Search results for "Autograph"

The origins of northern European Autographa gamma individuals evaluated using hydrogen stable isotopes

2018

A novel baculovirus-derived promoter with high activity in the baculovirus expression system

2016

The baculovirus expression vector system (BEVS) has been widely used to produce a large number of recombinant proteins, and is becoming one of the most powerful, robust, and cost-effective systems for the production of eukaryotic proteins. Nevertheless, as in any other protein expression system, it is important to improve the production capabilities of this vector. The orf46 viral gene was identified among the most highly abundant sequences in the transcriptome of Spodoptera exigua larvae infected with its native baculovirus, the S. exigua multiple nucleopolyhedrovirus (SeMNPV). Different sequences upstream of the orf46 gene were cloned, and their promoter activities were tested by the expr…

Functional display of an alpha2 integrin-specific motif (RKK) on the surface of baculovirus particles.

2005

The use of baculovirus vectors shows promise as a tool for gene delivery into mammalian cells. These insect viruses have been shown to transduce a variety of mammalian cell lines, and gene transfer has also been demonstrated in vivo. In this study, we generated two recombinant baculovirus vectors displaying an integrin-specific motif, RKK, as a part of two different loops of the green fluorescent protein (GFP) fused with the major envelope protein gp64 of Autographa californica M nucleopolyhedrovirus. By enzyme linked immunosorbent assays, these viruses were shown to bind a peptide representing the receptor binding site of an α2 integrin, the α2I-domain. However, the interaction was not st…

Atmiņu albumu tradīcija Latvijā sociālajā un kultūras kontekstā

2005

Advisor: Ieva Kalniņa

Advances in Handwriting and Drawing: a multidisciplinary approach

1994

Choix de communications faites à la sixième Conférence internationale sur l'écriture manuscrite et le dessin, Paris, juillet 1993, organisée par l'ENST et l'URA 820 du CNRS; International audience

JAUNIZDALĪTO CEDECEA DAVISAE INFICĒJOŠO BAKTERIOFĀGU IZOLĒŠANA UN RAKSTUROŠANA

2022

Darbā izolētas baktērijas no dažādu rožu dzimtas augļkopības kultūru augu lapām, kuras identificētas ar 16S rRNS gēna sekvenēšanas metodi. Izmantojot šīs baktērijas kā saimniekkultūras, tika izolēti 10 jauni dsDNS bakteriofāgi. Vēlāk ar Cedecea davisae baktēriju izolātu kā saimnieku no notekūdeņu paraugiem tika izolēti vēl 13 fāgi. Tālākai izpētei tika izvēlēts jaunizdalītais Cedecea fāgs Yanou. Fāgs Yanou ir Autographiviridae dzimtai, Teseptimavirus ģintij piederošs lītisks podovīruss ar 40865 bp garu genomu. Tas ir otrs līdz šim zināmais Cedecea ģints fāgs un pirmais raksturotais Cedecea lītiskais podovīruss. Bakteriofāga Yanou pilnais anotētais genoms deponēts GenBank brīvpieejas datubāz…

Host-range expansion of Spodoptera exigua multiple nucleopolyhedrovirus to Agrotis segetum larvae when the midgut is bypassed.

2010

Given the high similarity in genome content and organization between Spodoptera exigua multiple nucleopolyhedrovirus (SeMNPV) and Agrotis segetum nucleopolyhedrovirus (AgseNPV), as well as the high percentages of similarity found between their 30 core genes, the specificity of these NPVs was analysed for the respective insect hosts, S. exigua and A. segetum. The LD(50) for AgseNPV in second-instar A. segetum larvae was 83 occlusion bodies per larva and the LT(50) was 8.1 days. AgseNPV was orally infectious for S. exigua, but the LD(50) was 10 000-fold higher than for SeMNPV. SeMNPV was not infectious for A. segetum larvae when administered orally, but an infection was established by injecti…

Morphological characterization of baculovirus Autographa californica multiple nucleopolyhedrovirus

2009

The budded form of baculovirus Autographa californica multiple nucleopolyhedrovirus is used widely in biotechnological applications. In this study, we observed the morphology of baculovirus in nanometer scale by atomic force microscopy. Additionally, the correlation between transduction efficiency and virus stock storage time was evaluated. By atomic force microscopy, asymmetrical baculovirus particles with enlarged head regions were detected. Observed virus stocks contained variable-length particles, 256 ± 40 nm, along with disintegrated particles and/or cellular components. Long-term storage of stocks led to virus aggregation and decreased cellular entry and transgene expression in mammal…

Baculovirus capsid display: a novel tool for transduction imaging

2003

Baculoviruses are enveloped insect viruses that can carry large quantities of foreign DNA in their genome. Baculoviruses have proved to be very promising gene therapy vectors but little is known about their transduction mechanisms in mammalian cells. We show in this study that Autographa californica multiple nuclear polyhedrosis virus capsid is compatible with the incorporation of desired proteins in large quantities. Fusions can be made to the N-terminus or C-terminus of the major capsid protein vp39 without compromising the viral titer or functionality. As an example of the baculovirus capsid display we show a tracking of the baculovirus transduction in mammalian cells by an enhanced gree…

Baculoviral display of functional scFv and synthetic IgG-binding domains.

2000

Viral vectors displaying specific ligand binding moities such as scFv fragments or intact antibodies hold promise for the development of targeted gene therapy vectors. In this report we describe baculoviral vectors displaying either functional scFv fragments or the synthetic Z/ZZ IgG binding domain derived from protein A. Display on the baculovirus surface was achieved via fusion of the scFv fragment or Z/ZZ domain to the N-terminus of gp64, the major envelope protein of the Autographa californica nuclear polyhedrosis virus, AcNPV. As examples of scFv fragments we have used a murine scFv specific for the hapten 2-phenyloxazolone and a human scFv specific for carcinoembryonic antigen. In pri…