Search results for "ATTAC"

The emotional timeline of September 11, 2001.

2010

Persistent episomal transgene expression in liver following delivery of a scaffold/matrix attachment region containing non-viral vector

2008

An ideal gene therapy vector should enable persistent transgene expression without limitations of safety and reproducibility. Here we report the development of a non-viral episomal plasmid DNA (pDNA) vector that appears to fulfil these criteria. This pDNA vector combines a scaffold/matrix attachment region (S/MAR) with a human liver-specific promoter (alpha1-antitrypsin (AAT)) in such a way that long-term expression is enabled in murine liver following hydrodynamic injection. Long-term expression is demonstrated by monitoring the longitudinal luciferase expression profile for up to 6 months by means of in situ bioluminescent imaging. All relevant control pDNA constructs expressing luciferas…

The Influence of Place Attachment, Ad-Evoked Positive Affect, and Motivation on Intention to Visit: Imagination Proclivity as a Moderator

2019

Integrating the theoretical foundations of symbolic interactionism, parasocial interaction, direct affect transfer, push and pull motivational framework, and narrative transportation, this study investigates the determinants of tourists’ intention to visit a destination using Heidi, a famous literary and television series persona, as a stimulus. Place attachment, ad-evoked positive affect, and motivation were theorized as antecedents of intention to visit and imagination proclivity as a moderator. The model was tested using data collected from 410 prospective Spanish tourists. Results show motivation and place attachment as key determinants of intention to visit among individuals with high…

Development of S/MAR minicircles for enhanced and persistent transgene expression in the mouse liver.

2010

We have previously described the development of a scaffold/matrix attachment region (S/MAR) episomal vector system for in vivo application and demonstrated its utility to sustain transgene expression in the mouse liver for at least 6 months following a single administration. Subsequently, we observed that transgene expression is sustained for the lifetime of the animal. The level of expression, however, does drop appreciably over time. We hypothesised that by eliminating the bacterial components in our vectors, we could improve their performance since bacterial sequences have been shown to be responsible for the immunotoxicity of the vector and the silencing of its expression when applied i…

Data Mining Approach for Detection of DDoS Attacks Utilizing SSL/TLS Protocol

2015

Denial of Service attacks remain one of the most serious threats to the Internet nowadays. In this study, we propose an algorithm for detection of Denial of Service attacks that utilize SSL/TLS protocol. These protocols encrypt the data of network connections on the application layer which makes it impossible to detect attackers activity based on the analysis of packet payload. For this reason, we concentrate on statistics that can be extracted from packet headers. Based on these statistics, we build a model of normal user behavior by using several data mining algorithms. Once the model has been built, it is used to detect DoS attacks. The proposed framework is tested on the data obtained w…

Antecedentes y consecuencias del uso de live videos en el marketing de influencers

2023

Desde la existencia de los medios de comunicación masiva, esto es, prensa, radio, televisión e internet, existen líderes de opinión que influyen en la toma de decisiones de su audiencia. Con la adopción masiva de las plataformas de redes sociales, la ubicuidad que proporcionan los dispositivos móviles y los live videos, la barrera de información entre el líder de opinión y su audiencia se ha reducido significativamente (Carillo et al., 2017). Las empresas, al entender que el desplazamiento de la atención de los consumidores hacia lo digital es más permanente que transitorio, han destinado mayores recursos a la publicidad digital, particularmente, en el influencer marketing o marketing de in…

Parenting, Peer Relationships, Academic Self-efficacy, and Academic Achievement: Direct and Mediating Effects

2017

The aim of the present study is to analyze the relation between authoritative and permissive parenting styles with the kinds of adolescent peer relationships (attachment, victimization, or aggression), and of the latter ones, in turn, with academic self-efficacy, and academic performance, in three waves that range from the early-mid adolescence to late adolescence. Five hundred Spanish adolescents, of both sexes, participated in a three-wave longitudinal study in Valencia, Spain. In the first wave, adolescents were either in the third year of secondary school or the fourth year of secondary school. The mean age in the first wave was 14.70 (SD = 0.68; range = 13–16 years). Child Report of Pa…

I hate my workplace but I am very attached to it: workplace attachment style

2017

Purpose The purpose of this paper is to investigate whether different patterns of workplace attachment exist and to explore the relations between adult attachment styles and the level of workplace attachment. Design/methodology/approach Participants were 351 Italian employees who completed a questionnaire composed of the Workplace Attachment Scale and the Relationship Questionnaire. Data were analyzed using correspondence analysis. Findings The results showed that high scores on workplace attachment correlated significantly with secure attachment style, while low scores correlated with insecure attachment styles. These results shed light on different workplace attachment styles. Research l…

On the pathogenesis of atherosclerosis: enzymatic transformation of human low density lipoprotein to an atherogenic moiety.

1995

Combined treatment with trypsin, cholesterol esterase, and neuraminidase transforms LDL, but not HDL or VLDL, to particles with properties akin to those of lipid extracted from atherosclerotic lesions. Single or double enzyme modifications, or treatment with phospholipase C, or simple vortexing are ineffective. Triple enzyme treatment disrupts the ordered and uniform structure of LDL particles, and gives rise to the formation of inhomogeneous lipid droplets 10-200 nm in diameter with a pronounced net negative charge, but lacking significant amounts of oxidized lipid. Enzymatically modified LDL (E-LDL), but not oxidatively modified LDL (ox-LDL), is endowed with potent complement-activating c…

Absolute Humidity Influences the Seasonal Persistence and Infectivity of Human Norovirus

2014

ABSTRACT Norovirus (NoV) is one of the main causative agents of acute gastroenteritis worldwide. In temperate climates, outbreaks peak during the winter season. The mechanism by which climatic factors influence the occurrence of NoV outbreaks is unknown. We hypothesized that humidity is linked to NoV seasonality. Human NoV is not cultivatable, so we used cultivatable murine norovirus (MNV) as a surrogate to study its persistence when exposed to various levels of relative humidity (RH) from low (10% RH) to saturated (100% RH) conditions at 9 and 25°C. In addition, we conducted similar experiments with virus-like particles (VLPs) from the predominant GII-4 norovirus and studied changes in bin…