Search results for "Adaptation"
showing 5 items of 1775 documents
Enhancement of hepatitis C virus RNA replication by cell culture-adaptive mutations.
2001
ABSTRACT Studies of the Hepatitis C virus (HCV) replication cycle have been made possible with the development of subgenomic selectable RNAs that replicate autonomously in cultured cells. In these replicons the region encoding the HCV structural proteins was replaced by the neomycin phosphotransferase gene, allowing the selection of transfected cells that support high-level replication of these RNAs. Subsequent analyses revealed that, within selected cells, HCV RNAs had acquired adaptive mutations that increased the efficiency of colony formation by an unknown mechanism. Using a panel of replicons that differed in their degrees of cell culture adaptation, in this study we show that adaptive…
L’amica geniale in Inghilterra: il caso dell’adattamento radio di Timberlake Wertenbaker
2019
This paper takes into consideration Timberlake Wertenbaker's adaptation of Ferrante's My Brilliant Friend (2016) for the radio. In particular, the focus of the paper is on the first of the four radioplays and on the dramatic strategies used and adaptation choices made by Wertenbaker. We shall focus on the use of two main adult voices (Lena and Lila), the use of the voice over, and the representation of time. The chapter also considers the image of "neapolitanity" offered in the radioplay, the reception with the British audience with respect to the meridionist idea of Southern Italy as poor and violent. I aim to show how, with some exceptions, Wertenbaker somehow manages to push beyond the c…
Impact of Energy Availability, Health and Sex on Hemoglobin Mass Responses Following Live-High–Train-High Altitude Training in Elite Female and Male …
2018
Purpose: The authors investigated the effects of sex, energy availability (EA), and health status on the change in hemoglobin mass (ΔHbmass) in elite endurance athletes over ∼3–4 wk of live-high–train-high altitude training in Flagstaff, AZ (2135 m; n = 27 women; n = 21 men; 27% 2016 Olympians). Methods: Precamp and postcamp Hbmass (optimized carbon monoxide rebreathing method) and iron status were measured, EA was estimated via food and training logs, and a Low Energy Availability in Females Questionnaire (LEAFQ) and a general injury/illness questionnaire were completed. Hypoxic exposure (h) was calculated with low (600 h) groupings. Results: Absolute and relative percentage ΔHbmass was si…
Modeling multipartite virus evolution: the genome formula facilitates rapid adaptation to heterogeneous environments
2020
Multipartite viruses have two or more genome segments, and package different segments into different particle types. Although multipartition is thought to have a cost for virus transmission, its benefits are not clear. Recent experimental work has shown that the equilibrium frequency of viral genome segments, the setpoint genome formula (SGF), can be unbalanced and host-species dependent. These observations have reinvigorated the hypothesis that changes in genome-segment frequencies can lead to changes in virus-gene expression that might be adaptive. Here we explore this hypothesis by developing models of bipartite virus infection, leading to a threefold contribution. First, we show that th…