BBM.BM]Life Sciences [q-bio]/Biochemistry Molecular Biology/Molecular biologyAmpliconRibosomal RNA0104 chemical sciencesDrosophila melanogasterHEK293 CellsDeaminationMethods OnlineRNA[SDV.BBM.GTP] Life Sciences [q-bio]/Biochemistry Molecular Biology/Genomics [q-bio.GN]RNA Long NoncodingSequence AlignmentAlgorithmsChromatography LiquidHeLa Cells
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Testing the Cross-Cultural Robustness of the Minority Stress Model in Gay and Bisexual Men

2017

The study tests the cross-cultural robustness of the minority stress model using a most different systems approach. Data from Western European, Eastern European (including Russian), Indian, Philippine, and Thai gay and bisexual men was obtained online. The final sample consisted of N = 90,467 participants who reported their level of satisfaction with life, victimization, felt stigma, and internalized homonegativity, as well as their disclosure status and sociodemographic details. Stepwise linear regressions were conducted on reported levels of satisfaction with life. Higher levels of victimization, felt stigma, and internalized homonegativity predicted lower satisfaction with life in all gr…

AdultCross-Cultural ComparisonMaleAsiaSocial PsychologySexual BehaviorEmotionsSocial Stigma050109 social psychologyEducationGender StudiesSexual and Gender Minorities03 medical and health sciencesHumansCross-cultural0501 psychology and cognitive sciencesSociologyHomosexuality MaleRobustness (economics)Crime VictimsGeneral PsychologyDefense Mechanisms030505 public health05 social sciencesGeneral MedicineCross-cultural studiesMinority stressEuropeEastern europeanWestern europeBisexualityDemographic economics0305 other medical scienceStress PsychologicalJournal of Homosexuality
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Radiation dose distribution in functional heart regions from tangential breast cancer radiotherapy

2015

Abstract Background and purpose To analyze the distribution of individually-determined radiation dose to the heart and its functional sub-structures after radiotherapy in breast cancer patients treated in Germany during 1998–2008. Material and methods We obtained electronic treatment planning records for 769 female breast cancer patients treated with megavoltage tangential field radiotherapy. All dose distributions were re-calculated using Eclipse with the anisotropic analytical algorithm (AAA) for photon fields, and the electron Monte Carlo algorithm for electron boost fields. Based on individual dose volume histograms for the complete heart and several functional sub-structures, we estima…

AdultDose-volume histogrammedicine.medical_treatmentBreast Neoplasms030218 nuclear medicine & medical imaging03 medical and health sciencesQuadrant (abdomen)0302 clinical medicineBreast cancerHumansMedicineDosimetryRadiology Nuclear Medicine and imagingBreastRadiation treatment planningAgedbusiness.industryRadiotherapy Planning Computer-AssistedHeartRadiotherapy DosageHematologyMiddle Agedmedicine.diseaseRadiation therapyOncologyParasternal line030220 oncology & carcinogenesisFemalebusinessNuclear medicineMonte Carlo MethodBody mass indexRadiotherapy and Oncology
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Barriers and motivators for uptake of cervical cancer prevention strategies in Eastern Europe: Perspective from Latvia

2019

Introduction The incidence of cervical cancer (CC) in eastern European countries is high; however, uptake of available prevention strategies is suboptimal. We explored knowledge, behaviors, and attitudes toward CC prevention strategies in Latvian women. Methods A mixed-methods study of Latvian women, consisting of surveys and semi-structured interviews, was conducted in Riga, Latvia in September and October 2015. Results Altogether, 158 surveys were completed and 10 interviews were conducted. In total, 87% (n = 135) had previously had a smear test, and 67% (n = 105) correctly identified that cervical cytology was performed to identify precancerous changes. The practice of annual gynecologic…

AdultHealth Knowledge Attitudes Practicemedicine.medical_specialtymedia_common.quotation_subjectUterine Cervical NeoplasmsShameBFDermatologyCohort StudiesRC0254Young AdultRA0421HumansMedicineEurope EasternYoung adultAgedmedia_commonCervical cancerMotivationCervical screeningbusiness.industryIncidence (epidemiology)LatvianMiddle AgedPatient Acceptance of Health Caremedicine.diseaseLatvialanguage.human_languageEastern europeanInfectious DiseasesFamily medicinelanguageFemalebusinessCohort study
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Effects of a trail running competition on muscular performance and efficiency in well-trained young and master athletes

