Search results for "BLAST"

showing 10 items of 2136 documents

Plasma and fibroblasts of Tangier disease patients are disturbed in transferring phospholipids onto apolipoprotein A-I

1998

Plasmas of patients with Tangier disease (TD) lack lipid-rich α-HDL which, in normal plasma, constitutes the majority of high density lipoprotein (HDL). Residual amounts of apolipoprotein (apo)A-I in TD plasma occur as lipid-poor or even lipid-free preβ-HDL. By contrast to normal plasma, TD plasma does not convert preβ-HDL into α-HDL. Moreover, fibroblasts of TD patients were found to be defective in secreting cholesterol or phospholipids in the presence of lipid-free apoA-I. We have therefore hypothesized that both defective conversion of preβ-HDL into α-HDL and defective lipid efflux from TD cells onto lipid-free apoA-I result from a disturbance in phospholipid transfer occurring in both …

AdultMaletransferring phospholipidsPhospholipidTangier diseasePhosphatidic AcidsQD415-436PhosphatidylinositolsBiochemistrychemistry.chemical_compoundEndocrinologyTangier diseasePhosphatidylcholinePhospholipid transfer proteinExtracellularmedicineHumansCells CulturedPhosphatidylethanolamineApolipoprotein A-ICholesterolPhosphatidylethanolaminesReverse cholesterol transportnutritional and metabolic diseasesBiological TransportCell BiologyFibroblastsmedicine.diseaseMolecular biologyfamilial HDL deficiencyreverse cholesterol transportLipoproteins LDLphospholipid transfer proteinsprebeta-HDLTangier disease; transferring phospholipidschemistryPhosphatidylcholinesFemalelipids (amino acids peptides and proteins)cholesterol efflux
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Repair of the mandibular nerve by means of autologous nerve grafting after resection of the lower jaw

1973

Summary On the basis of two cases we demonstrate our method of autologous nerve grafting for substitution of the mandibular nerve after mandibular resection. The sural nerve served as a donor nerve, the graft was imbedded microsurgically at the juncture points by means of a perineurial interfascicular nerve suture. Oversized grafts of about 20 cm in length were chosen intentionally in order to insert them without tension between the stumps of the recipient nerve and outside the regeneration zone of the bone. In both cases complete resensibilization of the lower lip was reached after about six months.

AdultMicrosurgerymedicine.medical_specialtyMandibular NerveMandibular nerveLower lipSural nerveTransplantation AutologousResectionAmeloblastomaPostoperative ComplicationsSural Nervestomatognathic systemmedicineHumansParesthesiaChildNerve graftingbusiness.industryGeneral MedicineAnatomyDenervationMandibular resectionLipNerve RegenerationOsteotomySurgeryMandibular Neoplasmssurgical procedures operativeFemaleSurgeryEpineurial repairNerve suturebusinessJournal of Maxillofacial Surgery
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Oral versus intravenous vinorelbine: clinical safety profile

2005

The availability of chemotherapeutic drugs administrable by oral route represents a step forward in the management of cancer patients. Among oral agents, vinorelbine is particularly interesting for its pharmacological characteristics and clinical efficacy. Oral vinorelbine is rapidly absorbed (1.5-3 hours) with an elimination half-life of approximately 40 hours. It shows a low level of binding to plasma proteins (13%), is highly bound to platelets (78%) and has a hepatic metabolism and an absolute bioavailability of 40% with a moderate and similar interpatient variability for the two forms. Food has no influence on the pharmacokinetic profile of oral vinorelbine even if nausea/vomiting is l…

AdultNauseaAdministration OralBiological AvailabilityPharmacologyVinblastineVinorelbineAbsorptionEatingTherapeutic indexCytochrome P-450 Enzyme SystemPharmacokineticsOral administrationNeoplasmsmedicineHumansPharmacology (medical)Infusions IntravenousAgedbreast cancer non-small cell lung cancer (NSCLC) oral vinorelbinebusiness.industryStandard treatmentAge FactorsVinorelbineGeneral MedicineMiddle AgedAntineoplastic Agents PhytogenicLiverVomitingmedicine.symptombusinessDrug metabolismHalf-Lifemedicine.drug
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Investigating the Antioxidant and Cytocompatibility of Mimusops elengi Linn Extract over Human Gingival Fibroblast Cells

2021

Background—chlorhexidine (CHX) is most commonly used as a chemical plaque control agent. Nevertheless, its adverse effects, including teeth discoloration, taste alteration and calculus build-up, limit its use and divert us to medicinal herbs. The purpose of the study was to evaluate the phytochemical composition, antioxidant potential, and cytotoxic effects of Mimusops elengi Linn extract (ME) over normal human cultured adult gingival fibroblasts (HGFs). Methods—in vitro phytochemical screening, total flavonoid content, antioxidant potential by DPPH and Nitric Oxide (NO) radical scavenging activity, and cytotoxic effects of ME extracts over HGF were explored. The viability of HGF cells was …

