Search results for "Atom"

showing 10 items of 31559 documents

Early Commissural Diencephalic Neurons Control Habenular Axon Extension and Targeting.

2016

Summary Most neuronal populations form on both the left and right sides of the brain. Their efferent axons appear to grow synchronously along similar pathways on each side, although the neurons or their environment often differ between the two hemispheres [1–4]. How this coordination is controlled has received little attention. Frequently, neurons establish interhemispheric connections, which can function to integrate information between brain hemispheres (e.g., [5]). Such commissures form very early, suggesting their potential developmental role in coordinating ipsilateral axon navigation during embryonic development [4]. To address the temporal-spatial control of bilateral axon growth, we…

0301 basic medicineEmbryo NonmammalianEfferentNeurogenesisThalamusBiologyTime-Lapse ImagingGeneral Biochemistry Genetics and Molecular Biology03 medical and health sciencesmedicineAnimalsAxonDiencephalonZebrafishZebrafishBody PatterningNeuronsAxon extensionAnatomyCommissureZebrafish Proteinsbiology.organism_classificationAxon growthAxons030104 developmental biologymedicine.anatomical_structureHabenulanervous systemGeneral Agricultural and Biological SciencesNeuroscienceCurrent biology : CB
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Autophagy is required for sea urchin oogenesis and early development.

2016

SummaryAutophagy is a major intracellular pathway for the degradation and recycling of cytosolic components. Emerging evidence has demonstrated its crucial role during the embryo development of invertebrates and vertebrates. We recently demonstrated a massive activation of autophagy in Paracentrotus lividus embryos under cadmium stress conditions, and the existence of a temporal relationship between induced autophagy and apoptosis. Although there have been numerous studies on the role of autophagy in the development of different organisms, information on the autophagic process during oogenesis or at the start of development in marine invertebrates is very limited. Here we report our recent …

0301 basic medicineEmbryo NonmammalianFluorescent Antibody TechniqueCaspase 3ApoptosisFertilization in VitroBiologyParacentrotus lividus03 medical and health sciencesbiology.animalOrganelleBotanyAutophagyAnimalsSettore BIO/06 - Anatomia Comparata E CitologiaSea urchinLC3 Caspase-3 Embryos Oocytes Paracentrotus lividusAutophagyEmbryoCell BiologyMarine invertebratesbiology.organism_classificationCell biology030104 developmental biologyOocytesParacentrotusMacrolidesMicrotubule-Associated ProteinsIntracellularDevelopmental BiologyZygote (Cambridge, England)
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Hsp40 Is Involved in Cilia Regeneration in Sea Urchin Embryos

2003

In a previous paper we demonstrated that, in Paracentrotus lividus embryos, deciliation represents a specific kind of stress that induces an increase in the levels of an acidic protein of about 40 kD (p40). Here we report that deciliation also induces an increase in Hsp40 chaperone levels and enhancement of its ectodermal localization. We suggest that Hsp40 might play a chaperoning role in cilia regeneration.

0301 basic medicineEmbryo NonmammalianHistologyParacentrotus lividus03 medical and health sciences0302 clinical medicineStress PhysiologicalCulture Techniquesbiology.animalEctodermBotanyAnimalsRegenerationElectrophoresis Gel Two-DimensionalCiliaSettore BIO/06 - Anatomia Comparata E CitologiaSea urchinHeat-Shock ProteinsCentrosomebiologyCiliumEmbryoHSP40 Heat-Shock ProteinsSea urchin embryobiology.organism_classificationHsp40 deciliation sea urchinCell biology030104 developmental biologySea UrchinsAnatomy030217 neurology & neurosurgeryMolecular Chaperones
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Non-essential role for cilia in coordinating precise alignment of lens fibres

2016

The primary cilium, a microtubule-based organelle found in most cells, is a centre for mechano-sensing fluid movement and cellular signalling, notably through the Hedgehog pathway. We recently found that each lens fibre cell has an apically situated primary cilium that is polarised to the side of the cell facing the anterior pole of the lens. The direction of polarity is similar in neighbouring cells so that in the global view, lens fibres exhibit planar cell polarity (PCP) along the equatorial-anterior polar axis. Ciliogenesis has been associated with the establishment of PCP, although the exact relationship between PCP and the role of cilia is still controversial. To test the hypothesis t…