2010

International audience; To determine the acute effects of a trail running competition and the age-dependent differences between young and master athletes, 23 subjects [10 young (30.5 ± 7 years), 13 master (45.9 ± 5.9 years)] participated in a 55-km trail running competition. The study was conceived as an intervention study compromising pre, post 1, 24, 48 and 72 h measurements. Measurements consisted of blood tests, ergometer cycling and maximal isometric voluntary contractions (MVC). Parameters monitored included MVC, twitch-and M-wave properties, EMG (RMS) of the vastus lateralis, two locomotion efficiency calculations and muscle damage markers in the blood (CK, LDH). Results indicate pos…

AdultMaleAcute effectsCompetitive Behaviormedicine.medical_specialtyPhysiology[SHS.SPORT.PS] Humanities and Social Sciences/Sport/Sport physiology[SHS.SPORT.PS]Humanities and Social Sciences/Sport/Sport physiologyEfficiencyIsometric exerciseAthletic PerformanceMuscle damage[ SHS.SPORT ] Humanities and Social Sciences/SportRunningYoung Adult03 medical and health sciencesProfessional Competence0302 clinical medicinePhysical medicine and rehabilitationTrail running / Ultra long distance /Master athlete / Eccentric contractions / Muscle damage / EfficiencyPhysiology (medical)HumansMedicineEccentricOrthopedics and Sports MedicineMuscle SkeletalContraction time[SHS.SPORT]Humanities and Social Sciences/Sportbiologybusiness.industryAthletesAge FactorsPublic Health Environmental and Occupational Health[ SHS.SPORT.PS ] Humanities and Social Sciences/Sport/Sport physiology[SHS.SPORT] Humanities and Social Sciences/Sport030229 sport sciencesGeneral MedicineMiddle Agedbiology.organism_classificationIntervention studiesAthletesPhysical FitnessPhysical EndurancePhysical therapyFemalemedicine.symptombusiness030217 neurology & neurosurgeryMuscle contraction
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Effects of achievement goals on perceptions of competence in conditions of unfavourable social comparisons: The mastery goal advantage effect

2017

Background Previous prospective studies have documented that mastery-approach goals are adaptive because they facilitate less negative psychological responses to unfavourable social comparisons than performance-approach goals. Aims This study aimed to confirm this so-called ‘mastery goal advantage’ effect experimentally. Methods A 2 × 3 design was adopted where achievement goals (mastery vs. performance) and normative information (favourable vs. no-normative information vs. unfavourable) were manipulated as between participant factors. Sample Participants were 201 undergraduates, 57 males and 144 females, ranging in age from 17 to 55 years (Mage = 22.53, SD = 6.51). Results Regression analy…

AdultMaleAdolescentUniversitiesmedia_common.quotation_subjectmastery goal advantage effect050109 social psychologyAcademic achievementEducationYoung AdultSocial cognitionachievement goalsDevelopmental and Educational PsychologyHumansta5160501 psychology and cognitive sciencesStudentsta515media_commonSocial comparison theoryAcademic SuccessGoal orientation05 social sciences050301 educationMastery learningMiddle AgedSelf Efficacyunfavourable social comparisonsSocial Perceptionperceptions of competenceWell-beingHappinessFemaleSocial competencePsychologyGoals0503 educationSocial psychologyBritish Journal of Educational Psychology
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Perceived Racial/Ethnic Discrimination and Psychological Outcomes Among Adult International Adoptees in Finland: Moderating Effects of Social Support…

2015

Quantitative literature on international adoptees and racial/ethnic discrimination is lacking despite results in qualitative studies from Europe and the United States that have consistently indicated how racism constantly complicates adoptees' everyday lives. To advance the literature, the present study examined the prevalence of perceived racial/ethnic discrimination among 213 adult international adoptees in Finland (59.6% women and 40.4% men, mean age 24.1 years), and the association between perceived racial/ethnic discrimination and psychological well-being indicators, including psychological distress and sleeping problems. In addition, we examined social support and sense of coherence a…

AdultMaleAsiaSense of Coherencemedia_common.quotation_subjectinternational adoptionPsycINFOsense of coherenceRacismDevelopmental psychologySocial supportYoung AdultRacismArts and Humanities (miscellaneous)psychological distresssleeping problemsAdoptionDevelopmental and Educational PsychologyHumansta516Europe EasternYoung adultAssociation (psychology)ta515Finlandmedia_commonSocial Supportracial/ethnic discriminationSouth AmericaMental healthta3123Psychiatry and Mental healthDistressta5141AfricaFemalePsychology (miscellaneous)PsychologyStress PsychologicalQualitative researchAmerican Journal of Orthopsychiatry
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Clock genes beyond the clock: CLOCK genotype biases neural correlates of moral valence decision in depressed patients

