Search results for "Ande"

Villaggio (G. van Coninxloo),in G. C. SCIOLLA (a cura di), Cielo terra e acque: il paesaggio nella pittura fiamminga e olandese tra Cinquecento e Sei…

2006

Villaggio (G. van Coninxloo),in G. C. SCIOLLA (a cura di), Cielo terra e acque: il paesaggio nella pittura fiamminga e olandese tra Cinquecento e Seicento, Torino, Elede, 2006 p. 35-133

Medical masks and Respirators for the Protection of Healthcare Workers from SARS-CoV-2 and other viruses

2020

The use of medical masks and respirators as personal protective equipment is pivotal to reducing the level of biological hazard to which healthcare workers are exposed during the outbreak of highly diffusible pathogens, such as the recent novel coronavirus SARS-CoV-2. Unfortunately, during this pandemic, supplies are rapidly running out worldwide, with potential consequences for the rate of occupational infections. Also, knowledge about specific characteristics of respirators is of utmost importance to select the proper type according to the clinical setting. A wide variety of literature is available on the topic, but mostly based on Influenza viruses infection models. Clinical evidence on …

Design of a Virtual Learning Environment for Students with Special Needs

2006

The European Social Fund-supported Portland Partnership project developed a computer-based virtual learning environment (VLE) to benefit students with cognitive and physical disabilities. This system provided students with access to a suite of software programs to teach them basic/essential skills needed for everyday life and to use information and communications technology (ICT). The VLE can be customized to meet individual students’ needs by selecting an input device, adjusting its setting, or choosing a symbol set to support on-screen text. The learning programs within the VLE required careful design to make them stimulating and beneficial to students who have diverse health conditions a…

Management of pandemic influenza A (H1N1)2009 in the major University Hospital of Sicily

2011

Translocation of the nuclear autoantigen La to the cell surface of herpes simplex virus type 1 infected cells.

1992

Recently we developed a procedure to translocalize one of the extractable nuclear antigens (ENAs), the La protein, to the cell surface of CV-1 cells. Here we report that herpes simplex virus type 1 infection can also induce a translocation of the autoantigen to the cell surface. On the cell surface we detected La protein assembled with large protrusions. Within these protrusions La protein colocalized with virus particles. These protrusions are known to be released from the cell after virus infections. Such complexes consisting of self and virus could provide helper determinants for an anti-self response, and therefore be important in generation of autoimmunity.

The human autoantigen La/SS-B accelerates herpes simplex virus type 1 replication in transfected mouse 3T3 cells.

1998

SUMMARY Permanently transfected mouse cell lines which expressed different levels of the human autoantigen La/SS-B were infected with different strains of herpes simplex virus type 1, including the strains ANG, HSZP, 17syn+ and HFEM. During infection the localization of the human La protein was followed using an anti-La MoAb, which recognized only the human La protein but did not cross-react with either the endogenous mouse La protein or any viral encoded protein. After infection La protein was transported from the nucleus to the cytoplasm. The time course of translocation was dependent on the amount of human La protein expressed in the respective cell line. Moreover, acceleration of viral …

SARS-CoV-2 envelope protein topology in eukaryotic membranes

2020

Coronavirus E protein is a small membrane protein found in the virus envelope. Different coronavirus E proteins share striking biochemical and functional similarities, but sequence conservation is limited. In this report, we studied the E protein topology from the new SARS-CoV-2 virus both in microsomal membranes and in mammalian cells. Experimental data reveal that E protein is a single-spanning membrane protein with the N-terminus being translocated across the membrane, while the C-terminus is exposed to the cytoplasmic side (Nt lum /Ct cyt ). The defined membrane protein topology of SARS-CoV-2 E protein may provide a useful framework to understand its interaction with other viral and ho…

Characterization of the autoantigen La (SS-B) as a dsRNA unwinding enzyme

1997

During the analysis of the La (SS-B) autoantigen for catalytic activities an ATP-dependent double-stranded RNA unwinding activity was detected. Both native and recombinant La proteins from different species displayed this activity, which could be inhibited by monospecific anti-La antibodies. La protein was able to melt dsRNA substrates with either two 3'-overhangs or a single 3'- and a 5'-overhang. Double-stranded RNAs with two 5'-overhangs were not unwound, indicating that at least one 3'-overhang is required for unwinding. Sequence elements of the La protein that might be involved in dsRNA unwinding, such as an evolutionarily conserved putative ATP-binding motif and an element that is hom…

Turystyczny przewodnik po Piekle Mikołaja Reja, czyli o międzyplanetarnych podróżach duszy ludzkiej

2020

The article deals with the complex issue of presenting the underworld in the Reja's Wizerunk. The author draws attention to the presence in the output of the old Polish poet of many visionary elements borrowed from the most varied traditions and religious systems. The popular pattern of searching for spiritual perfection, fixed in the archetype of the topographic movement of the hero (Latin: Homo viator) turns out to be particularly visible. It turns out that at the very foundations of this imaginative pattern there is an ancient doctrine or interplanetary wandering of the soul. Most of the work was focused on the description of XI songs of Reja’s WizerunV, where a description of hell appea…

Study of vocalists' use of emotions in performance preparation

2015

Musicians and educators understand the importance of expressivity in vocal performance. Despite the acceptance of expressivity as a crucial element in musical performance, educators have reported addressing it only cursorily or not addressing it at all when preparing for a performance. For these reasons, this research has focused on techniques used by trained musicians when preparing a performance, to be adapted and applied for use by teachers with their large vocal ensembles. Participants in this study were enrolled in vocal / operatic performance programs at universities in the United States, Ireland, Finland, and Hong Kong. Their progress was tracked throughout the semester by three ques…