Search results for "Ap4A"

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In human and rat lung membranes [35s]GTPγS binding is a tool for pharmacological characterization of G protein-coupled devucleotide receptors

1999

The P2Y receptor family is activated by extracellular nucleotides such as ATP and UTP. P2Y receptors regulate physiological functions in numerous cell types. In lung, the P2Y2 receptor subtype plays a role in controlling Cl- and fluid transport. Besides ATP or UTP, also diadenosine tetraphosphate (Ap4A), a stable nucleotide, seems to be of physiological importance. In membrane preparations from human and rat lung we applied several diadenosine polyphosphates to investigate whether they act as agonists for G protein-coupled receptors. We assessed this by determining the stimulation of [35S]GTPgammaS binding. Stimulation of [35S]GTPgammaS binding to G proteins has already been successfully ap…

P2Y receptorG proteinGTPgammaSReceptors Cell SurfaceBiologySulfur RadioisotopesGeneral Biochemistry Genetics and Molecular BiologyRadioligand Assaychemistry.chemical_compoundSpecies SpecificityGTP-Binding ProteinsAnimalsHumansGeneral Pharmacology Toxicology and PharmaceuticsReceptorLungG protein-coupled receptorG protein-coupled receptor kinaseMembranesReceptors Purinergic P2General MedicineFluid transportRatschemistryBiochemistryGuanosine 5'-O-(3-Thiotriphosphate)Ap4ALife Sciences
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