AdultNeutral redAntioxidantDPPHHealth Toxicology and Mutagenesismedicine.medical_treatment02 engineering and technologyMimusops elengiPharmacologyMimusopsArticleAntioxidantsfibroblast03 medical and health scienceschemistry.chemical_compound0302 clinical medicinemedicineHumansMTT assayViability assaymedicinalbiologyPlant ExtractschlorhexidinePublic Health Environmental and Occupational HealthR030206 dentistryherbsFibroblasts021001 nanoscience & nanotechnologybiology.organism_classificationPhytochemicalchemistrygingivalMedicinecytotoxicityTrypan blue0210 nano-technologyInternational Journal of Environmental Research and Public Health
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Vinorelbine and Cisplatin for the Treatment of Recurrent and/or Metastatic Carcinoma of the Uterine Cervix

2002

<i>Background:</i> To test the clinical activity and toxicity profile of the combination regimen of vinorelbine and cisplatin in a series of patients with carcinoma of the cervix uteri with de novo metastatic disease or recurrent disease after previous therapy. The main aims of the study included analysis of objective response rates, toxicity, and time to progression. <i>Patients and Methods:</i> Forty-two eligible patients were enrolled into the trial and treated with cisplatin 80 mg/m<sup>2</sup> on day 1 and vinorelbine 25 mg/m<sup>2</sup> on day 1 and 8. This regimen was repeated every 21 days upon resolution of toxicity for 3 cycles befor…

AdultOncologyCancer Researchmedicine.medical_specialtymedicine.medical_treatmentUterine Cervical NeoplasmsVinblastineVinorelbineMetastatic carcinomaRecurrenceInternal medicineAntineoplastic Combined Chemotherapy ProtocolsCarcinomaHumansMedicineNeoplasm MetastasisCervixAgedCisplatinChemotherapyurogenital systembusiness.industryVinorelbineGeneral MedicineMiddle Agedmedicine.diseaseSurgeryRegimenTreatment Outcomemedicine.anatomical_structureOncologyAdenocarcinomaFemaleCisplatinbusinessmedicine.drugOncology
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Favorable long-term follow-up results over 6 years for response, survival, and safety with imatinib mesylate therapy in chronic-phase chronic myeloid…

2008

Abstract Imatinib mesylate, a targeted inhibitor of BCR-ABL tyrosine kinase, is the standard of care for chronic myeloid leukemia (CML). A phase 2 trial of imatinib in late chronic-phase (CP) CML after interferon-α (IFNα) failure enrolled 532 patients, 454 with a confirmed diagnosis of CP CML. Median time from diagnosis was 34 months; median duration of imatinib treatment was 65 months. Cumulative best rates of major cytogenetic response (MCyR) and complete cytogenetic response (CCyR) were 67% and 57%, respectively. At the 5-year landmark, 184 (41%) of the 454 patients are in CCyR. At more than 6 years, 199 (44%) of the 454 patients remain on imatinib. Most responses occurred within 12 mont…

AdultOncologymedicine.medical_specialtyTime FactorsAdolescentDrug-Related Side Effects and Adverse Reactionsmedicine.drug_classImmunologyimatinib CML interferon-alphaSalvage therapyBlastic PhaseBiochemistryPiperazinesTyrosine-kinase inhibitorhemic and lymphatic diseasesInternal medicinemedicineHumansneoplasmsSurvival rateAgedAged 80 and overSalvage Therapybusiness.industryInterferon-alphaMyeloid leukemiaImatinibCell BiologyHematologyMiddle Agedmedicine.diseaseSurgerySurvival RatePyrimidinesTreatment OutcomeImatinib mesylateBenzamidesLeukemia Myeloid Chronic-PhaseDisease ProgressionImatinib MesylatebusinessFollow-Up StudiesChronic myelogenous leukemiamedicine.drug
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Outcome of peripheral blood stem cell mobilization in advanced phases of CML is dependent on the type of chemotherapy applied

1998

High-dose chemotherapy with autologous transplantation of in vivo purged PBSC is a novel investigational approach to treating chronic myelogenous leukemia (CML) patients not responsive to conventional therapy with interferon-alpha (IFN-alpha) and not eligible for allogeneic transplantation. PBSC mobilization using either '5+2/7+3'-type chemotherapy or 'mini-ICE/ ICE' chemotherapy was investigated in 43 patients with advanced phases of Philadelphia (Ph)-positive CML. Thirty patients were in late chronic phase (12 months post diagnosis) and 13 patients in accelerated phase (AP) or blast crisis (BC). Contamination with Ph-positive cells was evaluated in harvests from 37/43 patients. The outcom…

