Search results for "Anther culture"
showing 5 items of 15 documents
STUDY ON GAMETIC EMBRYOGENESIS VIA IN VITRO ANTHER AND ISOLATED MICROSPORE CULTURE IN FRUIT CROPS
2014
Fruit breeding is mainly based on both conventional (hybridization, mutation and selection) or biotechnological methods (somatic hybridization, genetic transformation and haploid production). The genetic improvement through the conventional methods is limited by many factors such as fruit trees long juvenile period, high heterozygosity, large size and sexual incompatibility. Haploids and doubled haploids, obtained through gametic embryogenesis have a potential use in fruit crops genetic improvement. The change of the microspores fate from the normal gametopytic pathway towards the sporophytic induction is affected by numerous factors. Genotype, medium composition and stress were considered …
Somatic embryogenesis through in vitro anther culture ofCitrus sinensisL. Osbeck ‘Moro’
2019
In many crops, anther culture is the most used method to induce gametic embryogenesis, aimed to regenerate homozygous plants. However, also somatic embryogenesis can be obtained by this method, when somatic tissue is involved in regeneration process. Many factors can affect this procedure, such as genotype, pre-treatments applied to floral buds, pollen developmental stage, donor plant states, culture media composition and growth culture conditions. Anthers of Citrus sinensis L. Osbeck, cv. Moro, were collected at the vacuolate stage, and after a chilling (4°C) pre-treatment of 7 days, were placed on the same medium, evaluating different temperature stresses applied after the placing in cult…
Anther culture in Citrus:a powerful tool to recover seedless triploids
2004
Study on anther culture in hazelnut (Corylus avellana L.) .
2013
Effect of polyamines on in vitro anther culture of Citrus clementina Hort. ex Tan
2006
The improvement of the induction rate in Citrus anther culture is important for taking practical advantage of the haploid potential in breeding. The influence of polyamines on anther culture of Citrus clementina, cv Nules, with particular attention to the free, soluble and insoluble-conjugated polyamine levels, has been investigated. Putrescine, spermidine and putrescine plus spermidine, were added to the standard induction medium. Before culture, spermidine was the most abundant among the free polyamines detected in anthers. The exogenous supply of either putrescine or spermidine, either independently or combined, effected greater uptake and accumulation of polyamines. The addition of 2 mM…