Search results for "Actor"
showing 10 items of 19159 documents
Gene regulation in parthenocarpic tomato fruit.
2009
Parthenocarpy is potentially a desirable trait for many commercially grown fruits if undesirable changes to structure, flavour, or nutrition can be avoided. Parthenocarpic transgenic tomato plants (cv MicroTom) were obtained by the regulation of genes for auxin synthesis (iaaM) or responsiveness (rolB) driven by DefH9 or the INNER NO OUTER (INO) promoter from Arabidopsis thaliana. Fruits at a breaker stage were analysed at a transcriptomic and metabolomic level using microarrays, real-time reverse transcription-polymerase chain reaction (RT-PCR) and a Pegasus III TOF (time of flight) mass spectrometer. Although differences were observed in the shape of fully ripe fruits, no clear correlatio…
Purification and characterization of geranyl diphosphate synthase from Vitis vinifera L. cv Muscat de Frontignant cell cultures
1993
A geranyl diphosphate synthase (EC 2.5.1.1), which catalyzes the formation of geranyl diphosphate from dimethylallyl diphosphate and isopentenyl diphosphate, was isolated from Vitis vinifera L. cv Muscat de Frontignan cell cultures. Purification of the enzyme was achieved successively by ammonium sulfate precipitation and chromatography on DEAE-Sephacel, hydroxylapatite, Mono Q, Phenyl Superose, Superose 12, and preparative nondenaturing polyacrylamide gels. The enzyme formed only geranyl diphosphate as a product. In all cases, neither neryl diphosphate, the cis isomer, nor farnesyl diphosphate was detected. The enzyme showed a native molecular mass of 68 [plus or minus] 5 kD as determined …
Microbial inoculation for improving the growth and health of micropropagated strawberry
2004
Multimicrobial inoculation has been proposed as a way of protecting plants against environmental stress and increasing the sustainability of plant production. To study these possibilities in a micropropagation system, microplants of strawberry, Fragaria × ananssa, were inoculated or left uninoculated with five microorganisms ( Glomus mosseae BEG29, Bacillus subtilis M3, Trichoderma harzianumDB11, Pseudomonas fluorescensC7r12 and Gliocladium catenulatumGliomix ® ), used either singly or in dual mixtures in the presence or absence of the strawberry diseases crown rot ( Phytophthora cactorum) and red stele (P. fragariae). Finnish light Sphagnum peat was used as the growth substrate in the expe…
Elicitins trap and transfer sterols from micelles, liposomes and plant plasma membranes
1999
Using elicitins, proteins secreted by some phytopathogenic Oomycetes (Phytophthora) known to be able to transfer sterols between phospholipid vesicles, the transfer of sterols between micelles, liposomes and biological membranes was studied. Firstly, a simple fluorometric method to screen the sterol-carrier capacity of proteins, avoiding the preparation of sterolcontaining phospholipidic vesicles, is proposed. The transfer of sterols between DHE micelles (donor) and stigmasterol or cholesterol micelles (acceptor) was directly measured, as the increase in DHE fluorescence signal. The results obtained with this rapid and easy method lead to the same conclusions as those previously reported, u…
Current view of nitric oxide-responsive genes in plants
2009
International audience; Significant efforts have been directed towards the identification of genes differentially regulated through nitric oxide (NO)-dependent processes. These efforts comprise the use of medium- and large-scale transcriptomic analyses including microarray and cDNA-amplification fragment length polymorphism (AFLP) approaches. Numerous putative NO-responsive genes have been identified in plant tissues and cell suspensions with transcript levels altered by artificially released NO, or endogenously produced. Comparative analysis of the data from such transcriptomic analyses in Arabidopsis reveals that a significant part of these genes encode proteins related to plant adaptive …
Removal of 2-butoxyethanol gaseous emissions by biotrickling filtration packed with polyurethane foam
2016
The removal of 2-butoxyethanol from gaseous emissions was studied using two biotrickling filters (BTF1 and BTF2) packed with polyurethane foam. Two different inoculum sources were used: a pure culture of Pseudomonas sp. BOE200 (BTF1) and activated sludge from a municipal wastewater treatment plant (BTF2). The bioreactors were operated at inlet loads (ILs) of 130 and 195 g m(-3) hour(-1) and at an empty bed residence time (EBRT) of 12.5s. Under an IL of ∼130 g m(-3) hour(-1), BTF1 presented higher elimination capacities (ECs) than BTF2, with average values of 106±7 and 68±8 g m(-3) hour(-1), respectively. However, differences in ECs between BTFs were decreased by reducing the irrigation inte…
Short-term response of the slow growing seagrass Posidonia oceanica to simulated anchor impact
2007
Experimental evaluations about the impact of anchors of small vessels have previously shown that each anchoring can on average damage up to six shoots of Posidonia oceanica, removing small amount of biomass and, at the same time, interrupting continuity among shoots. The aim of the paper was to investigate the response of P. oceanica to different damage intensity at two levels of substrata compactness. Three treatments were considered: control (no damage); low damage (simulated anchor damage by three strokes of a hoe); and high damage (six strokes). Disturbance was higher where the substratum was highly penetrable and after one year significant variation was observed among treatments for bo…
Obtaining antioxidants and natural preservatives from food by-products through fermentation: A review
2021
Industrial food waste has potential for generating income from high-added-value compounds through fermentation. Solid-state fermentation is promising to obtain a high yield of bioactive compounds while requiring less water for the microorganism’s growth. A number of scientific studies evinced an increase in flavonoids or phenolics from fruit or vegetable waste and bioactive peptides from cereal processing residues and whey, a major waste of the dairy industry. Livestock, fish, or shellfish processing by-products (skin, viscera, fish scales, seabass colon, shrimp waste) also has the possibility of generating antioxidant peptides, hydrolysates, or compounds through fermentation. These bioacti…
Acclimatised rumen culture for raw microalgae conversion into biogas: Linking microbial community structure and operational parameters in anaerobic m…
2019
[EN] Ruminal fluid was inoculated in an Anaerobic Membrane Reactor (AnMBR) to produce biogas from raw Scenedesmus. This work explores the microbial ecology of the system during stable operation at different solids retention times (SRT). The 16S rRNA amplicon analysis revealed that the acclimatised community was mainly composed of Anaerolineaceae, Spirochaetaceae, Lentimicrobiaceae and Cloacimonetes fermentative and hydrolytic members. During the highest biodegradability achieved in the AnMBR (62%) the dominant microorganisms were Fervidobacterium and Methanosaeta. Different microbial community clusters were observed at different SRT conditions. Interestingly, syntrophic bacteria Gelria and …
Soil health through soil disease suppression: Which strategy from descriptors to indicators?
2007
International audience; Soil is a component of primary importance in crop production, even if it is often neglected, or only regarded as a physical support for the growth of plants. However, with the increasing societal concerns for the sustainability of agriculture, soil must be considered as a living system. Its quality results from the multiple interactions among physicochemical and biological components, notably the microbial communities, primordial for soil function. Crops are threatened by soil-borne diseases. These are often difficult to control, because of the “hidden” status of the pathogens and also because of the absence, noxiousness or lack of efficacy of chemical treatments. In…