Search results for "BIOLOGIE MOLECULAIRE"
showing 10 items of 29 documents
Molecular analysis of the nitrate-reducing community from unplanted and maize-planted soils
2002
ABSTRACT Microorganisms that use nitrate as an alternative terminal electron acceptor play an important role in the global nitrogen cycle. The diversity of the nitrate-reducing community in soil and the influence of the maize roots on the structure of this community were studied. The narG gene encoding the membrane bound nitrate reductase was selected as a functional marker for the nitrate-reducing community. The use of narG is of special interest because the phylogeny of the narG gene closely reflects the 16S ribosomal DNA phylogeny. Therefore, targeting the narG gene provided for the first time a unique insight into the taxonomic composition of the nitrate-reducing community in planted an…
Mecanismes moleculaires de pathogenicite des Escherichia coli inducteurs de diarrhees chez l'homme et l'animal
1993
Inhibition of in vitro reconstitution of rotavirus transcriptionally active particles by anti-VP6 monoclonal antibodies
1994
International audience; Six monoclonal antibodies specific for the major capsid protein of rotavirus, VP6, previously characterized, were tested in a biological assay for their capacity to block the transcriptase activity associated with the single-shelled particles. The results showed that two MAbs (RV-50 and RV-133), specific for distinct antigenic sites, were able to block the transcription when they were incubated with a purified baculovirus-expressed group A VP6, prior to the reconstitution of the single-shelled particles from the cores, suggesting that at least two domains are involved in active single-shelled particle reconstitution. The results obtained previously from immunochemist…
Sampling strategy in molecular microbial ecology: influence of soil sample size on DNA fingerprinting analysis of fungal and bacterial communities.
2003
Assessing soil microbial community structure by the use of molecular techniques requires a satisfactory sampling strategy that takes into account the high microbial diversity and the heterogeneous distribution of microorganisms in the soil matrix. The influence of the sample size of three different soil types (sand, silt and clay soils) on the DNA yield and analysis of bacterial and fungal community structure were investigated. Six sample sizes from 0.125 g to 4 g were evaluated. The genetic community structure was assessed by automated ribosomal intergenic spacer analysis (A-RISA fingerprint). Variations between bacterial (B-ARISA) and fungal (F-ARISA) community structure were quantified b…
Résistance aux herbicides chez le vulpin et le ray-grass : des marqueurs moléculaires pour un diagnostic rapide
2002
National audience
Résistance aux herbicides chez le vulpin
2003
National audience
Obtention d'anticorps monoclonaux polyvalents utilisables pour la détection ELISA du Plum pox virus (PPV) en utilisant un peptide synthétique représe…
2005
National audience
Map gene as a new non ribosomal marker to study the molecular variability of grapevine yellows and related phytoplasmas from group 16SRV in Europe
2006
International audience
Sequence typing of map, secY and degV genes confirm the genetic relatedness of three distinct groups of flavescence dorée phytoplasmas with phytoplas…
2006
International audience
Criteres moleculaires impliques dans la relation abeille-plante : une approche biologique et chimique
1987
National audience