Search results for "BLAST"

Depletion of alphaV integrins from osteosarcoma cells by intracellular antibody expression induces bone differentiation marker genes and suppresses g…

1999

Integrin heterodimers sharing the common alphaV subunit are receptors for adhesion glycoproteins such as vitronectin and fibronectin. They are suggested to play an essential role in cell anchoring, differentiation, and survival. Here, we describe the construction of an expression plasmid coding for an intracellular single-chain antibody against alphaV integrin subunit. Saos-2 osteosarcoma cells transfected with this DNA construct showed an approximately 70-100% decrease in the cell surface expression of alphaVbeta3 and alphaVbeta5 integrins as shown by flow cytometry. Intracellular antibody expression had no effect on the mRNA levels of alphaV integrin. Pulse chase experiments of metabolica…

Interaction of antibodies to proteinase 3 (classic anti-neutrophil cytoplasmic antibody) with human renal tubular epithelial cells: impact on signali…

2002

Abstract Among the anti-neutrophil cytoplasmic Abs (ANCA), those targeting proteinase 3 (PR3) have a high sensitivity and specificity for Wegener’s granulomatosis (WG). A pathogenetic role for these autoantibodies has been proposed due to their capacity of activating neutrophils in vitro. Recently, PR3 was also detected in human renal tubular epithelial cells (TEC). In the present study, the effect of murine monoclonal anti-PR3 Abs (anti-PR3) and purified c-ANCA targeting PR3 from WG serum on isolated human renal tubular cell signaling and inflammatory mediator release was characterized. Priming of TEC with TNF-α resulted in surface expression of PR3, as quantified in immunofluorescence stu…

EEG-Befunde bei ALL-Patienten 10 Jahre nach Bestrahlung und Zytostatikatherapie

1990

35 patients with acute lymphoblastic leukemia were studied electroencephalographically during a period of 5 to 10 years (median 9 years) after diagnosis. During this time 51% of the patients intermittently showed changes in the background activity, which may indicate discrete CNS-lesions. All but one patient, suffering from an intracerebral hemorrhage, had normal EEGs at the end of the individual observation period.

Preeclampsia is associated with defective production of C1q by invasive trophoblast

2011

The Friedreich's Ataxia protein frataxin modulates DNA base excision repair in prokaryotes and mammals

2010

DNA-repair mechanisms enable cells to maintain their genetic information by protecting it from mutations that may cause malignant growth. Recent evidence suggests that specific DNA-repair enzymes contain ISCs (iron–sulfur clusters). The nuclearencoded protein frataxin is essential for the mitochondrial biosynthesis of ISCs. Frataxin deficiency causes a neurodegenerative disorder named Friedreich's ataxia in humans. Various types of cancer occurring at young age are associated with this disease, and hence with frataxin deficiency. Mice carrying a hepatocyte-specific disruption of the frataxin gene develop multiple liver tumours for unresolved reasons. In the present study, we show that frata…

An Inhibitor of Cytochrome Oxidase Activity in the Sea Urchin Egg

1959

PREVIOUS work in this laboratory1,2 has demonstrated the presence of a highly active cytochrome oxidase in the isolated mitochondria of unfertilized sea urchin eggs. This activity is only 25 per cent lower than that of the mitochondria of the newly fertilized egg and up to the blastula stage2. No cytochrome oxidase activity has been found other than in the mitochondria1,3. On the other hand, it is known that the oxygen consumption of the unfertilized sea urchin egg is quite low; fertilization restores a normal respiratory level which increases continuously during the early development (for a discussion, see ref. 4). The suggestion was then made2 that in the unfertilized egg an extra-mitocho…

Prognostiskie faktori, kas ietekme kombinēta terapijās efektivitāti glioblastomas ārstēšana. Paula Stradiņa klīniskas universitātes slimnīcas Onkoloģ…

2015

Glioblastoma – visizplatītākais un visbīstamākais galvassmādzeņu primārs audzējs. To ārstē, izmantojot kombinēto ķīmij-stāru terapiju. Lai noprognozētu kombinēts terapijas efektivitāti un, secīgi, arī pacientā iespējamo dzīvildzi, jānosaka un jānovērtē iespējami prognostiskie faktori. Šajā pētījumā tika aplūkoti sekojošie prognostiskie faktori: pacientā vecums un dzimums, audzēja lokalizācija (labā vai kreisā puslodē), operācijas apjoms (rezēkcija vai biopsija) un KPS. Lai noskaidrotu šo faktoru ietekmi, tika pētīti 78 klīniskie gadījumi, informācija tika ņemta no pacientu slimības vēsturēm un medicīnas kartiņām. Dati tika apkopoti izmantojot MS Excel 2003 un MedCalc programmatūru. Gala rez…

Nanotechnology for Natural Medicine: Formulation of Neem Oil Loaded Phospholipid Vesicles Modified with Argan Oil as a Strategy to Protect the Skin f…

2021

Neem oil, a plant-derived product rich in bioactives, has been incorporated in liposomes and hyalurosomes modified by adding argan oil and so called argan-liposomes and argan-hyaluro-somes. Argan oil has also been added to the vesicles because of its regenerative and protective effects on skin. In the light of this, vesicles were specifically tailored to protect the skin from oxidative stress and treat lesions. Argan-liposomes were the smallest vesicles (~113 nm); the addition of sodium hyaluronate led to an increase in vesicle size (~143 nm) but it significantly improved vesicle stability during storage. In vitro studies confirmed the free radical scavenging activity of formula-tions, irre…

Melittin Modulates Keratinocyte Function through P2 Receptor-dependent ADAM Activation

2012

Melittin, the major component of the bee venom, is an amphipathic, cationic peptide with a wide spectrum of biological properties that is being considered as an anti-inflammatory and anti-cancer agent. It modulates multiple cellular functions but the underlying mechanisms are not clearly understood. Here, we report that melittin activates disintegrin-like metalloproteases (ADAMs) and that downstream events likely contribute to the biological effects evoked by the peptide. Melittin stimulated the proteolysis of ADAM10 and ADAM17 substrates in human neutrophil granulocytes, endothelial cells and murine fibroblasts. In human HaCaT keratinocytes, melittin induced shedding of the adhesion molecu…

Local administration of antisense phosphorothioate oligonucleotides to the c-kit ligand, stem cell factor, suppresses airway inflammation and IL-4 pr…

2001

Abstract Background: The c-kit ligand, stem cell factor (SCF), is an important activating and chemotactic factor for both mast cells and eosinophils. These cells are known to play a fundamental role in the pathogenesis of asthma. Objective: Our goal was to analyze the functional role of SCF in the pathogenesis of asthma. Methods: The expression of SCF was targeted in fibroblasts, epithelial cells, and locally in a murine model of asthma in mice induced by ovalbumin sensitization with an antisense DNA strategy. Results: We could suppress SCF expression in NIH 3T3 fibroblasts and SP1 epithelial cells by a specific antisense phosphorothioate oligonucleotide overlapping the translation start si…