Search results for "Applied Microbiology and Biotechnology"
showing 10 items of 1090 documents
Collateral sensitivity of natural products in drug-resistant cancer cells
2018
Cancer chemotherapy is frequently hampered by drug resistance. Concepts to combine anticancer drugs with different modes of action to avoid the development of resistance did not provide the expected success in the past, because tumors can be simultaneously non-responsive to many drugs (e.g. the multidrug resistance phenotype). However, tumors may be specifically hypersensitive to other drugs - a phenomenon also termed collateral sensitivity. This seems to be a general biological mechanism, since it also occurs in drug-resistant Escherichia coli and Saccharomyces cerevisiae. Here, we give a timely and comprehensive overview on hypersensitivity in resistant cancer cells towards natural produc…
Fatty acid hydroperoxide lyase of green bell pepper: cloning in Yarrowia lipolytica and biogenesis of volatile aldehydes
2004
Publication Inra prise en compte dans l'analyse bibliométrique des publications scientifiques mondiales sur les Fruits, les Légumes et la Pomme de terre. Période 2000-2012. http://prodinra.inra.fr/record/256699; International audience; Fatty acid hydroperoxide lyase (HPO lyase) is a cytochrome P450 acting on fatty acid hydroperoxides in many organisms. The expression of green bell pepper HPO lyase in the yeast Yarrowia lipolytica is described for the first time. HPO lyase activity from yeast extract and whole yeast cells is measured and aldehydes production from yeast extract and whole yeast cells is compared. 1200 U/L reaction medium were obtained after 96 h of culture on olive oil rich me…
Species disparity response to mutagenesis of marine yeasts for the potential production of biodiesel.
2019
Abstract Background Among the third-generation biodiesel feed stock, oleaginous marine yeasts are the least studied microorganisms for such purpose. Results Wild strains yeasts were isolated from various Tunisian marine sources including fish waste (Candida tenuis CtTun15, Debaryomyces hansenii DhTun2015, Trichosporon asahii TaTun15 and Yarrowia lipolytica YlTun15) and seawater (Rhodotorula mucilaginosa RmTun15). Following incubation with ethyl methanesulfonate (EMS: 75 mM) for various periods of time (T15, T30, T45, T60 min), the cell viability of these strains responded differentially according to yeast species. For instance, mutated CtTun15 did not survive after 30 min of EMS treatment; …
Use of solid state fermentation to produce Beauveria bassiana for the biological control of european corn borer
1993
The production process of a new bioinsecticide against european corn borer is described. The entomopathogenic fungus, Beauveria bassiana, is cultivated by Solid State Fermentation (SSF). The culture support chosen, clay microgranules, humidified with optimal nutritive solution, is incubated in optimal conditions during 48 hours, then dried for 5 days. The bioinsecticide can be directly used after harvesting, without formulation. This process is original for several reasons : - The granulometry (500 microm) and the bulk density (0.6) of the microgranules are compatible with the mechanical standard application of pesticides on corn. - The bioinsecticide could be produced in a pilot reactor of…
Influence of agronomic practices and pre-harvest conditions on the attachment and development of Listeria monocytogenes in vegetables
2019
Interest in fresh vegetables is on the increase due to their protective effects against several diseases. Listeria monocytogenes is a human pathogen easily found in vegetables. The purpose of this review article is to analyse the influence of the agricultural practices applied in pre-harvest, the environmental biotic and abiotic factors characterising the cultivation field, as well as the handling procedures at harvest that might greatly influence the presence and the levels of L. monocytogenes in fresh produce. This review article describes the routes of L. monocytogenes infections in relation to the agricultural practices commonly applied during vegetable cultivation. It also analyses the…
Colonization of flax roots and early physiological responses of flax cells inoculated with pathogenic and nonpathogenic strains of Fusarium oxysporum
2003
ABSTRACT Fusarium oxysporum includes nonpathogenic strains and pathogenic strains that can induce necrosis or tracheomycosis in plants. The objective of this study was to compare the abilities of a pathogenic strain (Foln3) and a nonpathogenic strain (Fo47) to colonize flax roots and to induce early physiological responses in flax cell culture suspensions. Both strains colonized the outer cortex of the root; however, plant defense reactions, i.e., the presence of wall appositions, osmiophilic material, and collapsed cells, were less frequent and less intense in a root colonized by Foln3 than by Fo47. Early physiological responses were measured in flax cell suspensions confronted with germin…
Effects of Bacillus thuringiensis δ-Endotoxins on the Pea Aphid ( Acyrthosiphon pisum )
2009
ABSTRACT Four Bacillus thuringiensis δ-endotoxins, Cry3A, Cry4Aa, Cry11Aa, and Cyt1Aa, were found to exhibit low to moderate toxicity on the pea aphid, Acyrthosiphon pisum , in terms both of mortality and growth rate. Cry1Ab was essentially nontoxic except at high rates. To demonstrate these effects, we had to use exhaustive buffer-based controls.
Deletion of GLX3 in Candida albicans affects temperature tolerance, biofilm formation and virulence.
2018
Candida albicans is a predominant cause of fungal infections in mucosal tissues as well as life-threatening bloodstream infections in immunocompromised patients. Within the human body, C. albicans is mostly embedded in biofilms, which provides increased resistance to antifungal drugs. The glyoxalase Glx3 is an abundant proteomic component of the biofilm extracellular matrix. Here, we document phenotypic studies of a glx3Δ null mutant concerning its role in biofilm formation, filamentation, antifungal drug resistance, cell wall integrity and virulence. First, consistent with its function as glyoxalase, the glx3 null mutant showed impaired growth on media containing glycerol as the carbon sou…
Different bacteria can enhance the micropropagation response of Cotoneaster lacteus (Rosaceae)
1998
C. MONIER, E. BOSSIS, C. CHABANET AND R. SAMSON. 1998. Cotoneaster species are widely used ornamental shrubs, and research is under way to select genotypes which are more resistant to fire blight by somaclonal variation. During the establishment of micropropagation, one genotype, Cotoneaster lacteus, showed the development of endogenous bacterial contaminants. Two strains were isolated from in vitro explants: Bacillus pumilus and Alcaligenes faecalis. These two strains, and some Pseudomonas fluorescens and Ps. putida, promoted in vitro multiplication and rooting of explants. In Pseudomonas strains, rooting enhancing capacity appeared to be related to indole-3-acetic acid (IAA) production, a…
Bioprospecting for brewers: Exploiting natural diversity for naturally diverse beers.
2019
The burgeoning interest in archaic, traditional, and novel beer styles has coincided with a growing appreciation of the role of yeasts in determining beer character as well as a better understanding of the ecology and biogeography of yeasts. Multiple studies in recent years have highlighted the potential of wild Saccharomyces and non‐Saccharomyces yeasts for production of beers with novel flavour profiles and other desirable properties. Yeasts isolated from spontaneously fermented beers as well as from other food systems (wine, bread, and kombucha) have shown promise for brewing application, and there is evidence that such cross‐system transfers have occurred naturally in the past. We revie…