Search results for "Apoptosis"

Efecto terapéutico del compuesto BO-110 en un modelo animal de leiomioma

2023

Los miomas son los tumores benignos más frecuentes en las mujeres en edad reproductiva y pueden asociar numerosos síntomas como dolor, sangrado y problemas reproductivos. Sin embargo, actualmente no existe una terapia totalmente satisfactoria por lo que es de suma importancia la evaluación de nuevos tratamientos. En la búsqueda de opciones terapéuticas alternativas, hemos seleccionado el ácido poliinosina-policitidílico (pIC) conjugado con polietilenimina (PEI) [pICPEI], cuyo nombre comercial es BO-100. El compuesto pIC es un ARN sintético de doble cadena sin toxicidad asociada, que induce de manera selectiva la autofagia y apoptosis en un gran espectro de células tumorales. Presen…

Significado pronóstico de las lesiones histopatológicas y de la expresión inmunohistoquímica de marcadores de apoptosis, proliferación y angiogénesis…

2008

Objetivo. Establecer una relación estadística entre características clínicas, histopatológicas con el grado de regresión tumoral Dworak y con el pronóstico. También se pretende estudiar distintos marcadores inmunohistoquímicos con el ojetivo de observar los cambios que sufre la la lesión neoplásica antes y después del tratamiento neoadyuvante en lo que respecta a la angiogénesis, proliferación, apoptosis y diferenciación neuroendocrina. Diseño de experimento: Se trata de un estudio epidemiológico retrospectivo con una muestra de 59 casos de carcinomas rectales diagnosticados en estadios avanzados y sometidos a neoadyuvancia. El análisis de las distintas variables histopatológicas e inmunohi…

Aplidin® induces JNK-dependent apoptosis in human breast cancer cells via alteration of glutathione homeostasis, Rac1 GTPase activation, and MKP-1 ph…

2006

Aplidin® is an antitumor agent in phase II clinical trials that induces apoptosis through the sustained activation of Jun N-terminal kinase (JNK). We report that Aplidin® alters glutathione homeostasis increasing the ratio of oxidized to reduced forms (GSSG/GSH). Aplidin® generates reactive oxygen species and disrupts the mitochondrial membrane potential. Exogenous GSH inhibits these effects and also JNK activation and cell death. We found two mechanisms by which Aplidin® activates JNK: rapid activation of Rac1 small GTPase and downregulation of MKP-1 phosphatase. Rac1 activation was diminished by GSH and enhanced by L-buthionine (SR)-sulfoximine, which inhibits GSH synthesis. Downregulatio…

Biological and Proteomic Characterization of the Anti-Cancer Potency of Aqueous Extracts from Cell-Free Coelomic Fluid of Arbacia lixula Sea Urchin i…

2022

Echinoderms are an acknowledged source of bioactive compounds exerting various beneficial effects on human health. Here, we examined the potential in vitro anti-hepatocarcinoma effects of aqueous extracts of the cell-free coelomic fluid obtained from the sea urchin Arbacia lixula using the HepG2 cell line as a model system. This was accomplished by employing a combination of colorimetric, microscopic and flow cytometric assays to determine cell viability, cell cycle distribution, the possible onset of apoptosis, the accumulation rate of acidic vesicular organelles, mitochondrial polarization, cell redox state and cell locomotory ability. The obtained data show that exposed HepG2 cells under…

The Anti-Cancer Effect of

2019

Ethanolic extracts from Mangifera indica L. have been proved to possess anti-tumor properties in many cancer systems. However, although most effects have been demonstrated with fruit pulp extract, the underlying molecular mechanisms of mango peel are still unclear. This study was designed to explore the effects of mango peel extract (MPE) on colon cancer cell lines. MPE affected cell viability and inhibited the colony formation trend of tumor cells, while no effects were observed in human dermal fibroblasts used as a non-cancerous cell line model. These events were a consequence of the induction of apoptosis associated to reactive oxygen species (ROS) production, activation of players of th…

A novel microtubule de-stabilizing complementarity-determining region C36L1 peptide displays antitumor activity against melanoma in vitro and in vivo

2015

AbstractShort peptide sequences from complementarity-determining regions (CDRs) of different immunoglobulins may exert anti-infective, immunomodulatory and antitumor activities regardless of the specificity of the original monoclonal antibody (mAb). In this sense, they resemble early molecules of innate immunity. C36L1 was identified as a bioactive light-chain CDR1 peptide by screening 19 conserved CDR sequences targeting murine B16F10-Nex2 melanoma. The 17-amino acid peptide is readily taken up by melanoma cells and acts on microtubules causing depolymerization, stress of the endoplasmic reticulum and intrinsic apoptosis. At low concentrations, C36L1 inhibited migration, invasion and proli…

Ultrasound bioeffects in rats: quantification of cellular damage in the fetal liver after pulsed Doppler imaging

2011

Objective To determine whether pulsed Doppler examination of the ductus venosus in rat fetuses could damage exposed tissue. Methods On gestational day 18, the livers of a mean of approximately five fetuses per mother (n = 5.14, SD = 1.6), in a cohort of 35 pregnant female rats, were exposed individually to pulsed Doppler and these were considered the 'exposed group'. The remaining fetuses in each pregnant rat (n = 5.16, SD = 2.1) formed the 'control group'. We tested for 600, 300, 60, 20, 15, 10 and 3 s of exposure of the fetal ductus venosus and the damage was evaluated measuring a cell death index of apoptotic activity at 7 h post-exposure (n = 16). In addition, subgroups of mothers were …

Sea urchin embryos cadmium exposed as an experimental model system for studying the relationship between autophagy and apoptosis

2012

Strategies in experimental models for evaluating apoptosis.

2008

Bcl-xL as a Modulator of Senescence and Aging

2021

Many features of aging result from the incapacity of cells to adapt to stress conditions. When cells are overwhelmed by stress, they can undergo senescence to avoid unrestricted growth of damaged cells. Recent findings have proven that cellular senescence is more than that. A specific grade of senescence promotes embryo development, tissue remodeling and wound healing. However, constant stresses and a weakening immune system can lead to senescence chronicity with aging. The accumulation of senescent cells is directly related to tissue dysfunction and age-related pathologies. Centenarians, the most aged individuals, should accumulate senescent cells and suffer from their deleterious effects,…