Search results for "Allozyme"
showing 10 items of 21 documents
Genetic Variability of Mediterranean Sea red mullet Mullus barbatus (Pisces, Mullidae)
1999
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Artificial environmental conditions can affect allozyme genetic structure of the marine gastropod Patella caerulea
2001
Samples of the Mediterranean limpet Patella caerulea collected from 10 sites were examined for genetic population structure. Six of the 20 identified enzymatic loci were polymorphic. The AAT* locus was polymorphic only in two samples from an artificial environment (TI2 and PE). The proportion of polymorphic loci ranged from 0.20 to 0.30, and the observed and expected mean heterozygosity varied between 0.098 and 0.076 and between 0.109 and 0.086, respectively. Mean Fis values were significantly positive in AAT*, ESTD*, PEPC-2*, and PEPD*, showing heterozygosity deficiency. In all, mean Fst value of 0.007 indicated high genetic homogeneity between the samples analyzed, whereas single-locus Fs…
Genetic and morphometric variations of Mediterranean hake,Merluccius merluccius,in the Strait of Sicily (central Mediterranean): Implications for sto…
2004
Allozyme, morphometric and growth analyses were applied to samples collected in the Strait of Sicily (central Mediterranean Sea) to test the hypothesis of the existence of a unique hake stock (Merluccius merluccius) in the study area. The level of genetic variation was detected from five polymorphic loci (ADH*, PGI-1; PGI-2*, PGM', SOD-1*). The average observed heterozygosity amounted to 0.421, while the average expected was 0.353. Weir & Cockerham statistics showed no heterogeneity, except for the single PGP locus (9 = 0.011; P 0.05). Morphometric analyses revealed some differentiation. Females showed different relationships in six out of eight morphometric indices with total length, while…
Does genetic population structure of Ambrosina bassii L. (Araceae, Ambrosineae) attest a post-Messinian land-bridge between Sicily and Africa?
2012
Abstract Aim of the present work is the analysis (through the study of enzyme polymorphism) of Sicilian and African (Tunisian) populations of Ambrosina bassii , a small perennial endemic to the Central-Western Mediterranean basin, in order to verify if the complex geological history of this part of the Mediterranean area left its mark in the present-day genetic structure of this taxon. Starch gel allozyme electrophoresis of seven putative loci of A. bassii was employed to estimate genetic diversity, genetic structure and gene flow. Populations from Sicily, Tunisia and Sardinia (as outgroup) were sampled. Results show that Sicily populations have 4 private alleles, Sardinia 3, Tunisia just o…
Effects of soil metal contamination on genetic diversity of the earthworm Dendrobaena octaedra (Savigny) in three metal contaminated areas in Finland
2007
Are there any distinct genetic sub-populations of sand smelt, Atherina boyeri (Teleostei: Atherinidae) along Italian coasts? Evidence from allozyme a…
2007
Two hundred and ninety-nine specimens of Atherina boyeri, collected from five Mediterranean sites (three from the open sea (Chioggia, Catania and Gaeta) and two from river mouths (the Birgi and S. Bartolomeo rivers) were analyzed in order to confirm previous genetic studies. Eigtheen loci were scored from 12 enzymatic systems, 3 of which were polymorphic: EST*, PGM* and PGI*. Mean Fst value (0.300, p< 0.05) indicated a high genetic heterogeneity. Pairwise Fst and genetic distance revealed that samples from the river mouths (Birgi and S. Bartolomeo) were grouped separately from the open sea samples (Catania, Chioggia and Gaeta). These results are in agreement with the most recent classificat…
Biochemical genetic differentiation between Pomatoschistus marmoratus and P. tortonesei
1999
Several diagnostic genetic markers were identified in Pomatoschistus marmoratus and P. tortonesei using polyacrylamide gel electrophoresis (PAGE) of allozymes. Twenty-one loci were resolved, including the electrophoretic pattern of muscle proteins. The MDH*, PGM1,2*, EST-1,2*, FUM* and PGI-2* loci exhibited different alleles which were fixed for the two species being analysed. Genetic distance, as calculated by Nei's index, showed a value of 0.413. Environmental hypersalinity, could have influenced the geographical distribution of P. tortonesei.
Genetic diversity and local population structure in Ambrosina bassii (Araceae, Ambrosineae), a Mediterranean relict species
2009
The effects of habitat fragmentation on the genetic structure of Ambrosina bassii are analyzed. The species, whose reproductive biology is mostly unknown, is the only representative of its genus and tribe and it is endemic to the central Mediterranean area. The selected study area was the island of Sicily, in which wild populations show a wide morphological variability and ecological amplitude. Patterns of within- and among-population genetic diversity in eleven Sicilian populations, occurring in six disjunct areas, were examined by means of allozyme electrophoresis. High levels of genetic diversity were found as shown by the mean expected heterozygosity (He ¼ 0.263), the percentage of poly…
Biochemical taxonomic differentiation between Mullus barbatus and mullus surmuletus (Pisces, mullidae)
1991
Abstract 1. 1. The electrophoretic data from seven enzymatic systems, codifying for 20 loci, and the patterns of general proteins from some tissues of Mullus barbatus and Mullus surmuletus were examined. 2. 2. The value of the genetic distance index as per Nei is 0.068, this criterium showing a high similarity between the two species. 3. 3. Species-specific patterns were found for the SOD enzyme and general proteins of the muscle.
Biochemical genetic differentiation between Pomatoschistus marmoratus and P. tortonesei (Pisces, Gobiidae).
1999
Several diagnostic genetic markers were identified in Pomatoschistus marmoratus and P. tortonesei using polyacrylamide gel electrophoresis (PAGE) of allozymes. Twenty-one loci were resolved, including the electrophoretic pattern of muscle proteins. The MDH*, PGM- 1,2*, EST-1,2*, FUM* and PGI-2* loci exhibited different alleles which were fixed for the two species being analysed. Genetic distance, as calculated by Nei’s index, showed a value of 0·413. Environmental hypersalinity, could have influenced the geographical distribution of P. tortonesei.