Search results for "Array"

Ad-Hoc Segmentation Pipeline for Microarray Image Analysis

2006

Microarray is a new class of biotechnologies able to help biologist researches to extrapolate new knowledge from biological experiments. Image Analysis is devoted to extrapolate, process and visualize image information. For this reason it has found application also in Microarray, where it is a crucial step of this technology (e.g. segmentation). In this paper we describe MISP (Microarray Image Segmentation Pipeline), a new segmentation pipeline for Microarray Image Analysis. The pipeline uses a recent segmentation algorithm based on statistical analysis coupled with K-Means algorithm. The Spot masks produced by MISP are used to determinate spots information and quality measures. A software …

Network Pharmacology of Ginseng (Part II): The Differential Effects of Red Ginseng and Ginsenoside Rg5 in Cancer and Heart Diseases as Determined by …

2021

Panax ginseng C.A.Mey. is an adaptogenic plant traditionally used to enhance mental and physical capacities in cases of weakness, exhaustion, tiredness, or loss of concentration, and during recovery. According to ancient records, red ginseng root preparations enhance longevity with long-term intake. Recent pharmacokinetic studies of ginsenosides in humans and our in vitro study in neuronal cells suggest that ginsenosides are effective when their levels in blood is low—at concentrations from 10−6 to 10−18 M. In the present study, we compared the effects of red ginseng root preparation HRG80TM(HRG) at concentrations from 0.01 to 10,000 ng/mL with effects of white ginseng (WG) and purified gin…

Optimal implementation of neural activation functions in programmable logic using fuzzy logic

2006

Abstract This work presents a methodology for implementing neural activation function in programmable logic using tools from fuzzy logic. This methodology will allow implementing these intrinsic non-linear functions using comparators and simple linear modellers, easily implemented in programmable logic. This work is particularized to the case of a hyperbolic tangent, the most common function in neural models, showing the excellent results yielded with the proposed approximation.

FRUIT QUALITY EVALUATION OF FOUR LOQUAT CULTIVARS GROWN IN SICILY

2011

Chemical (soluble solids content, titratable acidity, pH), morphological (weight, longitudinal and transversal diameter, thickness of the flesh, number of seeds, peel colour), and sensorial characteristics of four loquat cultivars (‘Algerie’, ‘Claudia’, ‘Nespolone di Trabia’ and ‘Sanfilippara’) from mature, organically-grown trees in Sicily, were analysed. The determinations were carried out by traditional instrumental techniques, by a panel test and by an electronic olfactory system. A panel of 10 trained judges was used to determine intensity of some attributes in the sensory profile of the different cultivars, while the electronic olfactory system was used to identify possible difference…

La tecnologia del DNA-microarray per l'identificazione di specie microbiche su superfici e nell'aerosol di ambienti confinati

2009

La tecnologia del DNA microarray è utilizzata nei nostri laboratori per lo sviluppo di piattaforme multi-diagnostiche finalizzate alla identificazione di microrganismi. Il sistema è applicabile a campioni biologici prelevati in ambienti confinati, dove funghi e batteri rappresentano una delle componenti principali. Questi microrganismi, sono in grado di svilupparsi, in stretta relazione con determinati parametri come: i valori di temperatura e umidità relativa, le caratteristiche chimico fisiche dei materiali, e delle superfici, la tipologia di aerosol di questi ambienti. L’instaurarsi di consorzi microbici complessi e stabili oltre ad accelerare il degrado superficiale dei materiali, può c…

Neurobeachin (NBEA) is downregulated in blood cells from a patient with autism spectrum disorders (ASD)

2007

Identification of pathways involved in aneuploidy onset and its tolerance using a DNA microarray approach

2013

Genomic instability is a hallmark of the majority of human tumors explaining the heterogeneity shown by tumor cells. This phenomenon is often associated with chromosomal instability (CIN) and aneuploidy, a condition in which tumor cells lose or gain chromosomes. Previously, we showed that posttranscriptional silencing by RNAi of pRb1, DNMT12 and MAD2 is associated with aneuploidy in cultured human cells reinforcing the idea that there are several roads leading to aneuploidy. In the attempt to understand if a common molecular signature exists underlying aneuploidy and its tolerance in tumor cells, we induced aneuploidy in human fibroblasts (IMR90) by depleting Rb, MAD2 and DNMT1 genes and an…

Identification of molecular markers involved in cellular response to aneuploidy in normal and tumor cells

2020

Dal trascrittoma all’interattoma di miRNA: identificazione sperimentale e bioinformatica delle interazioni funzionali miRNA:mRNA

2013

I miRNA, piccole molecole endogene di RNA non codificante, regolano l’espressione genica attraverso la degradazione dei messaggeri (mRNA) o l’inibizione della traduzione. I miRNA maturi interagiscono con le proteine del complesso RISC (RNA-induced silencing complex) tra cui le proteine Argonaute (Ago), capaci di legare direttamente i miRNA e di mediare la regolazione dell’espressione genica in seguito alla interazione del miRNA con il proprio mRNA target. Un singolo miRNA può legare diversi mRNA e ciascun mRNA può essere regolato da diversi miRNA. La maggior parte dei software di predizione oggi disponibili individuano i putativi target di singoli miRNA ignorando caratteristiche di tipo glo…

A MULTI-LAYER MODEL TO STUDY GENOME-SCALE POSITIONS OF NUCLEOSOMES

2007

The positioning of nucleosomes along chromatin has been implicated in the regulation of gene expression in eukaryotic cells, because packaging DNA into nucleosomes affects sequence accessibility. In this paper we propose a new model (called MLM) for the identification of nucleosomes and linker regions across DNA, consisting in a thresholding technique based on cut-set conditions. For this purpose we have defined a method to generate synthetic microarray data fully inspired from the approach that has been used by Yuan et al. Results have shown a good recognition rate on synthetic data, moreover, the $MLM$ shows a good agreement with the recently published method based on Hidden Markov Model …