Search results for "Arum"

Unnatural amino acids increase activity and specificity of synthetic substrates for human and malarial cathepsin C

2014

Mammalian cathepsin C is primarily responsible for the removal of N-terminal dipeptides and activation of several serine proteases in inflammatory or immune cells, while its malarial parasite ortholog dipeptidyl aminopeptidase 1 plays a crucial role in catabolizing the hemoglobin of its host erythrocyte. In this report, we describe the systematic substrate specificity analysis of three cathepsin C orthologs from Homo sapiens (human), Bos taurus (bovine) and Plasmodium falciparum (malaria parasite). Here, we present a new approach with a tailored fluorogenic substrate library designed and synthesized to probe the S1 and S2 pocket preferences of these enzymes with both natural and a broad ran…

A 79-year-old-man with SARS-CoV-2 pneumonia and unusual pulmonary co-infection

2022

Patients with severe COVID-19 are characterized by local and systemic inflammatory responses, which are responsible for severe acute respiratory failure. Systemic corticosteroids have been used to counteract the inflammatory cascade in severe respiratory patients. The beneficial effect of corticosteroids may be counteracted by adverse effects, including delayed viral clearance and increased risk of secondary infection. In this regard, we describe a case of a 79-year-old man admitted to the Emergency Department of the Palermo Hospital (Italy) because of respiratory failure and pneumonia caused by SARS-CoV-2 infection who developed Nocardia otitidiscaviarum infection. This case highlights the…

Infección pulmonar por Nocardia otitidiscaviarum en paciente inmunocompetente

2019

Aplicación de la topología molecular a la predicción de la actividad antimalárica de análogos de la 4-anilinoquinolina

2021

La malaria es una enfermedad parasitaria causada por protozoos del género Plasmodium y transmitida por vectores del género Anopheles . En 2019 esta enfermedad se cobró la vida de más de 400.000 personas, de las cuales un 94 % se concentraban en la región de África. Uno de los principales problemas en el control de la malaria es la aparición de resistencias frente a los diferentes fármacos que existen, es por ello que es necesario el desarrollo de alternativas antimaláricas eficaces. En este estudio se ha aplicado la topología molecular a una serie de compuestos análogos de la 4-anilinoquinolina con actividad inhibitoria de la proliferación de 3 cepas de Plasmodium falciparum, una sensibl…

New active drugs against liver stages of Plasmodium predicted by molecular topology.

2008

ABSTRACT We conducted a quantitative structure-activity relationship (QSAR) study based on a database of 127 compounds previously tested against the liver stage of Plasmodium yoelii in order to develop a model capable of predicting the in vitro antimalarial activities of new compounds. Topological indices were used as structural descriptors, and their relation to antimalarial activity was determined by using linear discriminant analysis. A topological model consisting of two discriminant functions was created. The first function discriminated between active and inactive compounds, and the second identified the most active among the active compounds. The model was then applied sequentially t…

Microbiología alimentaria y fenómenos "paranormales" en la historia

2014

A lo largo de la historia han existido fenómenos aparentemente inexplicables cuyos resultados han originado muertes, milagros, juicios por brujería e incluso ganancias y pérdidas de batallas militares. En este artículo se realiza una inspección microbiológica-histórica en donde algunos microorganismos (Claviceps purpurea, Fusarium sporotrichioides, Serratia marcescens y Stachybotrys chartarum), pueden ser los hipotéticos causantes de algunos de estos fenómenos.

Innovation in bacteriophage-based biocontrol of the plant pathogen ralstonia solanacearum through irrigation water

2017

In silico evaluation of molecular probes for detection and identification of Ralstonia solanacearum and Clavibacter michiganensis subsp. sepedonicus.

2004

Ralstonia solanacerum and Clavibacter michiganensis subsp. sepedonicus are the two most relevant bacterial pathogens of potato for which a large number of molecular diagnostic methods using specific DNA sequences have been developed. About one hundred oligonucleotides have been described and thoroughly tested experimentally. After having compiled and evaluated all these primers and probes in silico to check their specificity, many discrepancies were found. A detailed analysis permitted the recognition of different possible reasons for such discrepancies: sequencing errors in public sequences, wrong supposed specificity (sometimes due to more recent sequences than the oligonucleoticles being…

Survival ofRalstonia solanacearum Biovar 2 in River Water: Influence of Water Microbiota

2008

Current Technologies for Pseudomonas spp. And Ralstonia solanacearum Detection and Molecular Typing

2008

Standard protocols for detection of phytopathogenic Pseudomonas spp. and Ralstonia solanacearum in plant material, soil, water or other sources, often still rely on the isolation of bacterial colonies on appropriate media, and/or on the use of serological techniques. However, over the last several years, molecular techniques, mainly based on PCR methods after extraction of nucleic acids from samples, have improved enough to allow a more rapid, reliable detection of these bacteria. When maximum accuracy is required the use of multiple techniques in an integrated approach is advised. Other promising technologies like flow cytometry, electronic nose or microarrays are emerging due to the need …