Search results for "BOS"
showing 10 items of 3713 documents
Eukaryotic rRNA Modification by Yeast 5-Methylcytosine-Methyltransferases and Human Proliferation-Associated Antigen p120.
2015
International audience; Modified nucleotide 5-methylcytosine (m(5)C) is frequently present in various eukaryotic RNAs, including tRNAs, rRNAs and in other non-coding RNAs, as well as in mRNAs. RNA: m(5)C-methyltranferases (MTases) Nop2 from S. cerevisiae and human proliferation-associated nucleolar antigen p120 are both members of a protein family called Nop2/NSUN/NOL1. Protein p120 is well-known as a tumor marker which is over-expressed in various cancer tissues. Using a combination of RNA bisulfite sequencing and HPLC-MS/MS analysis, we demonstrated here that p120 displays an RNA:m(5)C-MTase activity, which restores m(5)C formation at position 2870 in domain V of 25S rRNA in a nop2 Delta …
Absolute quantification of noncoding RNA by microscale thermophoresis
2019
Abstract Accurate quantification of the copy numbers of noncoding RNA has recently emerged as an urgent problem, with impact on fields such as RNA modification research, tissue differentiation, and others. Herein, we present a hybridization‐based approach that uses microscale thermophoresis (MST) as a very fast and highly precise readout to quantify, for example, single tRNA species with a turnaround time of about one hour. We developed MST to quantify the effect of tRNA toxins and of heat stress and RNA modification on single tRNA species. A comparative analysis also revealed significant differences to RNA‐Seq‐based quantification approaches, strongly suggesting a bias due to tRNA modifica…
Valutazione della resistenza allo stress salino di Puccinellia distans (Jacq.) Parl. trattata con applicazioni fogliari dell'osmoregolatore glicinbet…
2009
Qualità e performance del tappeto erboso sportivo in diverse condizioni di ombreggiamento
Estudio de los mecanismos responsables del efecto protrombótico de Abacavir
2021
La terapia antirretroviral combinada es la que se utiliza para el tratamiento del virus de la inmunodeficiencia humana (VIH) y ha permitido que el síndrome de inmunodeficiencia adquirida (SIDA) haya pasado de ser una enfermedad mortal a crónica. Sin embargo, su uso se ha asociado con la aparición de diversos efectos adversos, entre ellos las enfermedades cardiovasculares. Debido a que los fármacos antirretrovirales se pautan de manera combinada, es difícil establecer la implicación de cada uno de los fármacos en estos efectos adversos. Abacavir (ABC) —inhibidor de la transcriptasa inversa análogo de nucleósido, concretamente de la guanina (purina endógena)— ha sido relacionado con un mayor …
Il trattamento domiciliare della TVP
2008
Formy kobiecej pamięci o doświadczeniu wojennym utrwalone w literaturze pięknej w Bośni i Hercegowinie
2020
The memory of the experience of the war in Bosnia and Herzegovina (1992–1995) is a phenomenon strongly present in contemporary literature. The subject of my interest is the specificity of women’s memory, which is characterized primarily by anti‑war and anti‑military discourse, which is a fundamental feature of women’s writing about the war, breaking the patriarchal code (statement by Swietlana Aleksijewicz). A notable feature of women’s creativity in BiH is that the memory of war is revealed in it in two fundamentally different ways: 1. a direct description of war experiences and experiences is usually visible in the texts of the authors of the older generation (Alma Lazarevska; Jasmina Mus…
Prevention of venous thromboembolism in patients with cancer: guidelines of the Italian Society for Haemostasis and Thrombosis
2012
An extended catalogue of ncRNAs in Streptomyces coelicolor reporting abundant tmRNA, RNase-P RNA and RNA fragments derived from pre-ribosomal RNA lea…
2022
Streptomyces coelicolor is a model organism for studying streptomycetes. This genus possesses relevant medical and economical roles, because it produces many biologically active metabolites of pharmaceutical interest, including the majority of commercialized antibiotics. In this bioinformatic study, the transcriptome of S. coelicolor has been analyzed to identify novel RNA species and quantify the expression of both annotated and novel transcripts in solid and liquid growth medium cultures at different times. The major characteristics disclosed in this study are: (i) the diffuse antisense transcription; (ii) the great abundance of transfer-messenger RNAs (tmRNA); (iii) the abundance of rnpB…
The LuxR Regulators PcoR and RfiA Co-regulate Antimicrobial Peptide and Alginate Production in
2017
Cyclic lipopeptides (CLPs) are considered as some of the most important secondary metabolites in different plant-associated bacteria, thanks to their antimicrobial, cytotoxic, and surfactant properties. In this study, our aim was to investigate the role of the Quorum Sensing (QS) system, PcoI/PcoR, and the LuxR-type transcriptional regulator RfiA in CLP production in the phytopatogenic bacterium, Pseudomonas corrugata based on our previous work where we reported that the pcoR and rfiA mutants were devoid of the CLPs cormycin and corpeptin production. Due to the close genetic link between the QS system and the RfiA (rfiA is co-transcribed with pcoI), it was difficult to ascertain the specifi…