Search results for "Acu"
showing 10 items of 6090 documents
Occlusion-derived baculovirus: interaction with human cells and evaluation of the envelope protein P74 as a surface display platform.
2008
To develop complementary baculovirus-based tools for gene delivery and display technologies, the interaction of occlusion-derived baculovirus (ODV) with human cells, and the functionality of the P74 ODV envelope protein for display of the IgG-binding Z domains (ZZP74) were evaluated. The cellular binding of ODV was concentration-dependent and saturable. Only minority of the bound virions were internalized at both 37 and 4 degrees C, suggesting usage of direct membrane fusion as the entry mode. The intracellular transport of ODV was confined in vesicular structures peripheral to the plasma membrane, impeding subsequent nuclear entry and transgene expression. Transduction of ODV was not rescu…
Baculovirus display strategies: Emerging tools for eukaryotic libraries and gene delivery
2004
Recombinant baculoviruses have been extensively used as vectors for abundant expression of a large variety of foreign proteins in insect cell cultures. The appeal of the system lies essentially in easy cloning techniques and virus propagation combined with the eukaryotic post-translational modification machinery of the insect cell. Recently, a novel molecular biology tool was established by the development of baculovirus surface display, using different strategies for presentation of foreign peptides and proteins on the surface of budded virions. This eukaryotic display system enables presentation of large complex proteins on the surface of baculovirus particles and has thereby become a ver…
Biochemical and structural analysis of the NS5B RNA-dependent RNA polymerase of the hepatitis C virus.
2000
Hepatitis C virus (HCV), the major causative agent of chronic and sporadic non-A, non-B hepatitis worldwide, is a distinct member of the Flaviviridae virus family. These viruses have in common a plus-strand RNA genome that is replicated in the cytoplasm of the infected cell via minus-strand RNA intermediates. Owing to the lack of reliable cell culture systems and convenient animal models for HCV, the mechanisms governing RNA replication are not known. As a first step towards the development of appropriate in vitro systems, we expressed the NS5B RNA-dependent RNA polymerase (RdRp) in insect cells, purified the protein to near homogeneity and studied its biochemical properties. It is a primer…
Immune responses during COVID-19 infection
2020
International audience; Over the past 16 years, three coronaviruses (CoVs), severe acute respiratory syndrome CoV (SARS-CoV) in 2002, Middle East respiratory syndrome CoV (MERS-CoV) in 2012 and 2015, and SARS-CoV-2 in 2020, have been causing severe and fatal human epidemics. The unpredictability of coronavirus disease-19 (COVID-19) poses a major burden on health care and economic systems across the world. This is caused by the paucity of in-depth knowledge of the risk factors for severe COVID-19, insufficient diagnostic tools for the detection of SARS-CoV-2, as well as the absence of specific and effective drug treatments. While protective humoral and cellular immune responses are usually m…
Molecular Basis of SARS-CoV-2 Nsp1-Induced Immune Translational Shutdown as Revealed by All-Atom Simulations.
2021
The severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) pandemic represents the most severe global health crisis in modern human history. One of the major SARS-CoV-2 virulence factors is nonstructural protein 1 (Nsp1), which, outcompeting with the binding of host mRNA to the human ribosome, triggers a translation shutdown of the host immune system. Here, microsecond-long all-atom simulations of the C-terminal portion of the SARS-CoV-2/SARS-CoV Nsp1 in complex with the 40S ribosome disclose that SARS-CoV-2 Nsp1 has evolved from its SARS-CoV ortholog to more effectively hijack the ribosome by undergoing a critical switch of Q/E158 and E/Q159 residues that perfects Nsp1's interactions…
Tumor targeting of baculovirus displaying a lymphatic homing peptide.
