Search results for "BCE"
showing 10 items of 260 documents
The telomeric Cdc13-Stn1-Ten1 complex regulates RNA polymerase II transcription
2019
Advance article.
Differential occurrence of S100A7 in breast cancer tissues: A proteomic-based investigation
2012
Purpose The present study reports for the first time a large-scale proteomic screening of the occurrence, subcellular localization and relative quantification of the S100A7 protein among a group of 100 patients, clinically grouped for the diagnosis of infiltrating ductal carcinoma (IDC). Experimental design To this purpose, the methods of differential proteomics, Western blotting, and immunohistochemistry were used. Results The identity of two isoforms of the protein was assessed by mass spectrometry and immunologically confirmed. Moreover, we proved by immunocytochemical applications the exclusive localization of the protein within the neoplastic cells. The correlation of S100A7 expression…
The budding yeast Start repressor Whi7 differs in regulation from Whi5, emerging as a major cell cycle brake in response to stress
2020
ABSTRACT Start is the main decision point in the eukaryotic cell cycle at which cells commit to a new round of cell division. It involves the irreversible activation of a transcriptional programme through the inactivation of Start transcriptional repressors: the retinoblastoma family in mammals, or Whi5 and its recently identified paralogue Whi7 (also known as Srl3) in budding yeast. Here, we provide a comprehensive comparison of Whi5 and Whi7 that reveals significant qualitative differences. Indeed, the expression, subcellular localization and functionality of Whi7 and Whi5 are differentially regulated. Importantly, Whi7 shows specific properties in its association with promoters not share…
Convergence of the target of rapamycin and the Snf1 protein kinase pathways in the regulation of the subcellular localization of Msn2, a transcriptio…
2002
The subcellular localization of Msn2, a transcriptional activator of STRE (stress response element)-regulated genes, is modulated by carbon source availability. In cells growing in glucose, Msn2 is located mainly in the cytosol, whereas in carbon source-starved cells, Msn2 is located largely inside the nucleus. However, in cells lacking Reg1 (the regulatory subunit of the Reg1/Glc7 protein phosphatase complex), the regulation of subcellular distribution is absent, Msn2 being constitutively present in the cytosol. The localization defect in these mutants is specific for carbon starvation stress, and it is because of the presence of an abnormally active Snf1 protein kinase that inhibits the n…
A novel Usher protein network at the periciliary reloading point between molecular transport machineries in vertebrate photoreceptor cells.
2008
Contains fulltext : 69178.pdf (Publisher’s version ) (Closed access) The human Usher syndrome (USH) is the most frequent cause of combined deaf-blindness. USH is genetically heterogeneous with at least 12 chromosomal loci assigned to three clinical types, USH1-3. Although these USH types exhibit similar phenotypes in human, the corresponding gene products belong to very different protein classes and families. The scaffold protein harmonin (USH1C) was shown to integrate all identified USH1 and USH2 molecules into protein networks. Here, we analyzed a protein network organized in the absence of harmonin by the scaffold proteins SANS (USH1G) and whirlin (USH2D). Immunoelectron microscopic anal…
A Ferrite Tubular Linear Motor (FTLM): Analysis and design
2015
The electromagnetic linear machines have wide applications in the electromagnetic aircraft launch systems [1] and in transportation systems [2]. Additional energy saving and efficiency can be achieved using a linear motor in the refrigeration application [3]. Linear electromagnetic machines are also very suitable for high-speed packaging, manufacturing sectors and precision positioning. There are different linear motor topologies. Tubular permanent-magnets are particularly attractive since they have a high thrust force density and high efficiency, no end windings, and zero net attractive force between stator and armature. There are various tubular motor topologies, in which the armature may…
Migration of brain capillary endothelial cells inside poly (lactic acid) 3D scaffolds
2017
The brain capillary endothelial cells (BCECs) form the blood brain barrier (BBB) under the effects of the brain microenvironment. BCECs are sealed together by tight junctions (TJs) that are responsible for the barrier phenotype. In these junctions, molecules such as JAM (junctional adhesion molecules), occludin and claudins are present. Threedimensional scaffolds are used to grow cells in order to obtain in vitro engineered tissues. On the base of these considerations, the aim of this work was to understand whether the endothelial cells were able to grow and survive on a new three-dimensional structure. If yes, indeed, this system could be further enriched and used to set a three-dimensiona…
UN «SALTO DI SPECIE» PER L’UE? LA SOLIDARIETA’ EUROPEA ALLA PROVA DELLA CRISI PANDEMICA
2020
La crisi da covid-19 ha riportato al centro del dibattito gli interrogativi sull’effettiva portata della solidarietà europea, quale parte integrante del modello sociale dell’Unione. L’emergenza sanitaria ha mostrato i limiti del processo di integrazione sovranazionale, caratterizzato da un perdurante deficit di legittimazione democratica dell’Unione e dall’assenza di strumenti di condivisione dei rischi tra gli Stati membri. Il paper ripercorre le iniziative prese dalle Istituzioni europee per fronteggiare la crisi pandemica, mettendo in evidenza il cambio di passo dell’Unione rispetto alla precedente crisi finanziaria, e soffermandosi sulle prospettive di approfondimento della sua dimensio…
Prisas intergubernamentales / 4
2004
Este artículo forma un bloque junto a "Ambiciones de un europeísta (1)" (El País, 2 de octubre de 2004); "Matria Europa, 2" (El País, 16 de octubre de 2004) y "El demonio social en la Constitución / 3" (El País, 23 de octubre de 2004).
GFP immunogold staining, from light to electron microscopy, in mammalian cells.
2012
GFP has emerged as an important reporter for monitoring gene expression, protein localization, cell transformation and cell lineage. The development of GFP as a marker in many different biological systems has emphasized the need to image GFP at high resolution. GFP immunogold labeling with colloidal gold particles becomes essential for electron microscopy (EM) ultrastructural detection. Because of the small size, colloidal gold particles require silver enhancement, a procedure to increase the size of the particle as well as gold toning to stabilize the silver layer. GFP preembedding immunogold staining enables high quality cellular-ultrastructural EM analysis mainly for two reasons, on one …