AdultOncologymedicine.medical_specialtyTime FactorsAllogeneic transplantationmedicine.medical_treatmentPilot ProjectsCarboplatinCohort StudiesLeukemia Myelogenous Chronic BCR-ABL PositiveInternal medicineAntineoplastic Combined Chemotherapy ProtocolsmedicineHumansAutologous transplantationIfosfamideEtoposideChemotherapyMobilizationHematologybusiness.industryHematologyGeneral MedicineLeukapheresisMiddle Agedmedicine.diseaseHematopoietic Stem Cell MobilizationSurgeryTreatment OutcomeBlast CrisisComplicationbusinessChronic myelogenous leukemiaAnnals of Hematology
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Wilms' tumor in patients with 9q22.3 microdeletion syndrome suggests a role for PTCH1 in nephroblastomas

2012

Nephroblastoma (Wilms' tumor; WT) is the most common renal tumor of childhood. To date, several genetic abnormalities predisposing to WT have been identified in rare overgrowth syndromes. Among them, abnormal methylation of the 11p15 region, GPC3 and DIS3L2 mutations, which are responsible for Beckwith-Wiedemann, Simpson-Golabi-Behmel and Perlman syndromes, respectively. However, the underlying cause of WT remains unknown in the majority of cases. We report three unrelated patients who presented with WT in addition to a constitutional 9q22.3 microdeletion and dysmorphic/overgrowth syndrome. The size of the deletions was variable (ie, from 1.7 to 8.9 Mb) but invariably encompassed the PTCH1 …

AdultPatched Receptorsmedicine.medical_specialtyPathologyPTCH1AdolescentNonsense mutationCNVShort ReportReceptors Cell SurfaceBiologymedicine.disease_causeWilms’ tumorWilms TumorFetal MacrosomiaSettore MED/38 - Pediatria Generale E SpecialisticaPregnancyInternal medicineGeneticsmedicineHumansPerlman syndromeChildovergrowthGenetics (clinical)MutationComparative Genomic HybridizationWilms' tumorPTCH1 GeneMicrodeletion syndromeFANCC nephroblastomamedicine.diseaseKidney NeoplasmsPatched-1 ReceptorEndocrinologyPTCH1Settore MED/03 - Genetica MedicaOvergrowth syndromeMutationFemaleChromosome DeletionChromosomes Human Pair 9Comparative genomic hybridization
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The role of osteonectin in human tooth development: An immunohistological study

1992

We investigated immunohistologically 160 teeth and dental germs in various stages of tooth development taken from human individuals (13th week of pregnancy to the 24th year of life) to study the osteonectin expression in dental hard tissue. In the course of dentinogenesis, the predentin, the odontoblasts, and their cell processes show a positive osteonectin staining reaction. During cementogenesis, osteonectin is synthesized by cement-producing fibroblasts, cementoblasts, and cementocytes. The expression of osteonectin during dentinogenesis and cementogenesis is closely related to the development of the respective calcified tissue. All cells of the inner and outer enamel epithelium, the cel…

AdultPathologymedicine.medical_specialtyAdolescentEndocrinology Diabetes and MetabolismDentistryStratum intermediumFetusEndocrinologystomatognathic systemAmelogenesisHuman tooth developmentmedicineHumansOsteonectinOrthopedics and Sports MedicineChildDental Cementumbiologybusiness.industryChemistryInfant NewbornInfantAmelogenesisDentinogenesisFibroblastsmusculoskeletal systemImmunohistochemistryCementogenesisstomatognathic diseasesOdontoblastChild PreschoolDentinogenesisbiology.proteinOsteonectinbusinessAmeloblastToothCalcified Tissue International
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Induction of bone morphogenetic protein-6 in skin wounds. Delayed reepitheliazation and scar formation in BMP-6 overexpressing transgenic mice.

1998

Growth factors of the transforming growth factor-beta superfamily are involved in cutaneous wound healing. In this study we analyze the expression of the bone morphogenetic protein-6 (BMP-6) gene, a transforming growth factor-beta related gene, in skin wounds. In normal mouse skin high levels of BMP-6 mRNA and protein are expressed by postmitotic keratinocytes of stratified epidermis until day 6 after birth. BMP-6 expression is strongly reduced in adult epidermis with diminished mitotic activity. After skin injury we found large induction of BMP-6-specific RNA and protein in keratinocytes at the wound edge and keratinocytes of the newly formed epithelium as well as in fibroblast shaped cell…

AdultPathologymedicine.medical_specialtyBone Morphogenetic Protein 6Gene ExpressionMice TransgenicDermatologyBiologyBiochemistryMiceTransforming Growth Factor betaGene expressionmedicineAnimalsHumansFibroblastMolecular BiologySkinMessenger RNAWound Healingintegumentary systemEpidermis (botany)RNACell DifferentiationCell BiologyCell biologyUp-RegulationBone morphogenetic protein 6medicine.anatomical_structureBone Morphogenetic ProteinsRNAWounds and InjuriesWound healingCell DivisionTransforming growth factorThe Journal of investigative dermatology
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