Search results for "Array"
showing 10 items of 1264 documents
HIF-1α induces MXI1 by alternate promoter usage in human neuroblastoma cells
2009
Adaptation to low oxygen conditions is essential for maintaining homeostasis and viability in oxygen-consuming multi-cellular tissues, including solid tumors. Central in these processes are the hypoxia-inducible transcription factors, HIF-1 and HIF-2, controlling genes involved in e.g. glucose metabolism and neovascularization. Tumor hypoxia and HIF expression have also been associated with a dedifferentiated phenotype and increased aggressiveness. In this report we show that the MAX interactor-1 (MXI1) gene is directly regulated by HIF proteins in neuroblastoma and breast cancer cells. HIF-binding and transactivation were detected within MXI1 gene regulatory sequences in the vicinity of th…
Lungscape: Resected Non–Small-Cell Lung Cancer Outcome by Clinical and Pathological Parameters
2014
INTRODUCTION: The Lungscape project was designed to address the impact of clinical, pathological, and molecular characteristics on outcome in resected non-small- cell lung cancer (NSCLC).MATERIALS AND METHODS: A decentralized biobank with fully annotated tissue samples was established. Selection criteria for participating centers included sufficient number of cases, tissue microarray building capability, and documented ethical approval. Patient selection was based on availability of comprehensive clinical data, radical resection between 2003 and 2009 with adequate follow-up, and adequate quantity and quality of formalin-fixed tissue.RESULTS: Fifteen centers contributed 2449 cases. The 5-yea…
WIDAR: Bistatic WI-fi Detection And Ranging for off-the-shelf devices
2013
The huge spread of wireless networks and the success of location-aware applications require novel indoor po- sitioning mechanisms based on existing technologies such as IEEE 802.11. Taking inspiration from the RADAR, we propose WIDAR: a bistatic WI-fi Detection And Ranging system for off- the-shelf devices. WIDAR implementation is based on the USRP2 platform and is able to locate 802.11 stations while they operate in existing legacy networks. No substitution or repositioning of the Access Points is necessary. WIDAR works passively and does not expect any dedicated action from the target WiFi node. No airtime is wasted and the target cannot even detect that it is being ranged. Such features …
INFERRING GENE NETWORKS FROM MICROARRAY WITH GRAPHICAL MODELS
2013
ABSTRACT. Microarray technology allows to collect a large amount of genetic data, such as gene expression data. The activity of the genes are coordinate by a complex network that regulates their expressions controlling common functions, such as the formation of a transcriptional complex or the availability of a signalling pathway. Understanding this organization is crucial to explain normal cell physiology as well as to analyse complex pathological phenotypes. Graphical models are a class of statistical models that can be used to infer gene regulatory networks. In this paper, we examine a class of graphical models: the strongly decomposable graphical models for mixed variables. Among oth- e…
A Parallel Face Detection System Implemented on FPGA
2007
In this paper, we introduce a methodology for designing a system for face detection and its implementation on FPGA. The chosen face detection method is the well-known convolutional face finder (CFF) algorithm, which consists in a pipeline of convolutions and subsampling operations. Our goal is to define a parallel architecture able to process efficiently this algorithm. We present a dataflow based architecture algorithm adequation (AAA) methodology implemented using the SynDEx software, in order to find the best compromise between the processing power and functionality requirement of each processor element (PE), and the efficiency of algorithm parallelization. We describe a first implementa…
Extracorporeal shock wave-mediated changes in proliferation, differentiation, and gene expression of human osteoblasts.
2008
The goal of this study was to determine whether cell proliferation, differentiation, and gene expression of primary human osteoblasts (hOB) are influenced by shock wave application (SWA).Osteoblast cultures were isolated from cancellous bone fragments and treated with 500 impulses of energy flux densities of 0.06 mJ/mm, 0.18 mJ/mm, 0.36 mJ/mm, and 0.50 mJ/mm. Twenty-four hours and 96 hours after SWA cell proliferation, alkaline phosphatase activity, and mineralization were analyzed. The global gene expression profiling was determined 96 hours after SWA employing Affymetrix HG-U133A microarrays.After 24 hours, hOB showed a dose-dependent increase in cell proliferation from 68.7% (at 0.06 mJ/…
Genomic response of the rat brain to global ischemia and reperfusion
2008
To identify genes that are involved in ischemia response of the brain, we have evaluated changes of gene expression in rat cerebrum after 15 min complete global ischemia, followed by reperfusion for 1 h, 6 h or 24 h. The expression profiles of approximately 30,000 transcripts from three subjects in each group (including sham-operated controls) were monitored employing oligonucleotide microarrays. About 20,000 transcripts were detectable in rat brains. The levels of 576 transcripts (approximately 2.9%) were significantly altered in response to experimental ischemia. 419 transcripts were up- and 157 downregulated; 39 transcripts changed after 1 h reperfusion, 174 after 6 h and 462 after 24 h.…
Spatio-temporal dynamics of oscillatory network activity in the neonatal mouse cerebral cortex
2007
We used a 60-channel microelectrode array to study in thick (600-1000 microm) somatosensory cortical slices from postnatal day (P)0-P3 mice the spatio-temporal properties of early network oscillations. We recorded local non-propagating as well as large-scale propagating spontaneous oscillatory activity. Both types of activity patterns could never be observed in neocortical slices of conventional thickness (400 microm). Local non-propagating spontaneous oscillations with an average peak frequency of 15.6 Hz, duration of 1.7 s and maximal amplitude of 66.8 microV were highly synchronized in a network of approximately 200 microm in diameter. Spontaneous oscillations of lower frequency (10.4 Hz…
Synthesis of a Linear Assembly Consisting of a Central Ru(Phen)32+ Derivative and Two Peripheral Porphyrins
2002
A ruthenium(II) precursor complex containing a bis-phenanthroline ligand, leading to a controlled helical structure around the metal centre, and a third phenanthroline-type ligand has been prepared. The spatial arrangement of the system is such that two chemical groups are disposed trans to one another on an axis running through the Ru centre. By appropriate functional group transformation, these two groups can be attached to monosubstituted zinc(II) porphyrins (PZn) to afford a linear array consisting of a central ruthenium(II) complex and two peripheral PZn motifs. (© Wiley-VCH Verlag GmbH, 69451 Weinheim, Germany, 2002)
A new preprocessing and control board for the phase 2 electronics of AGATA experiment
2016
The electronics of AGATA HPGe segmented gamma ray detector faces a new challenge in the search of a bigger integration and cost reduction for the phase 2 of the experiment going beyond 45 crystals. This opportunity can be used to introduce a new architecture based on commercial standards while keeping backward compatibility with current electronics. In this sense, new FPGA devices and fast Ethernet links can be used to ease the preprocessing and control task and allowing for processor farms to distribute the processing load. At the same time, modularity should be a key feature of the design in the aim to make it upgradable in time and technology. This paper presents the design of a new prep…