Search results for "BAC"
showing 10 items of 10211 documents
Specialist butterflies benefit most from the ecological restoration of mires
2016
Abstract Anthropogenic disturbances cause biotic homogenization through the replacement of specialist species with generalists. Restoration has the potential to counteract these negative effects. Recently, restoration in the peatlands of Northern Europe has started to show positive effects on biodiversity. However, seldom have studies evaluated the response of insects to restoration by comparing populations prior to restoration to those thereafter with a Before-After Control-Impact (BACI) design. The aim of this study was to evaluate whether the restoration actions taken were appropriate to facilitate the successful recolonization of mire butterflies and plants. We found that, generally, dr…
Periphyton support for littoral secondary production in a highly humic boreal lake
2016
Steep stratification and poor light penetration in highly humic lakes typically restrict oxygenated littoral areas to narrow lake margins. However, in some instances, surrounding floating vegetation mats can sustain highly productive periphyton and more diverse invertebrate communities than pelagic areas. Little is known about how these littoral food webs function or the extent to which the pelagic and littoral food webs are coupled. We added 15N-labeled ammonium nitrate to the floating moss mat surrounding the littoral zone of Mekkojärvi, a small highly humic and fishless lake in southern Finland. Our goal was to increase the δ15N values of periphyton to investigate the diets of littoral i…
Characterization and evolution of two bacteriome-inhabiting symbionts in cixiid planthoppers (Hemiptera: Fulgoromorpha: Pentastirini).
2009
International audience; Like other plant sap-sucking insects, planthoppers within the family Cixiidae (Insecta: Hemiptera: Fulgoromorpha) host a diversified microbiota. We report the identification and first molecular characterization of symbiotic bacteria in cixiid planthoppers (tribe: Pentastirini). Using universal eubacterial primers we first screened the eubacterial 16S rRNA sequences in Pentastiridius leporinus (Linnaeus) with PCR amplification, cloning, and restriction fragment analysis. We identified three main 16S rRNA sequences that corresponded to a Wolbachia bacterium, a plant pathogenic bacterium, and a novel gammaproteobacterial symbiont. A fourth bacterial species affiliated w…
Activation of MAPK homologues by elicitors in tobacco cells
1998
Elicitors of plant defence reactions (such as cryptogein, an elicitin produced by Phytophthora cryptogea, or oligogalacturonides (OGs)), induced in tobacco cell suspensions (Nicotiana tabacum var Xanthi) a rapid and transient activation of two protein kinases (PKs) with apparent molecular masses of 50 and 46 kDa, respectively. These PKs activated and phosphorylated at tyrosine residues, phosphorylated myelin basic protein (MBP) at serine/threonine residues. Both are recognized by anti-MAPK antibodies. The two MBP kinases possessed the same kinetics of activation, and their activation depended, to the same extent, on different exogenously applied compounds (staurosporine, lanthanum, EGTA). W…
The Spodoptera exigua ABCC2 Acts as a Cry1A Receptor Independently of its Nucleotide Binding Domain II
2019
ABC proteins are primary-active transporters that require the binding and hydrolysis of ATP to transport substrates across the membrane. Since the first report of an ABCC2 transporter as receptor of Cry1A toxins, the number of ABC transporters known to be involved in the mode of action of Cry toxins has increased. In Spodoptera exigua, a mutation in the SeABCC2 gene is described as genetically linked to resistance to the Bt-product XentariTM. This mutation affects an intracellular domain involved in ATP binding, but not the extracellular loops. We analyzed whether this mutation affects the role of the SeABCC2 as a functional receptor to Cry1A toxins. The results show that Sf21 cells express…
Larger cell or colony size in winter, smaller in summer – a pattern shared by many species of Lake Kinneret phytoplankton
2017
We examined an 8.5-year record (2004-2012) of cell size data for phytoplankton species from Lake Kinneret, Israel, sampled weekly or at 2-week intervals and determined microscopically by the same person. Many of the species abundant enough to be counted year-round showed a typical seasonal cell size pattern that repeated annually: cell diameter was maximal in winter and minimal in summer. This pattern was shared by species from different taxonomic groups including cyanobacteria, chlorophyta, and dinoflagellates. Similarly, in colonial species of diatoms, chlorophyta, and cyanobacteria the number of cells per colony was larger in winter and smaller in summer. We postulated that the seasonal …
Constitutive expression of clathrin hub hinders elicitor-induced clathrin-mediated endocytosis and defense gene expression in plant cells.
2012
International audience; Endocytosis has been recently implicated in the signaling network associated with the recognition of microbes by plants. In a previous study, we showed that the elicitor cryptogein was able to induce clathrin-mediated endocytosis (CME) in tobacco suspension cells. Herein, we investigate further the induced CME by means of a GFP-tagged clathrin light chain and a CME inhibitor, the hub domain of clathrin heavy chain. Hub constitutive expression does affect neither cell growth nor constitutive endocytosis but abolishes cryptogein-induced CME. Such an inhibition has no impact on early events in the cryptogein signaling pathway but reduces the expression of defense-associ…
Stimulation of Defense Reactions in Medicago truncatula by Antagonistic Lipopeptides from Paenibacillus sp. Strain B2
2010
ABSTRACT With the aim of obtaining new strategies to control plant diseases, we investigated the ability of antagonistic lipopolypeptides (paenimyxin) from Paenibacillus sp. strain B2 to elicit hydrogen peroxide (H 2 O 2 ) production and several defense-related genes in the model legume Medicago truncatula . For this purpose, M. truncatula cell suspensions were used and a pathosystem between M. truncatula and Fusarium acuminatum was established. In M. truncatula cell cultures, the induction of H 2 O 2 reached a maximum 20 min after elicitation with paenimyxin, whereas concentrations higher than 20 μM inhibited H 2 O 2 induction and this was correlated with a lethal effect. In plant roots in…
Innovative, ecofriendly biosorbent-biodegrading biofilms for bioremediation of oil- contaminated water.
2019
Immobilization of microorganisms capable of degrading specific contaminants significantly promotes bioremediation processes. In this study, innovative and ecofriendly biosorbent-biodegrading biofilms have been developed in order to remediate oil-contaminated water. This was achieved by immobilizing hydrocarbon-degrading gammaproteobacteria and actinobacteria on biodegradable oil-adsorbing carriers, based on polylactic acid and polycaprolactone electrospun membranes. High capacities for adhesion and proliferation of bacterial cells were observed by scanning electron microscopy. The bioremediation efficiency of the systems, tested on crude oil and quantified by gas chromatography, showed that…
Fermentation at non-conventional conditions in food- and bio-sciences by the application of advanced processing technologies
2017
The interest in improving the yield and productivity values of relevant microbial fermentations is an increasingly important issue for the scientific community. Therefore, several strategies have been tested for the stimulation of microbial growth and manipulation of their metabolic behavior. One promising approach involves the performance of fermentative processes during non-conventional conditions, which includes high pressure (HP), electric fields (EF) and ultrasound (US). These advanced technologies are usually applied for microbial inactivation in the context of food processing. However, the approach described in this study focuses on the use of these technologies at sub-lethal levels,…