0301 basic medicineEmbryologyBBSomeBiologyArticle03 medical and health sciences0302 clinical medicineIntraflagellar transportMicrotubuleCiliogenesisLens CrystallineAnimalsBasal bodyLens placodeCiliaCells CulturedMice KnockoutTumor Suppressor ProteinsCiliumCell PolarityEpithelial CellsAnatomyCell biologyCytoskeletal Proteins030104 developmental biologyFiber cellMicrotubule-Associated Proteins030217 neurology & neurosurgeryDevelopmental BiologyMechanisms of Development
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Dental pulp stem cells for bone tissue engineering: a review of the current literature and a look to the future.

2018

The aim of this narrative review is to investigate the implication of mesenchymal stem cells harvested from human dental pulp in in vivo bone tissue regeneration. We focused on studies related to roles of human dental pulp stem cells in in vivo bone regeneration. A total of 1021 studies were identified; after the assessment of eligibility, only 39 studies were included in the review. The evaluated information of the studies regards the experimental strategies (e.g., the isolation method, the scaffold, the in vivo animal models). The overall main evidences highlighted from the analysis are that dental pulp stem cells and human-exfoliated deciduous teeth stem cells supported by a suitable sc…

0301 basic medicineEmbryologyBiomedical EngineeringDentistryregenerative medicinehuman dental pulpBone tissueRegenerative medicinebone03 medical and health sciences0302 clinical medicinestomatognathic systemTissue engineeringDental pulp stem cellsMedicineBone regenerationbusiness.industryRegeneration (biology)Mesenchymal stem cell030206 dentistrystem cellstomatognathic diseases030104 developmental biologymedicine.anatomical_structuretissue engineeringStem cellbusinessRegenerative medicine
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Heterogeneous nuclear ribonucleoprotein C1 may control miR-30d levels in endometrial exosomes affecting early embryo implantation.

2018

Study question Is there a specific mechanism to load the microRNA (miRNA), hsa-miR-30d, into exosomes to facilitate maternal communication with preimplantation embryos? Summary answer The heterogeneous nuclear ribonucleoprotein C1 (hnRNPC1) is involved in the internalization of endometrial miR-30d into exosomes to prepare for its subsequent incorporation into trophectoderm cells. What is known already Our group previously described a novel cell-to-cell communication mechanism involving the delivery of endometrial miRNAs from the maternal endometrium to the trophectoderm cells of preimplantation embryos. Specifically, human endometrial miR-30d is taken up by murine blastocysts causing the ov…

0301 basic medicineEmbryologyHeterogeneous nuclear ribonucleoproteinBiologyExosomesFlow cytometry03 medical and health sciencesEndometriumMiceTandem Mass SpectrometryGeneticsmedicineAnimalsHumansBlastocystEmbryo ImplantationMolecular Biologymedicine.diagnostic_testHeterogeneous-Nuclear Ribonucleoprotein Group CObstetrics and GynecologyEmbryoCell BiologyTransfectionMolecular biologyEmbryonic stem cellMicrovesiclesCoculture TechniquesBlotMicroRNAs030104 developmental biologymedicine.anatomical_structureReproductive MedicineFemaleDevelopmental BiologyMolecular human reproduction
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Retinal homeobox promotes cell growth, proliferation and survival of mushroom body neuroblasts in the Drosophila brain.

2016

Abstract The Drosophila mushroom bodies, centers of olfactory learning and memory in the fly ‘forebrain’, develop from a set of neural stem cells (neuroblasts) that generate a large number of Kenyon cells (KCs) during sustained cell divisions from embryonic to late pupal stage. We show that retinal homeobox ( rx ), encoding for an evolutionarily conserved transcription factor, is required for proper development of the mushroom bodies. Throughout development rx is expressed in mushroom body neuroblasts (MBNBs), their ganglion mother cells (MB-GMCs) and young KCs. In the absence of rx function, MBNBs form correctly but exhibit a reduction in cell size and mitotic activity, whereas overexpress…

0301 basic medicineEmbryologyanimal structuresNerve Tissue ProteinsBiologyRetina03 medical and health sciencesNeuroblastNeural Stem CellsAnimalsDrosophila ProteinsMitosisMushroom BodiesCell ProliferationGanglion CystsHomeodomain ProteinsNeuronsCell growthfungiCell CycleBrainNuclear ProteinsAnatomyEmbryonic stem cellNeural stem cellCell biologyRepressor Proteins030104 developmental biologyDrosophila melanogasterLarvaMushroom bodiesForebrainHomeoboxDevelopmental BiologyTranscription FactorsMechanisms of development
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Targeting CD52 does not affect murine neuron and microglia function.

