Search results for "ATS"

Expression of glial filament protein (GFP) in nerve sheaths and non-neural cells re-examined using monoclonal antibodies, with special emphasis on th…

1986

We describe two novel monoclonal antibodies specific for glial filament protein (GFP), i.e., GF12.23 and GF12.24 (both IgG2a]. These cross-react over a broad range of species with epitopes located in the alpha-helical rod domain typical of all intermediate filament (IF) proteins. These monoclonal antibodies were used, in conjunction with other monoclonal GFP antibodies, rabbit antiserum to GFP, and various antibodies to other cytoskeletal proteins, to examine the occurrence of GFP in cells outside of the central nervous system of rodents, cows, and humans. We detected some scattered GFP-containing cells in the neural sheaths in some species but not in others, and we obtained different resul…

Pharmacological analysis of intrinsic neural control of rat duodenum motility in vitro

1988

Physiological and metabolic actions of mycophenolate mofetil on cultured newborn rat cardiomyocytes in normoxia and in simulated ischemia

2004

Mycophenolate mofetil (MMF) is a new immunosuppressive drug used to reduce acute rejection after heart transplantation. As with other immunosuppressive drugs, MMF therapy is associated with several adverse effects. However, the direct effects of MMF on myocardial tissue has not been yet evaluated. The aim of the work was thus to evaluate the effects of MMF on isolated cardiomyocytes (CM) in normal conditions and in an in vitro model of simulated ischemia (SI; substrate-free hypoxia) and reperfusion (R; reoxygenation). Myocyte-enriched cultures were prepared from newborn rat heart ventricles. The transmembrane potentials were recorded using conventional microelectrodes and the cell contracti…

El régimen local tras el fracaso de la reforma de 2013

2017

La reforma de 2013 ha sido, incluso tras los diluidos planteamientos respecto de lo que fueron algunos de sus objetivos iniciales con que quedó plasmada legalmente, el mayor intento de reforma del régimen local español desde su implantación en los per les que actualmente conocemos con la Ley Reguladora de las Bases del Régimen Local de 1985 (LRBRL). Podemos, sin embargo, considerar que en todos y cada uno de los referidos ejes esenciales las orientaciones de la misma, casi tres años después de su aprobación, se han manifestado como fracasadas, bien por la incorrecta evaluación de la efectiva necesidad de una reforma en ese punto, bien por la incapacidad de la norma para impulsar cambios efe…

Los deberes de los administradores en situación de insolvencia inminente en la propuesta de Directiva sobre marcos de reestructuración.

2019

La última versión de la Propuesta de Directiva sobre marcos de reestructuración preventiva de 28 de marzo de 2019, ha recuperado en su art. 19 la obligación de los administradores de tomar en consideración el interés de los acreedores en las situaciones de insolvencia inminente. Dicha obligación supone una modulación del contenido del interés social en dicha situación, el cual junto al interés de los socios, comprende también el interés de los acreedores. Esta configuración compleja del interés social obliga por tanto a analizar cuál es el concreto contenido de los deberes de los administradores en dicha situación de insolvencia inminente, habida cuenta de su orientación hacia la consecució…

Tiesnešu atsevišķās domas kā tiesību palīgavots. Augstākās tiesas Administratīvo lietu departamenta pieredze (2014.-2017.)

2018

Bakalaura darba tēmas nosaukums ir „Tiesnešu atsevišķās domas kā tiesību palīgavots. Augstākās tiesas Administratīvo lietu departamenta pieredze (2014.-2017.) ”. Tā kā tiesnešu atsevišķās domas nav bieži sastopamas, šī darba mērķis ir noskaidrot kādu ietekmi sniedz tiesneša atsevišķo domu institūts. Lai to noskaidrotu, darba autore analizē tiesību zinātnieku dažādos viedokļus par tiesnešu atsevišķo domu institūta esību. Autore klasificē tiesneša atsevišķo domu iedalījuma kritērijus un aplūko tiesnesim piešķirtās brīvības, vārda brīvību un neatkarību, tiesneša atsevišķo domu izpratnē. Salīdzinošā nolūkā tiek apskatīts ne tikai Latvijas, bet arī citu valstu normatīvais regulējums. Darba autor…

Neuroimmune Activation and Myelin Changes in Adolescent Rats Exposed to High-Dose Alcohol and Associated Cognitive Dysfunction: A Review with Referen…

2014

Aims: The aim of the study was to assess whether intermittent ethanol administration to adolescent rats activates innate immune response and TLRs signalling causing myelin disruption and long-term cognitive and behavioural deficits. Methods: We used a rat model of intermittent binge-like ethanol exposure during adolescence. Results: Binge-like ethanol administration to adolescent rats increased the gene expression of TLR4 and TLR2 in the prefrontal cortex (PFC), as well as inflammatory cytokines TNF alpha and IL-1 beta. Up-regulation of TLRs and inflammatory mediators were linked with alterations in the levels of several myelin proteins in the PFC of adolescent rats. These events were assoc…

Induction of the anti-ergotypic response.

1993

The injection of syngeneic activated T cells into rodents can induce a T cell response against activation markers of the T cells, ergotopes. The responding anti-ergotypic T cells have been shown to suppress experimental autoimmune encephalomyelitis (EAE). This paper reports the characteristics of the anti-ergotypic response. It was found that irradiated activated T cells were as good as untreated living activated T cells in inducing anti-ergotypic cells in vivo. Glutardialdehyde-fixed (0.3%) cells were poor stimulators in vivo and non-stimulatory in vitro. Dilution of glutardialdehyde to 0.003% before fixation preserved the stimulatory capacity in vitro. Fixation or irradiation of T cells a…

Lack of requirement for CD8+ cells in recovery from and resistance to experimental autoimmune encephalomyelitis.

1995

Abstract Experimental autoimmune encephalomyelitis (EAE) is a model of T-cell mediated autoimmune disease. Active disease is mediated by myelin basic protein specific CD4+T-cells, whose adoptive transfer can also induce passive disease. In the Lewis rat EAE is a transient disease inducing lasting resistance to rechallenge. The mechanisms of recovery and resistance are poorly understood. CD8+suppressor T-cells have mostly been thought to be central, especially in resistance to reinduction of the disease. In this study we showed by complete depletion of CD8+cells that this subset does not influence either recovery or resistance to EAE in the Lewis rat. This was further confirmed by depleting …

Evaluation of the stromal vascular fraction of adipose tissue as the basis for a stem cell-based tissue-engineered vascular graft

2017

Abstract Objective One of the rate-limiting barriers within the field of vascular tissue engineering is the lengthy fabrication time associated with expanding appropriate cell types in culture. One particularly attractive cell type for this purpose is the adipose-derived mesenchymal stem cell (AD-MSC), which is abundant and easily harvested from liposuction procedures. Even this cell type has its drawbacks, however, including the required culture period for expansion, which could pose risks of cellular transformation or contamination. Eliminating culture entirely would be ideal to avoid these concerns. In this study, we used the raw population of cells obtained after digestion of human lipo…