Search results for "Apoptosi"

Anti tumor action of the natural compound parthenolide

2010

TRAIL acts synergistically with iron oxide nanocluster-mediated magneto- and photothermia

2019

International audience; Targeting TRAIL (Tumor necrosis factor (TNF)-Related Apoptosis-Inducing Ligand) receptors for cancer therapy remains challenging due to tumor cell resistance and poor preparations of TRAIL or its derivatives. Herein, to optimize its therapeutic use, TRAIL was grafted onto iron oxide nanoclusters (NCs) with the aim of increasing its pro-apoptotic potential through nanoparticle-mediated magnetic hyperthermia (MHT) or photothermia (PT). Methods: The nanovector, NC@TRAIL, was characterized in terms of size, grafting efficiency, and potential for MHT and PT. The therapeutic function was assessed on a TRAIL-resistant breast cancer cell line, MDA-MB-231, wild type (WT) or T…

Adrb3 adrenergic receptor is a key regulator of human myometrial apoptosis and inflammation during chorioamnionitis1

2008

The pathophysiology underlying preterm labor triggered by inflammatory conditions such as chorioamnionitis remains largely unclear. It has already been suggested that beta-3 adrenergic (ADRB3) agonists might be of interest in the pharmacological management of preterm labor. Although there is evidence implicating ADRB receptors in the control of inflammation, there are minimal data relating specifically to ADRB3. To explore the cellular consequences of chorioamnionitis and detect apoptosis, we first performed immunostaining and Western blot experiments on human myometrial samples obtained from women with confirmed chorioamnionitis. We then developed an in vitro model of chorioamnionitis by i…

Efecto terapéutico del compuesto BO-110 en un modelo animal de leiomioma

2023

Los miomas son los tumores benignos más frecuentes en las mujeres en edad reproductiva y pueden asociar numerosos síntomas como dolor, sangrado y problemas reproductivos. Sin embargo, actualmente no existe una terapia totalmente satisfactoria por lo que es de suma importancia la evaluación de nuevos tratamientos. En la búsqueda de opciones terapéuticas alternativas, hemos seleccionado el ácido poliinosina-policitidílico (pIC) conjugado con polietilenimina (PEI) [pICPEI], cuyo nombre comercial es BO-100. El compuesto pIC es un ARN sintético de doble cadena sin toxicidad asociada, que induce de manera selectiva la autofagia y apoptosis en un gran espectro de células tumorales. Presen…

Significado pronóstico de las lesiones histopatológicas y de la expresión inmunohistoquímica de marcadores de apoptosis, proliferación y angiogénesis…

2008

Objetivo. Establecer una relación estadística entre características clínicas, histopatológicas con el grado de regresión tumoral Dworak y con el pronóstico. También se pretende estudiar distintos marcadores inmunohistoquímicos con el ojetivo de observar los cambios que sufre la la lesión neoplásica antes y después del tratamiento neoadyuvante en lo que respecta a la angiogénesis, proliferación, apoptosis y diferenciación neuroendocrina. Diseño de experimento: Se trata de un estudio epidemiológico retrospectivo con una muestra de 59 casos de carcinomas rectales diagnosticados en estadios avanzados y sometidos a neoadyuvancia. El análisis de las distintas variables histopatológicas e inmunohi…

Apoptosis rate in human germinal cells: quality assay.

2009

Apoptosis is a process that eliminates superfluous or genetically compromised cells. Cell death is one of the most important aspects of organization of the developing, as alteration in timing, level, or pattern of cell death can lead to developmental anomalies. In our studies, we investigated apoptosis in follicular somatic and germ cells of patients with infertility problems. We found that cell death occur in human germinal vesicle, in some mature oocytes and in the oocytes that failed fertilization after ICSI (Intracytoplasmic Sperm Injection), this failure was strictly correlated with apoptosis. Still, after the Italian law number 40 of 2004, it was not available oocytes to research, and…

Aplidin® induces JNK-dependent apoptosis in human breast cancer cells via alteration of glutathione homeostasis, Rac1 GTPase activation, and MKP-1 ph…

2006

Aplidin® is an antitumor agent in phase II clinical trials that induces apoptosis through the sustained activation of Jun N-terminal kinase (JNK). We report that Aplidin® alters glutathione homeostasis increasing the ratio of oxidized to reduced forms (GSSG/GSH). Aplidin® generates reactive oxygen species and disrupts the mitochondrial membrane potential. Exogenous GSH inhibits these effects and also JNK activation and cell death. We found two mechanisms by which Aplidin® activates JNK: rapid activation of Rac1 small GTPase and downregulation of MKP-1 phosphatase. Rac1 activation was diminished by GSH and enhanced by L-buthionine (SR)-sulfoximine, which inhibits GSH synthesis. Downregulatio…

Biological and Proteomic Characterization of the Anti-Cancer Potency of Aqueous Extracts from Cell-Free Coelomic Fluid of Arbacia lixula Sea Urchin i…

2022

Echinoderms are an acknowledged source of bioactive compounds exerting various beneficial effects on human health. Here, we examined the potential in vitro anti-hepatocarcinoma effects of aqueous extracts of the cell-free coelomic fluid obtained from the sea urchin Arbacia lixula using the HepG2 cell line as a model system. This was accomplished by employing a combination of colorimetric, microscopic and flow cytometric assays to determine cell viability, cell cycle distribution, the possible onset of apoptosis, the accumulation rate of acidic vesicular organelles, mitochondrial polarization, cell redox state and cell locomotory ability. The obtained data show that exposed HepG2 cells under…

The Anti-Cancer Effect of

2019

Ethanolic extracts from Mangifera indica L. have been proved to possess anti-tumor properties in many cancer systems. However, although most effects have been demonstrated with fruit pulp extract, the underlying molecular mechanisms of mango peel are still unclear. This study was designed to explore the effects of mango peel extract (MPE) on colon cancer cell lines. MPE affected cell viability and inhibited the colony formation trend of tumor cells, while no effects were observed in human dermal fibroblasts used as a non-cancerous cell line model. These events were a consequence of the induction of apoptosis associated to reactive oxygen species (ROS) production, activation of players of th…

A novel microtubule de-stabilizing complementarity-determining region C36L1 peptide displays antitumor activity against melanoma in vitro and in vivo

2015

AbstractShort peptide sequences from complementarity-determining regions (CDRs) of different immunoglobulins may exert anti-infective, immunomodulatory and antitumor activities regardless of the specificity of the original monoclonal antibody (mAb). In this sense, they resemble early molecules of innate immunity. C36L1 was identified as a bioactive light-chain CDR1 peptide by screening 19 conserved CDR sequences targeting murine B16F10-Nex2 melanoma. The 17-amino acid peptide is readily taken up by melanoma cells and acts on microtubules causing depolymerization, stress of the endoplasmic reticulum and intrinsic apoptosis. At low concentrations, C36L1 inhibited migration, invasion and proli…