Search results for "BCE"
showing 10 items of 260 documents
Subcellular fractionation of tissue culture cells.
2003
Cell fractionation techniques include some of the most important and widely used analytical tools in cell and molecular biology, and are essential for the development of cell-free assays that reconstitute complicated cellular processes. In addition to simple gradient systems, this unit discusses the immuno-purification of organelles, in particular endosomes. As antigens, purification can be achieved using endogenous or ectopically expressed proteins, provided that appropriate antibodies are available. Alternatively, tagged proteins can be used, when combined with anti-tag antibodies. Now that sequencing of the genomes of several organisms has been completed, biochemical strategies, and in p…
Characterization of the cleavage site and function of resulting cleavage fragments after limited proteolysis of Clostridium difficile toxin B (TcdB) …
2005
Clostridium difficiletoxin B (TcdB) is a single-stranded protein consisting of a C-terminal domain responsible for binding to the host cell membrane, a middle part involved in internalization, and the N-terminal catalytic (toxic) part. This study shows that TcdB is processed by a single proteolytic step which cleaves TcdB10463between Leu543and Gly544and the naturally occurring variant TcdB8864between Leu544and Gly545. The cleavage occurs at neutral pH and is catalysed by a pepstatin-sensitive protease localized in the cytoplasm and on the cytoplasmic face of intracellular membranes. The smaller N-terminal cleavage products [63 121 Da (TcdB10463) and 62 761 Da (TcdB8864)] harbour the cytotox…
ROTOR FLUX OPTIMAL ESTIMATION FOR INDUCTION MOTOR CONTROL
2005
Abstract The aim of this paper is to analyze and design reduced order observers of the rotor flux of induction motors. The design requirements are: a) the convergence rate of the rotor flux estimation error; b) a low sensitivity to stator and rotor resistance variations; c) a low sensitivity to errors due to the implementation of the observers on microprocessor-based systems. It is shown that, in order to satisfy the requirements a)-c), it is sufficient to solve a constrained optimization problem according to a criterion in which these requirements appear explicitly. The implementation of the observer is discussed. The observer is tested by simulation and experiments.
Interior permanent magnet synchronous motors: Impact of the variability of the parameters on their efficiency
2016
In the scientific literature, various authors presented different control strategies aimed to increase the efficiency of the interior permanent magnet synchronous motors. These strategies, starting from a well-known mathematical model of the dynamic behaviour of the motor, are able to reduce the power losses acting on the magnetizing component of the stator current. However, none of the proposed approaches takes into account that, varying the working conditions of the motor, also the values of the parameters, which have an impact on the power losses, can vary. In this paper, therefore, starting from an accurate measurement of the motor characteristics for various values of speed and load, w…
Closed-loop bandwidth impact on MVSA for rotor broken bar diagnosis in IRFOC double squirrel cage induction motor drives
2013
This paper investigates the detectability of rotor broken bars in indirect rotor flux oriented control (IRFOC) for variable speed double cage induction motor drives, using vibration signature analysis techniques. The Impact of the closed loop control system cannot be neglected when the detection of rotor asymmetries in the machine is based on the signature analysis of electrical or mechanical variables. Therefore, the investigation of rotor fault components for different bandwidths of closed-loop regulators is necessary to evaluate its relevance in the above listed variables. This paper investigates the impact of the control system on relevance of the fault components computed from axial an…
Differential subcellular localization of endogenous and transfected soluble epoxide hydrolase in mammalian cells: evidence for isozyme variants
1999
AbstractEndogenous, constitutive soluble epoxide hydrolase in mice 3T3 cells was localized via immunofluorescence microscopy exclusively in peroxisomes, whereas transiently expressed mouse soluble epoxide hydrolase (from clofibrate-treated liver) accumulated only in the cytosol of 3T3 and HeLa cells. When the C-terminal Ile of mouse soluble epoxide hydrolase was mutated to generate a prototypic putative type 1 PTS (-SKI to -SKL), the enzyme targeted to peroxisomes. The possibility that soluble epoxide hydrolase-SKI was sorted slowly to peroxiosmes from the cytosol was examined by stably expressing rat soluble epoxide hydrolase-SKI appended to the green fluorescent protein. Green fluorescent…
Intra-urban spatial distributions of population and employment : the case of the agglomeration of Dijon, 1999
2003
The aim of this paper is to analyze the intra-urban spatial distributions of population and employment in the agglomeration of Dijon (regional capital of Burgundy, France). We study whether this agglomeration has followed the general tendency of job decentralization observed in most urban areas or whether it is still characterized by a monocentric pattern. In that purpose, we use a sample of 136 observations at the communal and at the IRIS (infra-urban statistical area) levels with 1999 census data and the employment database SIRENE (INSEE). First; we study the spatial pattern of total employment and employment density using exploratory spatial data analysis. Apart from the CBD, few IRIS ar…
Caracterización de la familia génica LGI
2010
158 pages.
Efectos de la cocaína y del tolueno sobre la neurogénesis en el hipocampo de rata
2009
En el presente proyecto se estudió el efecto de dos drogas adictivas, el tolueno y la cocaína, sobre la neurogénesis hipocámpica. Los experimentos diseñados para ambas drogas, abordaron el estudio de la neurogénesis, a través de la medida de proliferación, supervivencia, diferenciación y migración. Además se analizó el efecto ultraestructural que ambas drogas causan sobre el giro dentado. De forma más exhaustiva, se estudió el efecto producido por una administración crónica del tolueno, durante el desarrollo del animal. Por último, se estudiaron los efectos de este tratamiento sobre la memoria y el aprendizaje. En los experimentos con cocaína, se estudió el efecto sobre la proliferación, su…
Fasciolosis crónica avanzada: análisis del desarrollo del parásito adulto y de la oviposición en el modelo experimental Wistar
2009
La patogénesis de la fasciolosis depende de la carga parasitaria y del tamaño de los trematodos adultos. En zonas de hiperendemia, las intensidades individuales de parasitación humana pueden alcanzar niveles muy altos, estando la mayoría de los individuos parasitados en la fase de cronicidad avanzada. En este estudio se ha utilizado el modelo experimental de fasciolosis en rata Wistar por ser un modelo animal útil que mimetiza la fasciolosis crónica avanzada en humanos. Se ha infectado un total de 169 ratas con 20 metacercarias/rata vía oral, llevándose a cabo los siguientes análisis: A) Estudio la influencia de la intensidad individual de infección por rata (entendiendo la intensidad de in…