Search results for "Arabidopsi"
showing 10 items of 241 documents
Identification of a Functional Homolog of the Yeast Copper Homeostasis Gene ATX1 from Arabidopsis1
1998
Abstract A cDNA clone encoding a homolog of the yeast (Saccharomyces cerevisiae) gene Anti-oxidant 1(ATX1) has been identified from Arabidopsis. This gene, referred to as CopperCHaperone(CCH), encodes a protein that is 36% identical to the amino acid sequence of ATX1 and has a 48-amino acid extension at the C-terminal end, which is absent from ATX1 homologs identified in animals. ATX1-deficient yeast (atx1) displayed a loss of high-affinity iron uptake. Expression of CCH in the atx1 strain restored high-affinity iron uptake, demonstrating thatCCH is a functional homolog of ATX1. When overexpressed in yeast lacking the superoxide dismutase geneSOD1, both ATX1 and CCHprotected the cell from t…
2004
Background Arabidopsis thaliana copper metallochaperone CCH is a functional homologue of yeast antioxidant ATX1, involved in cytosolic copper transport. In higher plants, CCH has to be transported to specialised cells through plasmodesmata, being the only metallochaperone reported to date that leaves the cell where it is synthesised. CCH has two different domains, the N-terminal domain conserved among other copper-metallochaperones and a C-terminal domain absent in all the identified non-plant metallochaperones. The aim of the present study was the biochemical and biophysical characterisation of the C-terminal domain of the copper metallochaperone CCH.
Profundización en la caracterización funcional de las isoformas glicolíticas de la gliceraldehído-3-fosfato deshidrogenasa de Arabidopsis
2015
La glicólisis es una ruta metabólica que se encuentra, al menos en parte, en todos los organismos vivos, y es una de las primeras grandes rutas cuyas enzimas fueron identificadas y caracterizadas a nivel bioquímico. En los últimos años, se ha demostrado que las enzimas que participan en la glicólisis están también implicadas en otros procesos como por ejemplo en la señalización celular. En esta tesis doctoral, ha sido el objeto de nuestro estudio las isoformas glicolíticas de la gliceraldehído-3-fosfato deshidrogenasa (GAPDH) fosforilativa de Arabidopsis thaliana. Las distintas isoformas de esta enzima participan en el ciclo de Calvin en los cloroplastos, y en la glicólisis en el citosol y …
Defective copper transport in the copt5 mutant affects cadmium tolerance.
2014
Cadmium toxicity interferes with essential metal homeostasis, which is a problem for both plant nutrition and the consumption of healthy food by humans. Copper uptake is performed by the members of the Arabidopsis high affinity copper transporter (COPT) family. One of the members, COPT5, is involved in copper recycling from the vacuole toward the cytosolic compartment. We show herein that copt5 mutants are more sensitive to cadmium stress than wild-type plants, as indicated by reduced growth. Exacerbated cadmium toxicity in copt5 mutants is due specifically to altered copper traffic through the COPT5 transporter. Three different processes which have been shown to affect cadmium tolerance ar…
Data from: Experimental evolution of an emerging plant virus in host genotypes that differ in their susceptibility to infection
2014
This study evaluates the extent to which genetic differences among host individuals from the same species conditions the evolution of a plant RNA virus. We performed a three-fold replicated evolution experiment in which Tobacco etch potyvirus isolate At17b (TEV-At17b), adapted to Arabidopsis thaliana ecotype Ler-0, was serially passaged in five genetically heterogeneous ecotypes of A. thaliana. After 15 passages we found that evolved viruses improved their fitness, showed higher infectivity and stronger virulence in their local host ecotypes. The genome of evolved lineages was sequenced and putative adaptive mutations identified. Host-driven convergent mutations have been identified. Eviden…
Deregulated Copper Transport Affects Arabidopsis Development Especially in the Absence of Environmental Cycles
2010
Abstract Copper is an essential cofactor for key processes in plants, but it exerts harmful effects when in excess. Previous work has shown that the Arabidopsis (Arabidopsis thaliana) COPT1 high-affinity copper transport protein participates in copper uptake through plant root tips. Here, we show that COPT1 protein localizes to the plasma membrane of Arabidopsis cells and the phenotypic effects of transgenic plants overexpressing either COPT1 or COPT3, the latter being another high-affinity copper transport protein family member. Both transgenic lines exhibit increased endogenous copper levels and are sensitive to the copper in the growth medium. Additional phenotypes include decreased hypo…
Characterization of Erwinia gerundensis A4, an Almond-Derived Plant Growth-Promoting Endophyte
2021
The rapidly increasing global population and anthropogenic climate change have created intense pressure on agricultural systems to produce increasingly more food under steadily challenging environmental conditions. Simultaneously, industrial agriculture is negatively affecting natural and agricultural ecosystems because of intensive irrigation and fertilization to fully utilize the potential of high-yielding cultivars. Growth-promoting microbes that increase stress tolerance and crop yield could be a useful tool for helping mitigate these problems. We investigated if commercially grown almonds might be a resource for plant colonizing bacteria with growth promotional traits that could be use…
Regulación espacio-temporal de la homeostasis del cobre en Arabidopsis thaliana
2013
El cobre y el hierro son nutrientes esenciales para los seres vivos aerobios y sus potenciales redox son utilizados en las reacciones de transferencia de electrones. Sin embargo, esta capacidad redox resulta tóxica cuando los metales de transición entran en contacto con intermediarios del metabolismo aerobio, conocidos como ROS (reactive oxygen species). Los organismos aerobios han desarrollado estrategias homeostáticas para mantener los niveles de los metales adecuados en la célula y distribuir, espacial y temporalmente, los procesos de transporte y tráfico intracelular de metales para minimizar el daño oxidativo al resto de procesos celulares. En este sentido, recientemente se ha demostra…
Putative sorting signals involved in PIN1 trafficking
2013
Las auxinas son hormonas vegetales involucradas en muchos procesos fisiológicos, tales como el crecimiento y diferenciación celular, la maduración de los frutos, la floración o los tropismos. Su transporte a través de la planta es polar, jugando un papel esencial en la regulación del crecimiento y el desarrollo y contribuyendo al mantenimiento de la polaridad global de la planta. La polaridad en el flujo de auxinas es debida, al menos en parte, a la distribución polar de los transportadores de salida de auxina de la familia PIN. En Arabidopsis thaliana se han identificado 8 transportadores PIN diferentes. Todos ellos presentan una estructura similar, con un dominio citosólico central que se…
Overexpression of SAMDC1 gene in Arabidopsis thaliana increases expression of defense-related genes as well as resistance to Pseudomonas syringae and…
2014
It has been previously described that elevation of endogenous spermine levels in Arabidopsis could be achieved by transgenic overexpression of S-Adenosylmethionine decarboxylase (SAMDC) or Spermine synthase (SPMS). In both cases, spermine accumulation had an impact on the plant transcriptome, with up-regulation of a set of genes enriched in functional categories involved in defense-related processes against both biotic and abiotic stresses. In this work, the response of SAMDC1-overexpressing plants against bacterial and oomycete pathogens has been tested. The expression of several pathogen defense-related genes was induced in these plants as well as in wild type plants exposed to an exogeno…