2020

The humanized anti-CD52 antibody alemtuzumab is successfully used in the treatment of multiple sclerosis (MS) and is thought to exert most of its therapeutic action by depletion and repopulation of mainly B and T lymphocytes. Although neuroprotective effects of alemtuzumab have been suggested, direct effects of anti-CD52 treatment on glial cells and neurons within the CNS itself have not been investigated so far. Here, we show CD52 expression in murine neurons, astrocytes and microglia, both in vitro and in vivo. As expected, anti CD52-treatment caused profound lymphopenia and improved disease symptoms in mice subjected to experimental autoimmune encephalomyelitis (EAE). CD52 blockade also …

0301 basic medicineEncephalomyelitis Autoimmune ExperimentalCD52Excitotoxicitymedicine.disease_causeNeuroprotection03 medical and health sciencesMice0302 clinical medicinemedicineAnimalsAlemtuzumabPharmacologyNeuronsMicrogliabusiness.industryMultiple sclerosisExperimental autoimmune encephalomyelitismedicine.disease030104 developmental biologymedicine.anatomical_structureCD52 AntigenGene Expression RegulationAlemtuzumabCalciumNeuronMicrogliabusinessNeuroscience030217 neurology & neurosurgerymedicine.drugEuropean journal of pharmacology
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Graph Theoretical Framework of Brain Networks in Multiple Sclerosis: A Review of Concepts.

2019

Abstract Network science provides powerful access to essential organizational principles of the human brain. It has been applied in combination with graph theory to characterize brain connectivity patterns. In multiple sclerosis (MS), analysis of the brain networks derived from either structural or functional imaging provides new insights into pathological processes within the gray and white matter. Beyond focal lesions and diffuse tissue damage, network connectivity patterns could be important for closely tracking and predicting the disease course. In this review, we describe concepts of graph theory, highlight novel issues of tissue reorganization in acute and chronic neuroinflammation an…

0301 basic medicineEncephalomyelitis Autoimmune ExperimentalMultiple SclerosisModels NeurologicalNetwork science03 medical and health sciences0302 clinical medicineNeural PathwaysmedicineAnimalsHumansGeneral NeuroscienceMultiple sclerosisBrainGraph theoryHuman brainmedicine.diseaseFunctional imaging030104 developmental biologymedicine.anatomical_structureGraph (abstract data type)DisconnectionPsychologyNeuroscience030217 neurology & neurosurgeryNetwork analysisNeuroscience
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Hypoxia in CNS Pathologies: Emerging Role of miRNA-Based Neurotherapeutics and Yoga Based Alternative Therapies

2017

Cellular respiration is a vital process for the existence of life. Any condition that results in deprivation of oxygen (also termed as hypoxia) may eventually lead to deleterious effects on the functioning of tissues. Brain being the highest consumer of oxygen is prone to increased risk of hypoxia-induced neurological insults. This in turn has been associated with many diseases of central nervous system (CNS) such as stroke, Alzheimer's, encephalopathy etc. Although several studies have investigated the pathophysiological mechanisms underlying ischemic/hypoxic CNS diseases, the knowledge about protective therapeutic strategies to ameliorate the affected neuronal cells is meager. This has au…

0301 basic medicineEncephalopathyCentral nervous systemIschemiaReviewischemiaBiologyNeuroprotectionlcsh:RC321-571breathing exercise03 medical and health sciencesTherapeutic approach0302 clinical medicinemicroRNAmedicineGene silencinglcsh:Neurosciences. Biological psychiatry. NeuropsychiatrymicroRNAhypoxiaGeneral NeuroscienceHypoxia (medical)medicine.disease030104 developmental biologymedicine.anatomical_structureyoganeuroprotectionmedicine.symptomNeuroscience030217 neurology & neurosurgeryNeuroscienceFrontiers in Neuroscience
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