Search results for "BLAST"
showing 10 items of 2136 documents
Differential incorporation of labeled amino acids in the territories of the sea urchin blastula
1962
Blastule con mesenchima primario diParacentrotus lividus esposte a un trattamento di 30 min con S35-metionina, leucina-C14 e alanina-C14 mostrano nel territorio vegetativo una densita di tracce considerevolmente minore che in quello animale. Se gli embrioni sono invece esposti all'aminoacido radioattivo durante tutto lo sviluppo o durante le prime ore dopo la fecondazione, tale differenza tra territori animale e vegetativo non si osserva. Si discute il significato di questi reperti.
2,3,7,8-Tetrachlorodibenzo-p-dioxin-Dependent Release from Contact Inhibition in WB-F344 Cells: Involvement of Cyclin A
2002
2,3,7,8-Tetrachlorodibenzo-p-dioxin (TCDD) is the most potent tumor promoter ever tested in rodents. Although it is known that most of TCDD actions are mediated by binding to the aryl hydrocarbon receptor (AhR), the mechanisms leading to tumor promotion still remain to be elucidated. Loss of contact inhibition is one characteristic hallmark in tumorigenesis. In rat liver epithelial WB-F344 cells, TCDD induces a release from contact inhibition, which is manifested by a twofold increase in cell number when TCDD (1 nM for 48 h) is added to confluent cells in the presence of serum, but not when given to exponentially growing or subconfluent, serum-deprived WB-F344 cells. Loss of G1 arrest was a…
Gene amplification in fibroblasts from ataxia telangiectasia (AT) patients and in X-ray hypersensitive AT-like Chinese hamster mutants.
2001
In search of functions involved in the regulation of gene amplification, and given the relevance of chromosome breakage in initiating the process, we analyzed the gene amplification ability of cells hypersensitive to inducers of DNA double-strand breaks and defective in cell cycle control: two human fibroblast strains derived from patients affected by ataxia telangiectasia (AT) and two hamster mutant cell lines belonging to complementation group XRCC8 of the rodent X-ray-sensitive mutants. These mutants are considered hamster models of AT cells. To measure gene amplification, the frequency and the rate of occurrence of N-(phosphonacetyl)-L-aspartate resistant cells were determined. In both …
Differential gene expression in p53-mediated G(1) arrest of human fibroblasts after gamma-irradiation or N-phosphoacetyl-L-aspartate treatment.
2000
In human fibroblasts, N:-phosphoacetyl-L-aspartate (PALA) and gamma-radiation induce reversible and irreversible p53-mediated G(1) cell cycle arrest, respectively. By coupling the premature chromosome condensation technique to fluorescence in situ hybridization, we found no evidence of DNA damage after PALA treatment. We used representational difference analysis (cDNA-RDA) to study changes in gene expression after PALA treatment and gamma-radiation in normal human fibroblasts. The mammary-derived growth inhibitor (MDGI) gene was expressed in PALA-treated cells. Ectopic MDGI expression arrested PALA-treated but not irradiated RKO cells. Expression of an antisense RNA against MDGI resulted in…
Symmetry Breaking and Establishment of Dorsal/Ventral Polarity in the Early Sea Urchin Embryo
2015
The mechanisms imposing the Dorsal/Ventral (DV) polarity of the early sea urchin embryo consist of a combination of inherited maternal information and inductive interactions among blastomeres. Old and recent studies suggest that a key molecular landmark of DV polarization is the expression of nodal on the future ventral side, in apparent contrast with other metazoan embryos, where nodal is expressed dorsally. A subtle maternally-inherited redox anisotropy, plus some maternal factors such as SoxB1, Univin, and p38-MAPK have been identified as inputs driving the spatially asymmetric transcription of nodal. However, all the mentioned factors are broadly distributed in the embryo as early as no…
Gamma-ray burst afterglow light curves from realistic density profiles
2011
The afterglow emission that follows gamma-ray bursts (GRBs) contains valuable information about the circumburst medium and, therefore, about the GRB progenitor. Theoretical studies of GRB blast waves, however, are often limited to simple density profiles for the external medium (mostly constant density and power-law R^{-k} ones). We argue that a large fraction of long-duration GRBs should take place in massive stellar clusters where the circumburst medium is much more complicated. As a case study, we simulate the propagation of a GRB blast wave in a medium shaped by the collision of the winds of O and Wolf-Rayet stars, the typical distance of which is d /sim 0.1 - 1 pc. Assuming a spherical…
Hydrodynamic simulations of the interaction of supernova shock waves with a clumpy environment: the case of the RX J0852.0-4622 (Vela Jr.) supernova …
2013
Observations in all electromagnetic bands show that many supernova remnants (SNRs) have a very aspherical shape. This can be the result of asymmetries in the supernova explosion or a clumpy circumstellar medium. We study the generation of inhomogeneities and the mixing of elements arising from these two sources in multidimensional hydrodynamic simulations of the propagation of a supernova blast wave into a cloudy environment. We model a specific SNR, Vela Jr (RX J0852.0-4622). By comparing our results with recent observations, we can constrain the properties of the explosion. We find that a very energetic explosion of several 10^{51} erg occurring roughly about 800 years ago is consistent w…
X-RAY SPECTROSCOPIC DIAGNOSIS OF A WIND-COLLIMATED BLAST WAVE AND METAL-RICH EJECTA FROM THE 2006 EXPLOSION OF RS OPHIUCHI
2009
Chandra HETG observations of RS Ophiuchi at day 13.9 of the 2006 outburst reveal a rich spectrum of emission lines from abundant ions formed over a wide temperature range (∼ 3 × 10 6 to 60 × 10 6 K) indicative of shock heating of the circumstellar medium by the expanding blast wave. Lines are asymmetric and strongly broadened (v ∼ 2400 km s −1 at zero intensity). Using simple analytical model profiles, we show how the lines are shaped by differential absorption in the red giant wind and explosion ejecta, and that shock heating to multimillion degree temperatures appears to have occurred preferentially in the direction perpendicular to the line of sight. We conclude that the asymmetric natur…
Do Intestinal Unicellular Parasites Have a Role in the Inflammatory and Redox Status among the Severely Obese?
2022
The diagnosis of obesity comprises subjects with totally different phenotypes and metabolic profiles. Systemic inflammation and oxidative stress derived from the white adipose tissue are suggested as the link between this disease and the development of insulin resistance and metabolic comorbidities. The presence of unicellular eukaryotic parasites colonizing the human gut ecosystem is a common circumstance, and yet their influence on the inflammatory and redox status of the obese host has not been assessed. Herein, a set of inflammatory and redox biomarkers were assessed together with a parasitological analysis of 97 severely obese subjects. Information was also collected on insulin resista…
The depletion of nuclear glutathione impairs cell proliferation in 3t3 fibroblasts.
2009
BACKGROUND:Glutathione is considered essential for survival in mammalian cells and yeast but not in prokaryotic cells. The presence of a nuclear pool of glutathione has been demonstrated but its role in cellular proliferation and differentiation is still a matter of debate. PRINCIPAL FINDINGS:We have studied proliferation of 3T3 fibroblasts for a period of 5 days. Cells were treated with two well known depleting agents, diethyl maleate (DEM) and buthionine sulfoximine (BSO), and the cellular and nuclear glutathione levels were assessed by analytical and confocal microscopic techniques, respectively. Both agents decreased total cellular glutathione although depletion by BSO was more sustaine…