2008
Background Tumor-associated cells and vasculature express attractive molecular markers for site-specific vector targeting. To attain tumor-selective tropism, we recently developed a baculovirus vector displaying the lymphatic homing peptide LyP-1, originally identified by ex vivo/in vivo screening of phage display libraries, on the viral envelope by fusion to the transmembrane anchor of vesicular stomatitis virus G-protein. Methods In the present study, we explored the specificity and kinetics of viral binding and internalization as well as in vivo tumor homing of the LyP-1 displaying virus to elucidate the applicability of baculovirus for targeted therapies. Results We demonstrated that th…
Virskorekcijas pielietojamība tuvredzības progresijas novērtēšanā ortokeratoloģijas pacientiem
2022
Darbs ir uzrakstīts angļu valodā uz 93 lapaspusēm. Tas satur 49 attēlus, 24 tabulas un 95 atsauces uz literatūras avotiem. Šī darba mērķis ir pārbaudīt, vai virskorekcija ir derīga metode tuvredzības progresēšanas novērtēšanai ortokeratoloģijā. Hipotēze ir atrast lielākas izmaiņas gados jaunākiem pacientiem nekā pieaugušajiem, kā tas ir norādīts literatūrā. Pētījumā iekļauti 192 Eiropeīdās rases subjekti vecumā no 6 līdz 56 gadiem (vidēji 19,61±10,30). Izmantojot retrospektīvu analīzi, no objektīva BOZR kontaktlēcas parametra tiek iegūta virskorekcija (ORX). Jaunākiem cilvēkiem ir lielākas ORX vērtības, bet pieaugušajiem nav atšķirības (p<0,05). Vidēji tuvredzība progresēja par –0,18±0,44 D…
Redzes asuma treniņu efektivitāte
2018
Bakalaura darbs ir uzrakstīts angļu valodā uz 27. lappasspusēm, satur 12 attēlus, 1. tabulu un tajā ir atsauksmes uz 21. literatūras avotu. Darba mērķis ir novērtēt redzes asumu uzlabojošo treniņu efektivitāti. Viens no iemesliem pazeminātai redzei var būt palielināta tuvuma slodze, kā arī pazeminātas spējas veikt dažādus vizuālos uzdevumus. Trenējot šīs spējas, redzes sistēma labāk pielāgojas palielinātai tuvuma slodzei. Pētījumā piedalījās 24 bērni, kuriem tika doti dažādi redzes treniņi 3 mēnešu periodā. Redzes asums treniņu beigās būtiski uzlabojās (p<0.0001), salīdzinot ar treniņu sākumu. Vidējais uzlabojums bija 0,19 logMAR vienības. Dalībniekiem ar miopiju ametropijas lielums treniņu…
Stimula izvietojuma uz volumetriskā ekrāna ietekme uz acu kustībām
2021
Bakalaura darbs ir uzrakstīts latviešu valodā uz 37 lappusēm. Tas satur 19 attēlus, 1 tabulu un 56 atsauces uz literatūras avotiem. Darba mērķis ir noskaidrot, kā acu kustības ietekmē stimula izvietojums uz volumetriskā ekrāna. Visi dalībnieki veica vizuālās meklēšanas uzdevumu uz volumetriskā daudzplakņu ekrāna, norādot, kurš no četriem apļiem atradās tuvāk. Stimuliem tika mainīta to ekscentritāte – 2, 4, 6 un 8 grādi, kā arī tika iekļauti stimuli ar un bez mērķa elementa. Rezultāti parādīja, ka vizuālās meklēšanas uzdevuma izpildīšanas pareizība ir augstāka, ja tiek demonstrēts mērķa elements un stimuli atrodas tuvāk viens otram. Skata virziens biežāk tiek virzīts uz mērķa elementa atraša…
Traces of the past: an interview study with Finnish war children who did not return to Finland after the Second World War
2017
ABSTRACTThis paper is an in-depth qualitative study based on interviews with 10 Finnish children who were evacuated to Sweden during Second World War and who did not return to Finland after the war. The interviewees were asked to tell about their lives. Nine of them were between 2 and 5 years and one was 7 years old at the time of evacuation. The aim was to study how their childhood experiences were reflected in adult memories, how they remembered or did not remember. This paper focuses on the consequences of not knowing about one’s early life and also on whether it is possible to observe signs of the Finnish mother. She did not appear explicitly but could be sensed in the tendency of the i…