Search results for "BCE"

A novel cholinergic-specific antigen (Chol-2) in mammalian brain.

1993

Three new antisera have been raised in sheep against cholinergic electromotor presynaptic plasma membranes prepared from the electric organs of the electric ray, Torpedo marmorata. They all recognized one or more cholinergic-specific antigens in the mammalian nervous system by the following criteria: they sensitized the cholinergic subpopulation of rat-brain synaptosomes--and only this subpopulation--to lysis by the complement system and, in an immunocytochemical study, selectively stained choline acetyltransferase-positive cholinergic neurons in the rat spinal cord. However, two of the three antisera failed to recognize Chol-1 alpha and -beta, two closely related minor gangliosides already…

Cellular and subcellular distribution of receptors in the entorhinal—hippocampal system: Morphologic and biochemical aspects

1993

Fluctuations and elastic properties of lipid membranes in the gel Lβ′ state: a coarse-grained Monte Carlo study

2010

We study the stress distribution profiles and the height and thickness fluctuations of lipid membranes in the gel L-beta ' state by Monte Carlo simulations of a generic coarse-grained model for lipid membranes, which reproduces many known properties of dipalmitoylphosphatidyncholine (DPPC) bilayers. The results are related to the corresponding properties of fluid membranes, and to theoretical predictions for crystalline and hexatic membranes. One striking observation is that the spontaneous curvature of the monolayers changes sign from the fluid to the gel phase. In the gel-phase, the long-wavelength height fluctuations are suppressed, and the fluctuation spectrum is highly anisotropic. In …

The Uptake of Acetylpyrrolidinecholine — A False Cholinergic Transmitter — Into Mammalian Cerebral Cortical Synaptic Vesicles

1978

It is now well established that cholinergic vesicles are metabolically heterogeneous organelles. It is, however, still under discussion whether this is due to an intravesicular or an intervesicular heterogeneity. March-banks and Israel (10), supporting the hypothesis of intravesicular heterogeneity, suggested that newly formed ACh is only loosely bound to the vesicles. On the other hand, a distinct vesicle fraction obtained from the electromotor system of Torpedo marmorata after its incubation with radioisotopes shows a high specific radioactivity (12), results which favor the view that intervesicular differences do exist. Biochemical and morphological findings suggest that these vesicles h…

ORGANIZATION OF HIGHER-LEVEL CHROMATIN STRUCTURES (CHROMOMERE, CHROMONEMA AND CHROMATIN BLOCK) EXAMINED USING VISIBLE LIGHT-INDUCED CHROMATIN PHOTO-S…

2002

The method of chromatin photo-stabilization by the action of visible light in the presence of ethidium bromide was used for investigation of higher-level chromatin structures in isolated nuclei. As a model we used rat hepatocyte nuclei isolated in buffers which stabilized or destabilized nuclear matrix. Several higher-level chromatin structures were visualized: 100 nm globules—chromomeres, chains of chromomeres—chromonemata, aggregates of chromomeres—blocks of condensed chromatin. All these structures were completely destroyed by 2 M NaCl extraction independent of the matrix state, and DNA was extruded from the residual nuclei (nuclear matrices) into a halo. These results show that nuclear …

El demonio social en la Constitución / 3

2004

Este artículo forma un bloque junto a "Ambiciones de un europeísta (1)" (El País, 2 de octubre de 2004); "Matria Europa, 2" (El País, 16 de octubre de 2004) y "Prisas intergubernamentales / 4" (El País, 30 de obtubre de 2004).

Generalization of a finite-difference numerical method for the steady-state and transient solutions of the nernst—planck flux equations

1985

Abstract A generalization of the numerical method of Brumleve and Buck for the solution of Nernst—Planck equations when convective flux and electric current are involved has been developed. The simulation procedure was applied to a specific case: transport of strong electrolytes in a wide-pore membrane with simultaneous diffusion, convection and electric current. Good agreement was found between experimental data and computed results.

Design and Experimental Test of a Thermomagnetic Motor

2012

Abstract This paper presents a Thermomagnetic Motor. The design of the motor is based on a thermal-magnetic coupled dynamic model, which is obtained by assuming the use of a ferromagnetic material working at temperatures near the curie point. The motor is modeled in terms of both its magnetic as well thermal properties (magnetic permeability and thermal conductivity) and the thermal processes are supposed to be influenced by the thermal conductivity, the convection and the advection. An analytical expression of the generated torque, which links this quantity to the magnetic, thermal and geometrical parameters of the generated torque is given. A design of a machine, based on this theory is p…

Transcriptional repression of Bmp2 by p21(Waf1/Cip1) links quiescence to neural stem cell maintenance.

2013

Relative quiescence and self renewal are defining features of adult stem cells, but their potential coordination remains unclear. Subependymal neural stem cells (NSCs) lacking cyclin-dependent kinase (CDK) inhibitor (CKI) 1a (p21) exhibit rapid expansion that is followed by their permanent loss later in life. Here we demonstrate that transcription of the gene encoding bone morphogenetic protein 2 (Bmp2) in NSCs is under the direct negative control of p21 through actions that are independent of CDK. Loss of p21 in NSCs results in increased levels of secreted BMP2, which induce premature terminal differentiation of multipotent NSCs into mature non-neurogenic astrocytes in an autocrine and/or …

Subcellular localization of pentachlorophenol 4-monooxygenase in Sphingobium chlorophenolicum ATCC 39723.

2002

Abstract We have studied the subcellular localization of pentachlorophenol 4-monooxygenase (PCP4MO) in Sphingobium chlorophenolicum ATCC 39723 during induction by pentachlorophenol (PCP). Using a monoclonal antibody CL6 specific to the native and recombinant PCP4MO, the enzyme was primarily found soluble as determined by immunoblot and ELISA analyses of cellular fractions. However, the enzyme was observed both in the soluble and membrane-bound forms during induction for 2–4 h, suggesting its translocation out from the cytoplasm. Electron microscopy confirmed that PCP4MO was predominantly present in the cytoplasm at 1 h, whereas at 4 h significant amount was detected also in the membrane and…