Search results for "Antlers"

showing 2 items of 2 documents

Polish is quantitatively different on quartzite flakes used on different worked materials.

2020

Metrology has been successfully used in the last decade to quantify use-wear on stone tools. Such techniques have been mostly applied to fine-grained rocks (chert), while studies on coarse-grained raw materials have been relatively infrequent. In this study, confocal microscopy was employed to investigate polished surfaces on a coarse-grained lithology, quartzite. Wear originating from contact with five different worked materials were classified in a data-driven approach using machine learning. Two different classifiers, a decision tree and a support-vector machine, were used to assign the different textures to a worked material based on a selected number of parameters (Mean density of furr…

Future studiesConfocal MicroscopyDecision AnalysisLithologyRaw MaterialsAntlersBone imagingPlant Science01 natural sciencesDiagnostic RadiologyMedicine and Health Sciences0601 history and archaeologyElectron MicroscopyAnimal AnatomyMaterialsMicroscopyMultidisciplinary060102 archaeologyPlant AnatomyRadiology and ImagingQRLight Microscopy06 humanities and the artsQuartzWoodBone ImagingProcess EngineeringPhysical SciencesMedicineEngineering and TechnologyScanning Electron MicroscopyAnatomyManagement EngineeringGeologyResearch Article010506 paleontologyImaging TechniquesScienceMaterials ScienceMineralogyIndustrial ProcessesResearch and Analysis MethodsDiagnostic MedicineIndustrial Engineering0105 earth and related environmental sciencesSurface TreatmentsDecision TreesBiology and Life SciencesManufacturing ProcessesSample size determinationZoologyPloS one
researchProduct

An improved high yield method to obtain microsatellite genotypes from red deer antlers up to 200 years old.

2013

Analysis of DNA from older samples, such as museum specimen, is a promising approach to studying genetics of populations and ecological processes across several generations. Here, we present a method for extracting high quality nuclear DNA for microsatellite analysis from antlers of red deer (Cervus elaphus). The genotyping of individuals was based on nine microsatellite loci. Because the amount of DNA found in antlers was high, we could reduce the amount of sample and chemicals used and shorten the decalcification time in comparison to other methods. Using these methods, we obtained genotypes from antlers up to 200 years old.

animal structuresGenotypeZoologyAntlersBiologyPolymerase Chain ReactionChemistry Techniques Analyticallaw.inventionlawGermanyGenotypeGeneticsAnimalsGenotypingEcology Evolution Behavior and SystematicsPolymerase chain reactionGeneticsDeerMuseumsDecalcification TechniqueNuclear DNAMicrosatellite AnalysisMicrosatelliteCervus elaphusBiotechnologyMicrosatellite RepeatsMolecular ecology resources
researchProduct