2007

Gene polymorphisms in the mammalian biological clock system influence individual rhythms. A single nucleotide polymorphism (SNP) in the 3' flanking region of CLOCK (3111 T/C; rs1801260) influenced diurnal preference in healthy humans and caused sleep phase delay and insomnia in patients affected by bipolar disorder. Genes of the biological clock are expressed in many brain structures other than in the 'master clock' suprachiasmatic nuclei. These areas, such as cingulate cortex, are involved in the control of many human behaviors. Clock genes could then bias 'nonclock' functions such as information processing and decision making. Thirty inpatients affected by a major depressive episode under…

AdultMaleCingulate cortexGenotypeDecision MakingCLOCK ProteinsMotor ActivityNeuropsychological TestsMoralsGyrus CinguliDevelopmental psychologyArousalBehavioral NeuroscienceImage Processing Computer-AssistedGeneticsmedicineHumansCircadian rhythmAllelesAgedDepressive Disorder MajorNeural correlates of consciousnessmedicine.diagnostic_testGenetic Carrier ScreeningHomozygoteNeuropsychologyMiddle AgedImage EnhancementMagnetic Resonance ImagingCircadian RhythmSemanticsOxygenCLOCKNeurologyTrans-ActivatorsFemaleMaster clockArousalFunctional magnetic resonance imagingPsychologyNeuroscienceGenes, Brain and Behavior
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Psychometric properties of the Haitian Creole version of the Resilience Scale with a sample of adult survivors of the 2010 earthquake

2015

Abstract Background Resilience is defined as the ability of people to cope with disasters and significant life adversities. The present paper aims to investigate the underlying structure of the Creole version of the Resilience Scale and its psychometric properties using a sample of adult survivors of the 2010 earthquake. Methods A parallel analysis was conducted to determine the number of factors to extract and confirmatory factor analysis was performed using a sample of 1355 adult survivors of the 2010 earthquake from people of specific places where earthquake occurred with an average age of 31.57 (SD = 14.42). All participants completed the Creole version of Resilience Scale (RS), the Imp…

AdultMaleGerontologyAdolescentPsychometricslcsh:RC435-571media_common.quotation_subjectPoison controlDisastersStress Disorders Post-TraumaticYoung AdultSocial supportHaitian CreoleCronbach's alphalcsh:PsychiatrySurveys and QuestionnairesEarthquakesHumansSurvivorsAgedmedia_commonAged 80 and overPsychiatric Status Rating ScalesDepressionBeck Depression InventorySocial SupportConstruct validityMiddle AgedResilience PsychologicalHaitilanguage.human_languageConfirmatory factor analysisPsychiatry and Mental healthClinical PsychologylanguageFemalePsychological resiliencePsychologyClinical psychologyComprehensive Psychiatry
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Socioeconomic inequalities in injury mortality in small areas of 15 European cities.

2013

This study analysed socioeconomic inequalities in mortality due to injuries in small areas of 15 European cities, by sex, at the beginning of this century. A cross-sectional ecological study with units of analysis being small areas within 15 European cities was conducted. Relative risks of injury mortality associated with the socioeconomic deprivation index were estimated using hierarchical Bayesian model. The number of small areas varies from 17 in Bratislava to 2666 in Turin. The median population per small area varies by city (e.g. Turin had 274 inhabitants per area while Budapest had 76,970). Socioeconomic inequalities in all injury mortality are observed in the majority of cities and a…

AdultMaleHealth (social science)AdolescentUrban PopulationGeography Planning and DevelopmentPopulationPoison controlSocial class03 medical and health sciencesYoung Adult0302 clinical medicineEnvironmental healthInjury preventionHumansSocioeconomic inequalities030212 general & internal medicineMortalityHealthcare DisparitieseducationSocioeconomicsUrban areasSocioeconomic statusSmall-Area Analysiseducation.field_of_study030505 public healthInjuries1. No povertyPublic Health Environmental and Occupational HealthEcological studyMiddle Aged3. Good healthEastern europeanEuropeGeographySmall areasCross-Sectional StudiesSocial ClassSmall-Area AnalysisWounds and InjuriesFemale0305 other medical scienceHealthplace
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