Search results for "BCA"

ITCH E3 ubiquitin ligase downregulation compromises hepatic degradation of branched-chain amino acids

2022

Objective: Metabolic syndrome, obesity, and steatosis are characterized by a range of dysregulations including defects in ubiquitin ligase tagging proteins for degradation. The identification of novel hepatic genes associated with fatty liver disease and metabolic dysregulation may be relevant to unravelling new mechanisms involved in liver disease progression Methods: Through integrative analysis of liver transcriptomic and metabolomic obtained from obese subjects with steatosis, we identified itchy E ubiquitin protein ligase (ITCH) as a gene downregulated in human hepatic tissue in relation to steatosis grade. Wild-type or ITCH knockout mouse models of non-alcoholic fatty liver disease (N…

Structure, interdomain dynamics, and pH-dependent autoactivation of pro-rhodesain, the main lysosomal cysteine protease from African trypanosomes

2021

AbstractRhodesain is the lysosomal cathepsin L-like cysteine protease ofT. brucei rhodesiense, the causative agent of Human African Trypanosomiasis. The enzyme is essential for the proliferation and pathogenicity of the parasite as well as its ability to overcome the blood-brain barrier of the host. Lysosomal cathepsins are expressed as zymogens with an inactivating pro-domain that is cleaved under acidic conditions. A structure of the uncleaved maturation intermediate from a trypanosomal cathepsin L-like protease is currently not available. We thus established the heterologous expression ofT. brucei rhodesiensepro-rhodesain inE. coliand determined its crystal structure. The trypanosomal pr…

A molecular assembly system that renders antigens of choice highly repetitive for induction of protective B cell responses.

2002

Virus like particles (VLPs) are known to induce potent B cell responses in the absence of adjuvants. Moreover, epitope-specific antibody responses may be induced by VLPs that contain peptides inserted in their immunodominant regions. However, due to steric problems, the size of the peptides capable of being incorporated into VLPs while still permitting capsid assembly, is rather limited. While peptides genetically fused to either the N- or C-terminus of VLPs present fewer assembly problems, the immune responses obtained against such epitopes are often limited, most likely because the epitopes are not optimally exposed. In addition, such particles may be less stable in vivo. Here, we show th…

Synthesis and characterization of indium oxide at high pressures

2018

Introducción: La naturaleza es sorprendente pero a la vez limitada. A mi entender, nada tiene más potencial que aplicar el ingenio humano para modificar lo que nos rodea y crear algo completamente nuevo. La Física de la Materia Condensada es un campo que actualmente está ganando importancia en la Física moderna. En virtud de los éxitos logrados en Física de la Materia Condensada se han producido enormes avances en el campo de la electrónica cuántica, de los semiconductores y de la ciencia de materiales, teniendo como resultado numerosas aplicaciones tecnológicas que han cambiado nuestras vidas drásticamente en los últimos 50 años. Una de las ramas de la Física de la Materia Condensada es el…

2020

The efficiency of chemotherapy drugs can be affected by ATP-binding cassette (ABC) transporter expression or by their mutation status. Multidrug resistance is linked with ABC transporter overexpression. In the present study, we performed rare mutation analyses for 12 ABC transporters related to drug resistance (ABCA2, -A3, -B1, -B2, -B5, -C1, -C2, -C3, -C4, -C5, -C6, -G2) in a dataset of 18 cancer patients. We focused on rare mutations resembling tumor heterogeneity of ABC transporters in small tumor subpopulations. Novel rare mutations were found in ABCC1, but not in the other ABC transporters investigated. Diverse ABCC1 mutations were found, including nonsense mutations causing premature …

Downlink channel access performance of NR-U: Impact of numerology and mini-slots on coexistence with Wi-Fi in the 5 GHz band

2020

Coexistence between cellular systems and Wi-Fi gained the attention of the research community when LTE License Assisted Access (LAA) entered the unlicensed band. The recent introduction of NR-U as part of 5G introduces new coexistence opportunities because it implements scalable numerology (flexible subcarrier spacing and OFDM symbol lengths), and non-slot based scheduling (mini-slots), which considerably impact channel access. This paper analyzes the impact of NR-U settings on its coexistence with Wi-Fi networks and compares it with LAA operation using simulations and experiments. First, we propose a downlink channel access simulation model, which addresses the problem of the dependency an…

Distributed Resource Allocation for Energy Efficiency in OFDMA Multicell Networks with Wireless Power Transfer

2019

In this paper, an energy-efficient resource allocation problem is investigated for the wireless power transfer (WPT)-enabled OFDMA multicell networks. In the considered system, multiple base stations (BSs) with a large number of antennas are responsible to provide WPT in the downlink, and the users can recycle and utilize the received energy for uplink data transmission. The role of BS is to execute WPT; thus, there are no data transmissions in the downlink. A time-division protocol is considered to divide the time of downlink WPT and uplink wireless information transfer into separate time slots. With the objective to improve the energy efficiency, we propose the time, subcarrier, and power…

Energy efficient resource allocation for secure OFDMA relay systems with eavesdropper

2016

In this paper, we address the energy efficient resource allocation problem for a secure orthogonal frequency division multiple access (OFDMA) relay system. In particular, we consider there is a eavesdropper near the base station (BS) which tries to overtake the information sent by BS. We formulate the joint optimization problem with the objective to optimize the energy efficiency of the considered system by considering subcarrier pairing, secret data rate and power allocation. In addition, the system can assign different priority to different users such that the security of information transmission can be guaranteed. The proposed iterative algorithm not only maximizes the system energy effi…

New chimaeric hepatitis B virus core particles carrying hantavirus (serotype Puumala) epitopes: immunogenicity and protection against virus challenge

1999

Virus-like particles generated by the heterologous expression of virus structural proteins are able to potentiate the immunogenicity of foreign epitopes presented on their surface. In recent years epitopes of various origin have been inserted into the core antigen of hepatitis B virus (HBV) allowing the formation of chimaeric HBV core particles. Chimaeric core particles carrying the 45 N-terminal amino acids of the Puumala hantavirus nucleocapsid protein induced protective immunity in bank voles, the natural host of this hantavirus. Particles applied in the absence of adjuvant are still immunogenic and partially protective in bank voles. Although a C-terminally truncated core antigen of HBV…

Identification of novel mutations in the ABCA12 gene, c.1857delA and c.5653–5655delTAT, causing harlequin ichthyosis

2013

Abstract Harlequin ichthyosis (HI) is a severe autosomal recessive developmental disorder of the skin that is frequently but not always fatal in the first few days of life. In HI, mutations in both ABCA12 gene alleles must have a severe impact on protein function and most mutations are truncating. The presence of at least one nontruncating mutation (predicting a residual protein function) usually causes a less severe congenital ichthyosis (lamellar ichthyosis or congenital ichthyosiform erythroderma). Here we report on a girl with severe HI diagnosed by prenatal ultrasound at 33 5/7 week gestation. Ultrasound findings included ectropion, eclabium, deformed nose, hands and feet, joint contra…